Abstract
ATP receptor-mediated changes in the Ca2+ concentration were recorded from neurons of the sensorimotor cortex in brain slices from 3-week-old rats. To measure the cytoplasmic concentration of Ca2+, slices were incubated with Fura-2/AM, and a microfluorimetry system was focused on an individual cell. Possible glutamatergic signals resulting from ATP-evoked glutamate release were excluded. After elimination of calcium from the extracellular solution, the first ATP-induced [Ca2+] i transient decreased to 62±9% of a similar response in the normal solution, suggesting the participation of metabotropic purinoreceptor-triggered Ca release in transient generation. Depletion of the caffeine-sensitive calcium store results in diminution of ATP-induced [Ca2+] i transient in the Ca2+-free solution by 31.4±7.0% (P<0.01). This may indicate that in pyramidal neurons of the sensorimotor cortex InsP3- and Ca-induced Ca-releases demonstrate noticeable functional interaction. Nevertheless, there is no single compartment in the endoplasmic reticulum bearing both IICR and CICR channels.
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P. G. Kostyuk and A. N. Verkhratsky,Calcium Signalling in the Nervous System, John Wiley and Sons, Chichester (1995).
A. Shmigol, P. Kostyuk, and A. Verkhratsky, “Role of caffeinesensitive Ca2+ stores in Ca2+ signal termination in adult mouse DRG neurons,”NeuroReport,5, 2073–2076 (1994).
S. Kirschuk, N. Voitenko, P. Kostyuk, and A. Verkhratsky, “Calcium signalling in granule neurones studied in cerebellar slices,”Cell Calcium,19, No. 1, 59–71 (1996).
N. Svichar, A. Shmigol, A. Verkhratsky, and P. Kostyuk, “InsP3-induced Ca2+ release in dorsal root ganglion neurons,”Neuroscience,227, 107–110 (1997).
A. Shmigol, S. Kirischuk, P. Kostyuk, and A. Verkhratsky, “Different properties of caffeine-sensitive Ca2+ stores in peripheral and central mammalian neurons,”Pflügers Arch.,426, 174–176 (1994).
S. N. Murphy and R. J. Miller, “Two distinct quisqualate receptors regulate Ca2+ homeostasis in hippocampal neuronesin vitro,”Mol. Pharmacol.,35, 671–680 (1989).
T. Shirasaki, N. Harata, and N. Akaike, “Metabotropic glutamate response in acutely dissociated hippocampalCA1 pyramidal neurones of the rat,”J. Physiol.,475, 439–453 (1994).
A. J. Irving, G. L. Collingridge, and J. G. Schofiel, “Interactions between Ca2+ mobilizing mechanisms in cultured rat cerebellar granule cells,”J. Physiol.,456, 667–680 (1992).
G. Grynkiewicz, M. Poenie, and R. Y. Tsien, “A new generation of Ca2+ indicators with greatly improved fluorescent properties,”J. Biol. Chem.,260, 3440–3450 (1985).
G. Gu. Jianguo and B. Amy MacDermott, “Activation of ATP P2x receptors elicits glutamate release from sensory neuron synapses,”Nature,389, 749–753 (1997).
M. Hollman and S. Heinemann, “Cloned glutamate receptors,”Annu. Rev. Neurosci.,17, 31–108 (1994).
S. L. Mironov, “Metabotropic ATP receptor in hippocampal and thalamic neurons: pharmacology and modulation of Ca2+ mobilizing mechanisms,”Neuropharmacology,33, No. 1, 1–13 (1994).
S. Kirischuk, V. Matiash, A. Kulik, et al., “Activation of P2-purino-, α1-adreno and H1-histamine receptors triggers cytoplasmic calcium signalling in cerebellar Purkinje neurons,”Neuroscience,73, No. 3, 643–647 (1996).
N. Svichar, A. Shmigol, A. Verkhratsky, and P. Kostyuk, “ATP induces Ca2+ release from IP3-sensitive Ca2+ stores exclusively in large DRG neurones,”NeuroReport,8, 1555–1559 (1997).
Y. Hirano, F. Okajima, H. Tomura, et al., “Change in intracellular calcium of neural cell induced by extracellular ATP,”FEBS,284, No. 2, 235–237 (1991).
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Lalo, U.V., Kostyk, P.G. Depletion of caffeine-sensitive calcium store results in diminution of ATP-induced metabotropic calcium responses in rat neocortical neurons. Neurophysiology 30, 289–292 (1998). https://doi.org/10.1007/BF02462840
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DOI: https://doi.org/10.1007/BF02462840