Summary
Bisphosphoimidazolides of 2′-deoxycytidine and of its acyclic analog\(\tilde C\) can be oligomerized in aqueous solution in the presence of Mn(II). Under certain conditions, a range of products extending to at least the 20mer can be obtained. These products are of interest as possible templates for oligomerization of the complementary monomers.
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Abbreviations
- \(\tilde C\) :
-
1-[(1,3-dihydroxy-2-propoxy)methyl]cytosine\(p\tilde Cp\), the bisphosphate of\(\tilde C\)
- dN (N=C, T, G, or A):
-
the 2′-deoxynucleoside of cytosine, thymine, guanine, or adenine
- pdNp:
-
the 3′, 5′-bisphosphate of dN; ImpdNpIm, the 3′, 5′-bisphosphoimidazolide of dN
- oligo(pdCp):
-
3′, 5′-linked oligomers of pdCp
- 2-MeImpG:
-
the 5′-phospho-2-methylimidazolide of G
- EDTA:
-
ethylenediamine tetraacetic acid
- Tris:
-
tris(hydroxymethyl)aminomethane
- Bis-Tris:
-
bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane
References
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Visscher, J., Schwartz, A.W. Oligomerization of cytosine-containing nucleotide analogs in aqueous solution. J Mol Evol 30, 3–6 (1990). https://doi.org/10.1007/BF02102447
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DOI: https://doi.org/10.1007/BF02102447