Summary
Almost all phospholipids are hydrolysed by treatment with N KOH at 37° for 16 hrs followed by 10% trichloracetic acid at 37° over a 3-day-period. After this treatment there remains only sphingomyelin and probably certain phospholipids of a type which occurs only in exceptionally small quantities in the CNS. This method is proposed for the estimation of the „sphingomyelin” content. This term is to be set between quotation marks to draw special attention to the small quantity of phospholipids apart from sphingomyelin.
Résumé
Si un mélange de phospholipides est traité durant seize heures à 37° avec KOH N et ensuite pendant trois jours à 37° avec 10% d'acide trichloracetique, presque tous les phospholipides seront hydrolysés. Ne résisteront à ce traitement que la sphingomyéline et peut-être quelques phospholipides qui ne sont présentes que dans des quantités minimes dans le système nerveux normal. Les auteurs proposent ce procédure pour estimer le taux de «sphingomyéline».
Ce terme a été mis entre guillemets pour attirer l'attention sur la présence de petites quantités de phospholipides autres que shpingomyéline.
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This investigation was supported by grants from the Dutch Health Research Council, T.N.O., and from the American National Multiple Sclerosis Society.
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Edgar, G.W.F., Mazure, M. & Smits, G. Revised procedure for the determination of „sphingomyelin” in fresh and formaldehyde-preserved tissue. Acta Neuropathol 3, 490–493 (1964). https://doi.org/10.1007/BF00688457
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DOI: https://doi.org/10.1007/BF00688457