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Methods to Study Tumor Surveillance Using Tumor Cell Transplantation into Genetically Engineered Mice

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Mouse Models of Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1267))

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Abstract

When a tumor evolves, there is constant crosstalk between the transformed cells and cells of the immune system. Transplantation of well-established tumor cell lines into genetically engineered mice is a valuable tool to study the contribution of a gene of interest to tumor surveillance. These methods bear several advantages: first, such cell lines are well characterized; second, much data for reference exist; and third, the impact of the immune system can be separated from tumor cell intrinsic effects. Here, we provide protocols for tumor cell transplantations to address the role of a specific gene product in tumor surveillance. We furthermore describe several approaches to define the impact of natural killer cells and T cells, such as cell depletion and adoptive transfer experiments or use of different genetically modified mice.

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Acknowledgement

Financial support for this project was provided by the St. Anna Kinderkrebsforschung, Children’s Cancer Research Institute, financing the position of EB. BS is supported by the Austrian Science Fund (FWF) projects SFB-F28 and P25642-B22.

Author information

Authors and Affiliations

  1. Ludwig Boltzmann Institute for Cancer Research, Vienna, Austria

    Eva Bauer

  2. Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Vienna, Austria

    Agnieszka Witalisz & Birgit Strobl

  3. Institute of Pharmacology, Medical University of Vienna, Währingerstrasse 13A, Vienna, 1090, Austria

    Dagmar Stoiber

  4. Ludwig Boltzmann Institute for Cancer Research, Ludwig Boltzmann Gesellschaft, Währingerstrasse 13A, Vienna, 1090, Austria

    Dagmar Stoiber

Authors
  1. Eva Bauer
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  2. Agnieszka Witalisz
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  3. Birgit Strobl
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  4. Dagmar Stoiber
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Corresponding author

Correspondence to Dagmar Stoiber .

Editor information

Editors and Affiliations

  1. Medical University Vienna (MUV) Institute for Cancer Research, Vienna, Austria

    Robert Eferl

  2. Research (LBI-CR), Medical University Vienna (MUV), Center of Physiology and Pharmacology & Ludwig Boltzmann Institute for Cancer, Vienna, Austria

    Emilio Casanova

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Bauer, E., Witalisz, A., Strobl, B., Stoiber, D. (2015). Methods to Study Tumor Surveillance Using Tumor Cell Transplantation into Genetically Engineered Mice. In: Eferl, R., Casanova, E. (eds) Mouse Models of Cancer. Methods in Molecular Biology, vol 1267. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2297-0_22

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  • DOI: https://doi.org/10.1007/978-1-4939-2297-0_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2296-3

  • Online ISBN: 978-1-4939-2297-0

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