Abstract
In this paper, we report the cloning and characterization of the first mannose-binding lectin gene from a gymnosperm plant species,Taxus media. The full-length cDNA ofT. media agglutinin (TMA) consisted of 676 bp and contained a 432 bp open reading frame (ORF) encoding a 144 amino acid protein. Comparative analysis showed that TMA had high homology with many previously reported plant mannose-binding lectins and thattma encoded a precursor lectin with a 26-aa signal peptide. Molecular modelling revealed that TMA was a new mannosebinding lectin with three typical mannose-binding boxes like lectins from species of angiosperms. Tissue expression pattern analyses revealed thattma is expressed in a tissue-specific manner in leaves and stems, but not in fruits and roots. Phylogenetic tree analyses showed that TMA belonged to the structurally and evolutionarily closely related monocot mannose-binding lectin superfamily. This study provides useful information to understand the molecular evolution of plant lectins.
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Abbreviations
- AAA:
-
Allium ascalonicum agglutinin
- ACA:
-
Allium cepa agglutinin
- ACPA:
-
Ananas comosus (pineapple) agglutinin
- AKA:
-
Amorphophallus konjac agglutinin
- APA:
-
Allium porrum agglutinin
- ASA:
-
Allium sativum agglutinin
- AUA:
-
Allium ursinum agglutinin
- AVA:
-
Amaryllis vittata agglutinin
- CAA:
-
Crinum asiaticum agglutinin
- CTAB:
-
cetyltrimethylammonium bromide
- EHA:
-
Epipactis helleborine agglutinin
- GEAFP:
-
Gastrodia elata antifungal protein
- GNA:
-
Galanthus nivalis agglutinin
- LOA:
-
Listera ovata agglutinin
- ORF:
-
open reading frame
- PCR:
-
polymerase chain reaction
- pI:
-
isoelectric point
- RACE:
-
rapid amplification of cDNA ends
- RT-PCR:
-
reverse transcriptase-polymerase chain reaction
- TDA:
-
Typhonium divaricatum agglutinin
- TMA:
-
Taxus media agglutinin
- UTR:
-
untrslated region
- ZCA:
-
Zephyrathes candida agglutinin
- ZGA:
-
Zephyrathes grandiflora agglutinin
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Kai, G., Zhao, L., Zheng, J. et al. Isolation and characterization of a new mannose-binding lectin gene fromTaxus media . J. Biosci. 29, 399–407 (2004). https://doi.org/10.1007/BF02712111
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DOI: https://doi.org/10.1007/BF02712111