Abstract
The mutation of L5178Yc and L5178Yd cells in culture provides the capability of testing known compounds for mutagenic activity. Since the cells also reproduce in the ascitic cavity of genetically compatible mice and are single cell infective, a murine host-mediated assay may also be done. The procedures required for the mutagenicity test in culture and in mice are outlined in (A) and (B). The softagar cloning technique is described in (C) and recent developments in culture techniques are presented in (D) of this article.
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References
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Glenn A. Fischer, Se Yong Lee andPaul Calabresi, unpublished results.
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G. A. Fischer andM. Y. Chu, unpublished results.
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Fischer, G.A. The host-mediated mammalian cell assay. Agents and Actions 3, 93–98 (1973). https://doi.org/10.1007/BF01986540
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DOI: https://doi.org/10.1007/BF01986540