Abstract
Both in vivo and in vitro methodologies have uses as cytogenetic test systems for mutagenicity testing. The in vivo methods are certainly the choice as the primary test system, except perhaps in the case of viruses and some biologicals. When an in vitro method is used anaphase offers some advantages as greater speed and ease of observation, the detection of defects produced in G2 and mitosis, and the ability to distinguish between gaps and breaks, while it sacrifices the morphologic detail and ability to localize defects to specific chromosomes found in metaphase.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ad hoc Committee Report,Chromosome Methodologies in Mutation Testing, Toxic. appl. Pharmac.22, 269 (1972).
H. J. Evans,Chromosome Aberrations Induced by Ionizing Radiations, in:International Review of Cytology, vol. 13 (Ed. Bourne and Danielli: Academic Press, N.Y. 1962).
B. A. Kihlman,Sub-Chromatid Exchanges and the Strandedness of Chromosomes, Hereditas65, 171 (1970).
B. A. Kihlman,Molecular Mechanisms of Chromosome Breakage and Rejoining, Adv. Cell Molec. Biol. 1, 59 (1971).
D. E. Lea,Actions of Radiations on Living Cells (Cambridge University Press, N.Y. 1962).
W. W. Nichols, in:Drug Induced Diseases, vol. 4 (Eds. L. Meyler and H. M. Peck; Excerpta Medica, Amsterdam 1972), p. 59.
K. A. Palmer, G. F. Kelly andM. S. Legator,Method for Preparing Anaphase Figures from Rat Bone Marrow, Mt. Res.16, 111 (1972).
K. Sax,An Analysis of X-ray Induced Chromosomal Aberrations in Tradescantia, Genetics25, 41 (1940).
A. H. Sparrow,Types of Ionizing Radiation and Their Cytogenetic Effects, in:Mutation and Plant Breeding (Publ. 891, Natl. Acad. Sci., Natl. Res. Council, Washington, D.C.), p. 55.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nichols, W.W. Cytogenetic techniques in mutagenicity testing. Agents and Actions 3, 86–92 (1973). https://doi.org/10.1007/BF01986539
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01986539