Summary
Osteoclastic bone resorption involves the solubilization of the mineral salts and the degradation of noncollagen bone matrix and collagen fibrils. As no recognizable collagen fibrils have ever been reported within cytoplasmic vacuoles in osteoclasts, it is generally assumed that the collagen fibrils are digested extracellularly in the resorption zone. The extent to which lysis occurs extracellularly and whether or not the osteoclasts phagocytose the degradation products remain to be established.
In the present communication, a hypothesis is presented suggesting the possibility that osteoclastic resorption of bone involves the participation of two different cell types. According to this hypothesis, osteoclastic bone resorption is initiated by osteoclasts that demineralize areas of bone and degrade noncollagen bone matrix. After the osteoclasts have moved away or become partially detached from the demineralized site, the exposed collagen fibrils are phagocytosed by mononuclear, fibroblast-like or monocyte-derived cells.
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Hancox, N.M.: The osteoclast. In: The biochemistry and physiology of bone (Bourne, G.M., ed.), p. 45–69. New York: Academic Press 1972
Bonucci, E.: The organic-inorganic relationships in bone matrix undergoing osteoclastic resorption. Calcif. Tissue Res.16, 13–36 (1974)
Holtrop, M.E., King, G.J.: The ultrastructure of the osteoclast and its functional implications. Clin. Orthop.123, 177–196 (1977)
Goldhaber, P.: Oxygen-dependent bone resorption in tissue culture. In: The parathyroids (Greep, R.O., Talmage, R.V., eds.), pp. 243–254. Springfield, Ill.: Charles C. Thomas 1961
Mundy, G.R., Altman, A.J., Gondek, M.D., Bandelin, J.G.: Direct resorption of bone by human monocytes. Science196, 1109–1111 (1977)
Bélanger, L.T.: Osteocytic resorption. In: The biochemistry and physiology of bone (Bourne, G.H., ed.), Vol. II, Ed. 2, pp. 239–270. New York: Academic Press 1971
Heersche, J.N.M.: The actions of parathyroid hormone and thyrocalcitonin on bone demineralization and on resorption of bone collagen. Calcif. Tissue Res. [Suppl.]2, 80 (1968)
Heersche, J.N.M.: The effect of thyrocalcitonin and parathyroid hormone on bone metabolism in tissue culture: the effect on collagen breakdown in relation to bone demineralization. Proc. Kon. Ned. Akad. Wet.C72, 286–298 (1969)
Heersche, J.N.M.: The effect of thyrocalcitonin and parathyroid hormone of bone metabolism in tissue culture: the effects of parathyroid hormone and thyrocalcitonin on the process of bone demineralization. Proc. Kon. Ned. Akad. Wet.C72, 594–606 (1969)
Brand, J.S., Raisz, L.G.: Effects of thyrocalcitonin and phosphate ion on the parathyroid hormone stimulated resorption of bone. Endocrinology90, 479–487 (1972)
Wong, C.L., Cohn, D.V.: Target cells in bone for parathormone and calcitonin are different: enrichment for each cell type by sequential digestion of mouse calvaria and selective adhesion to polymeric surfaces. Proc. Natl. Acad. Sci. U.S.A.72, 3167 (1975)
Luben, R.A., Wong, G.L., Cohn, D.A.: Biochemical characterization with parathormone and calcitonin of isolated bone cells: provisional identification of osteoclasts and osteoblasts. Endocrinology99, 526–534 (1976)
Holtrop, M.E., Raisz, L.G., Simmons, H.A.: The effect of parathyroid hormone, colchicine and calcitonin on the ultrastructure and activity of osteoclasts in organ culture. J. Cell Biol.60, 346–355 (1974)
Kallio, D.M., Garant, P.R., Minkin, C.: Evidence of coated membranes in the ruffled boarder of osteoclasts. J. Ultrastruct. Res.37, 169–177 (1971)
Irving, J.T., Handelman, C.S.: Bone destruction by multi-nucleated giant cells. In: Mechanisms of hard tissue destruction (Sognaes, R.F., ed.), pp. 515–530. Washington, D.C.: Amer. Assoc. Adv. Sci. Pub. (No. 75) 1963
Irving, J.T., Heeley, J.D.: Resorption of bone collagen by multinucleated cells. Calcif. Tissue Res.6, 254–259 (1970)
