Summary
Candida cylindracea lipase adsorbed to silica gel produced a variety of flavor esters when hydrated and shaken in n-heptane containing substrates. Scale-up production of ethyl butyrate was examined in a packed column with recycling of n-hexane containing substrates. Increased substrate concentrations were stimulatory up to a point after which inhibition and enzyme destabilization in repeated runs occurred.
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Gillies, B., Yamazaki, H. & Armstrong, D.W. Production of flavor esters by immobilized lipase. Biotechnol Lett 9, 709–714 (1987). https://doi.org/10.1007/BF01024605
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DOI: https://doi.org/10.1007/BF01024605