Abstract
Particulate suspended matter in oceanic, coastal, and estuarine regions can be specifically marked immunochemically with a fluorescent probe using antisera recognizing antigens present on their surface. Of the particulate matter, phytoplankton is a major component. Toxic species that may form harmful blooms can be a direct threat to aquaculturing tourism, sea-life and man. In order to detect such species in natural fixed phytoplankton populations, immunochemical tagging has been combined with flow cytometric evaluation Microalgal cells can be labeled with a fluorescent probe (fluorescein isothiocyanate, FITC, is recommended). Labeled cells are counted using a flow cytometer. This method has proved to be applicable in a monitoring programme in the North Sea.
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ANDERSON, D.M., 1989. Toxic algal blooms and red tides: A global perspective. In: T. Okaichi, D.M. Anderson and T. Nemoto, Eds., Red Tides: Biology, environmental sciences, and toxicology. Elsevier, New York, p. 11–16.
ANDERSON, D.M., B.A. KEAFER, D.M. KULIS, R.M. WATERS and R. NUZZI, 1993. An immunofluorescent survey of the brown tide chrysophyteAureococcus anophagefferens along the northeast coast of the United States. J. Plankton Res., 15: 563–580.
BATES, S.S., C. LÉGER, B.A., KEAFER, and D.M. ANDERSON, 1993. Discrimination between domoic-acid producing and non-toxic forms of the diatomPseudonitzschia pungens using immunofluorescence. Mar. Ecol. Prog. Ser., 100: 185–195.
DUBELAAR, G.B.J., A.C. GROENEWEGEN, W. STOKDIJK, G.J. VAN DEN ENGH and J.W.M. VISSER, 1989. The optical plankton analyser (OPA): a flow cytometer for plankton analysis. II: Specifications. Cytometry, 10: 529–539.
HALL, J.A., 1991. Long-term preservation of picoplankton for counting by fluorescence microscopy. British Phycol. J., 26: 169–174.
HALLEGRAEFF, G.M., 1993. A review of harmful algal blooms and their apparent global increase. Phycologia, 32: 79–99.
HIROISHI, S., A. UCHIDA, K. NAGASAKI and Y. ISHIDA, 1988. A new method for identification of inter- and intra-species of the red tide algaeChattonella antiqua andChattonella marina (Raphidophyceae) by means of monoclonal antibodies. J. Phycol., 24: 442–444.
KOEMAN, R.P.T., T.W.M. RADEMAKER and A. BUMA, 1992. Biomonitoring of phytoplankton in Dutch salt and brackish waters. TRIPOS rapp., by order of Ministry of Public Works and Transport, Tidal Waters Division (In Dutch).
PEETERS, J.C.H., G.B.J. DUBELAAR, J. RINGELBERG and J.W.M. VISSER, 1989. The optical plankton analyser (OPA): a flow cytometer for plankton analysis. I: Design considerations. Cytometry 10: 522–528.
SMAYDA, T.J., 1990. Novel and nuisance phytoplankton blooms in the sea: Evidence for a global epidemic. In: E. Granéli, B. Sundström, L. Edler and D.M. Anderson, Eds. Toxic marine phytoplankton. Elsevier, New York, p. 29–39.
SMAYDA, T.J. and Y. SHIMIZU, 1993. Toxic phytoplankton blooms in the sea. Elsevier, Amsterdam.
VRIELING, E.G., A. DRAAIJER, W.J.M. van ZEIJL, L. PEPERZAK, W.W.C. GIESKES and M. VEENHUIS, 1993a. The effect of labeling intensity, estimated by real time confocal laser scanning microscopy, on flow cytometric appearance and identification of immunochemically labeled marine dinoflagellates. J. Phycol., 29: 180–188.
VRIELING, E.G., W.W.C. GIESKES, J.W., HOFSTRAAT, F. COLIJN, L. PEPERZAK and M. VEENHUIS 1993b. Immunochemical identification of toxic marine algae: First results usingProrocentrum micans as a model organism. In: T.J. Smayda and Y. Shimizu, Eds., Toxic phytoplankton blooms in the sea, Elsevier, Amsterdam, p. 925–931.
VRIELING, E.G., L. PEPERZAK, W.W.C. GIESKES and M. VEENHUIS, 1994. Detection of the ichthyotoxic dinoflagellateGyrodinium (cf.)aureolum and morphologically relatedGymnodinium species using monoclonal antibodies: a specific immunological tool. Mar. Ecol. Progr. Ser., 103: 165–174.
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Vrieling, E.G., Gieskes, W.W.C., Van Zeijl, W.J.M. et al. Detection of toxic phytoplankton species by immunochemical particle analysis based on flow cytometry. Netherlands Journal of Aquatic Ecology 28, 249–254 (1994). https://doi.org/10.1007/BF02334192
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DOI: https://doi.org/10.1007/BF02334192