Rasmussen, H., Bordier, P.: The physiological and cellular basis of metabolic bone disease. Baltimore: Williams & Wilkins Co. 1974
Gaillard, P.J.: Parathyroid gland tissue and bone in vitro I. Exp. Cell Res. [Suppl.]3, 154–169 (1955)
Gaillard, P.J.: Parathyroid gland tissue and bone in vitro II. Proc. Kon. Ned. Akad. Wet.C58, 279–293 (1955)
Gaillard, P.J.: Parathyroid and bone in tissue culture. In: The parathyroids (Greep, R.O., Talmage, R.V., eds.), pp. 20–48. Springfield, Ill.: Charles C. Thomas 1961
Lucht, M.: Absorption of peroxidase by osteoclasts as studied by electronmicroscope histochemistry. Histochemistry29, 274–286 (1972)
Luse, S., Hutton, R.: An electron microscopic study of the fate of collagen in the post-partum rat uterus. Anat. Rec.148, 308 (1964)
Brandes, D., Anton, E.: An electron microscopic cytochemical study of macrophages during uterine involution. J. Cell Biol.41, 450–461 (1969)
Parakkal, P.: Involvement of macrophages in collagen resorption. J. Cell Biol.41, 345–354 (1969)
Ten Cate, A.R.: Morphological studies of fibrocytes in connective tissue undergoing rapid remodelling. J. Anat. (Lond.)112, 401–414 (1972)
Listgarten, M.A.: Intracellular collagen fibrils in the periodontal ligament of the mouse, rat, hamster, guinea pig and rabbit. J. Periodont. Res.8, 335–342 (1973)
Beertsen, W., Everts, V., van den Hoof, A.: Fine structure of fibroblasts in the periodontal ligament of the rat incisor and their possible role in tooth eruption. Arch. Oral Biol.19, 1087–1098 (1974)
Ten Cate, A.R., Deporter, D.A.: The role of the fibroblast in collagen turnover in the functioning periodontal ligament of the mouse. Arch. Oral Biol.19, 339–340 (1974)
Gothlin, G., Ericsson, J.L.E.: Electron microscopic studies of cytoplasmic filaments and fibers in different cell types of fracture callus. Acta Pathol. Microbiol. Scand.81, 532–542 (1970)
Cullen, J.C.: Intracellular collagen in experimental arthritis in rats. J. Bone Joint Surg.54B, 351–359 (1972)
Ten Cate, A.R., Freeman, E.: Collagen remodelling by fibroblasts in wound repair. Preliminary observations. Anat. Rec.179, 543–546 (1974)
Mundy, G.R., Eilon, G., Altman, A.J.: Non-bone cell mediated bone resorption. In: Mechanisms of localized bone loss (Horton, J.E., Tarpley, T.M. Jr., Davis, W.F. Jr., eds.). Calcif. Tissue Res. [Suppl.], June 1978. (in press)
Teitelbaum, S.L., Stewart, C.C., Kahn, A.J.: Contact-mediated bone resorption by human monocytes in vitro. In: Mechanisms of localized bone loss (Horton, J.E., Tarpley, T.M. Jr., Davis, W.F. Jr., eds.). Calcif. Tissue Res. [Suppl.], June 1978. (in press)
Garant, P.R.: Collagen resorption by fibroblasts. A theory of fibroblastic maintenance of the periodontal ligament. J. Periodontol.47, 380–390 (1976)
Sakamoto, S., Sakamoto, M., Goldhaber, P., Glimcher, M.J.: Localization of tissue collagenase in bone by indirect immunofluorescent antibody technique. In: Endocrinology of Calcium Metabolism (Copp, D.H., Talmage, R.V., eds.), p. 378. Amsterdam-Oxford: Excerpta Medica 1978.
Sakamoto, S., Sakamoto, M., Goldhaber, P., Glimcher, M.: Collagenase and bone resorption: isolation of collagenase from culture medium containing serum after stimulation of bone resorption by addition of parathyroid hormone extract. Biochim. Biophys. Res. Commun.63, 172–178 (1975)
Lenaers-Clays, G., Vaes, G.: Collagenase, procollagenase and bone resorption: effects of parathyroid hormone and calcitonin. Arch. Int. Physiol. Biochem.84, 636–637 (1976)
Vaes, G.: Lysosomes and the cellular physiology of bone resorption. In: Lysosomes in biology and pathology (Dingle, J.T., Fell, H.B., eds.), pp. 217–253. Amsterdam: North Holland Publishing Co. 1969
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Heersche, J.N.M. Mechanism of osteoclastic bone resorption: A new hypothesis. Calc. Tis Res. 26, 81–84 (1978). https://doi.org/10.1007/BF02013238
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DOI: https://doi.org/10.1007/BF02013238