Abstract
A liquid culture system for culturing detached spikes of barley (Hordeum vulgare L.) at different nutritional levels was established. The synthesis of hordein polypeptides was studied by pulse-labeling with [14C]sucrose at different stages of development and nitrogen (N) nutrition. All polypeptides were synthesised at 10 d after anthesis and hercafter an increase was observed for all polypeptides. A fivefold increase in total hordein was observed within the N range tested. Hordein-1 increased considerably more than hordein-2 with increased N nutrition, and hordein-1 synthesis exceeded that of hordein-2 at the highest N level 20 and 25 d after anthesis. Hordein-1 thus appears to act as the main N sink at high N levels. The synthesis of the major groups of hordein-2 polypeptides responded differently to increasing N in that the slower-migrating polypeptides increased more with increasing N than the faster-migrating polypeptides.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
References
Bishop, L.R. (1928) First report on barley proteins. The composition and qualitative estimation of barley proteins. J. Inst. Brew. (London)34, 101–118
Bishop, L.R. (1929) Second report on barley proteins. Composition and quantitative estimation of the barley proteins II. J. Inst. Brew. (London)35, 316–322
Bishop, L.R. (1930) Fourth report on barley proteins. The proteins of barley during development and storage and in the mature grain. The composition and quantitative estimation of the barley proteins III. J. Inst. Brew. (London)36, 336–349
Brandt, A. (1976) Endosperm formation during kernel development of wild type and high lysine barley mutant. Cereal Chem.53, 890–901
Chamberlain, J.P. (1979) Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal. Biochem.98, 132–135
Doll, H. (1980) A nearly non-functional mutant allele of the storage protein locus Hor 2 in barley. Hereditas93, 217–222
Doll, H., Andersen, B. (1981) Preparation of barley storage protein, hordein, for analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal. Biochem.115, 61–66
Doll, H., Brown, A.H.D. (1979) Hordein variation in wild (Hordeum spontaneum) and cultivated (H. vulgare) barley. Can. J. Genet. Cytol.21, 391–404
Donovan, G.R., Lee, J.W. (1977) Growth of detached wheat heads in liquid culture. Plant Sci. Lett.9, 107–113
Donovan, G.R., Lee, J.W. (1978) Effect of the nitrogen source on grain development in detached wheat heads in liquid culture. Aust. J. Plant Physiol.5, 81–87
Faulks, A.J., Shewry, P.R., Miflin, B.J. (1981) The polymorphism and structural homology of storage polypeptides (hordein) coded by the Hor-2 locus in barley (Hordeum vulgare L.). Biochem. Genet.19, 841–858
Jensen, C.J. (1977) Monoploid production by chromosome elimination. In: Applied and fundamental aspects of plant cell, tissue, and organ culture, pp. 299–330, Reinert, J., Bajaj, Y.P.S., eds. Springer, Berlin Heidelberg New York
Jensen, J., Jørgensen, H.J., Jensen, H.P., Giese, H., Doll, H. (1980) Linkage of the hordein loci Hor 1 and Hor 2 with the powdery mildew resistance loci Ml-k and Ml-a on barley chromosome 5. Theor. Appl. Genet.58, 27–31
Kirkman, M.A., Shewry, P.R., Miflin, B.J. (1982) The effect of nitrogen nutrition on the lysine content and protein composition of barley seeds. J. Sci. Food Agric.33, 115–127
Køie, B., Ingversen, J., Andersen, A.J., Doll, H., Eggum, B.O. (1976) Composition and nutritional quality of barley protein. In: Evaluation of seed protein alterations by mutation breeding, pp. 55–61. IAEA, Vienna
Laskey, R.A., Bonner, W.M. (1975) Quantitative film detection of H and14C in polyacrylamide gels by fluorography. Eur. J. Biochem.56, 335–341
Osborne, T.B. (1885) The proteins of barley. J. Am. Chem. Soc.17, 539–567
Rahman, S., Shewry, P.R., Miflin, B.J. (1982) Differential protein accumulation during barley grain development. J. Exp. Bot.33, 717–728
Shewry, P.R., Faulks, A.J., Pickering, R.A., Jones, I.T., Finch, R.A., Miflin, B.J. (1980a) The genetic analysis of barley storage proteins. Heredity44, 383–389
Shewry, P.R., Field, J.M., Kirkman, M.A., Faulks, A.J., Miflin, B.J. (1980b) The extraction, solubility and characterization of two groups of barley storage polypeptides. J. Exp. Bot.31, 393–407
Shewry, P.R., Pratt, H.M., Leggatt, M., Miflin, B.J. (1979) Protein metabolism in developing endosperms of high lysine and normal barley. Cereal Chem.56, 110–117
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Giese, H., Andersen, B. & Doll, H. Synthesis of the major storage protein, hordein, in barley. Planta 159, 60–65 (1983). https://doi.org/10.1007/BF00998815
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00998815