Summary
The results of this investigation indicate that neurofilaments of mammals (human and rabbit) are composed of four protofilaments each of which is formed of globular units connected by longitudinal bars. A cross-view of neurofilaments reveals the presence of four globular units, 20–25 Å in diameter, connected by four transverse bars, 25–30 Å in length. Two of the transverse bars appear more electrondense (darker) than the remaining two. A longitudinal view of neurofilaments shows two protofilaments bonded by the darker of the transverse bars to form a two-stranded band. The remaining two protofilaments form a band in a similar manner. The two two-stranded bands, wrapped helically, form a four-stranded neurofilament. The axial periodicity between twists along each neurofilament ranges from 200 Å to 270 Å. A three-dimensional diagram of neurofilament structure is presented.
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Delacourte A, Dousti M, Loucheux-Lefebvre MH (1982) Characterization of mammalian neurofilament subunits by circular dichroism. Biochim Biophys Acta 709:99–104
Gilbert DS (1975) Axoplasm architecture and physical properties as seen in theMyxicola giant axon. J Physiol (Lond) 253:257–301
Henderson DN, Geisler N, Weber K (1982) A periodic ultrastructure in intermediate filaments. J Mol Biol 155:173–176
Hoffman PN, Lasek RJ (1975) The slow component of axonal transport-identification of major structural polypeptides of the axon and their generality among mammalian neurons. J Cell Biol 66:351–366
Krishnan N, Kaiserman-Abramof IK, Lasek RJ (1979) Helical substructure of neurofilaments isolated fromMyxicola and squid giant axons. J Cell Biol 82:323–335
Lasek RJ, Krishnan N, Kaiserman-Abramof IR (1979) Identification of the subunit proteins of 10 nm neurofilaments isolated from axoplasm of squid andMycxicola giant axons. J Cell Biol 82:336–346
Metuzals J (1969) Configuration of a filamentous network in the axoplasm of squid. J Cell Biol 43:480–498
Metuzals J, Izzard CS (1969) Spatial patterns of thread-like elements in the axoplasm of the giant nerve fibers of the squid as disclosed by differential interference microscope. J Cell Biol 43:456–479
Metzals J, Muskynski WE (1974) Electron microscope and experimental investigation of the neurofilamentous network in deiters neurons. J Cell Biol 61:701–22
Metuzals J, Clapin DF (1981) Modes of crosslinking in neurofilament protein isolated from squid giant axon: electronmicroscopic evidence for paracrystalline arrays. Biol Bull 161:308 [Abstr]
Metuzals J, Clapin DF, Chapman GD (1982) Axial and radial filamentous components of the neurofilamentous network. Cell Tissue Res 223:507–518
Milam L, Erickson HP (1982) Visualization of a 21 nm axial periodicity in shadowed keratin filaments and neurofilaments. J Cell Biol 94:592–596
Osborn M, Weber K (1982) Intermediate filaments: cell-type-specific markers in differentiation and pathology. Cell 31:303–306
Phillips LL, Lasek RJ, Autilio-Gambetti L (1983) Evolutionary patterns of molecular conservation and variation in neurofilament protein. Sco Neurosci 9:343 [Abstr]
Roslansky PF, Cornell-Bell A, Rice RV, Adelman WJ Jr (1980) Polypeptide composition of squid neurofilaments. Proc Natl Acad Sci USA 77:404–408
Schlaepfer WW (1977) Studies on the isolation and substructure of mammalian neurofilaments. J Ultrastruct Res 61:149–157
Schmitt O, Davidson PE (1961) Biologie moleculaire des neurofilaments. In: Mounier AM (ed) Actualités neurophysiologiques. Masson Paris, pp 355–369
Soifer D, Iqbal K, Czosnek H, DeMartini J, Sturman JA, Wisniewski HM (1981) The loss of neuron-specific protein during the course of Wallerian degeneration of optic and sciatic nerve. J Neurosci 1:461–470
Spurr AR (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Wais-Steider C, Ealges PAM, Gilbert DS, Hopkins JM (1983) Structural similarities and differences amongst neurofilaments. J Mol Biol 165:393–400
Willard M, Simon C (1981) Antibody decoration of neurofilaments. J Cell Biol 89:198–205
Wisniewski K, Czosnek H, Wisniewski HM, Soifer D, Ramos PL, Kim KS, Iqbal K (1982) Reduction of neuronal specific protein and some neurotransmitters in the infantile neuroaxonal dystrophy (INAD). Neuropediatrics 13:123–129
Wuerker RB (1970) Neurofilaments and glial filaments. Tissue Cell 2:1–9
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Supported in part by grans from the NIA (1 POI AGO 4220) and from the Aluminum Association (914-1065A)
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Wen, G.Y., Wisniewski, H.M. Substructures of neurofilaments. Acta Neuropathol 64, 339–343 (1984). https://doi.org/10.1007/BF00690399
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DOI: https://doi.org/10.1007/BF00690399