Abstract
Pseudomonas sp. lipase was immobilized by adsorption onto five supports and tested for its ability to synthesize geranyl esters by transesterification using short-chain triacylglycerols as acyl donors. Reaction mixtures were prepared in 2 mL ofn-hexane, 0.1 M geraniol, 0.03M triacylglycerol, and 200 units of lipase, and incubated at 30°C and 200 rpm for 24 h. Overall, glass beads were the best support. Geranyl acetate and caproate performed best with Duolite (77.5 and 95.3%, respectively). Geranyl butyrate and caprylate performed best with polyvinylpyrrolidone, (80.2 and 95.5%, respectively). Values for nonimmobilized enzyme also were obtained. Immobilization improved yields, with geranyl caproate exhibiting best results.
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References
Claon, P.A., and C.C. Akoh,Biotechnol. Lett. 15:1211 (1993).
Claon, P.A., and C.C. Akoh,Enzyme Microb. Technol.16:835 (1994).
Gillies, B., H. Yamazaki and P.W. Armstrong,Biotechnol. Lett. 9:709 (1987).
Posorske, L.H.,J. Am. Oil Chem. Soc. 61:1758 (1984).
Rizzi, M., P. Stylos, A. Riek and M. Reuss,Enzyme Microb. Technol. 14:709 (1992).
Chulalaksananukul, W., J. Condoret and D. Combes, Ibid.:293 (1992).
Gray, C.J., J.S. Narang and S.A. Barker,Enzyme Microb. Technol. 12:800 (1990).
Claon, P.A., and C.C. Akoh,Biotechnol. Lett. 16:235 (1994).
Claon, P.A., and C.C. Akoh,J. Agric. Food Chem. 42:2349 (1994).
Yee, L.N., C.C. Akoh and R.S. Phillips,Biotechnol. Lett. 17:67 (1995).
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Yee, L.N., Akoh, C.C. & Phillips, R.S. Pseudomonas sp. lipase-catalyzed synthesis of geranyl esters by transesterification. J Am Oil Chem Soc 72, 1407–1408 (1995). https://doi.org/10.1007/BF02546220
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DOI: https://doi.org/10.1007/BF02546220