Abstract
Lipids are a heterogeneous group of substances characterized by their solubility in organic solvents and insolubility in water. Lipids can be found as normal components of different tissues and organs, and they can be affected by several pathological conditions. The histochemical identification of lipids plays an important role in histopathological diagnosis and research, but successful staining depends on adequate fixation and processing of the tissue. Here we describe methods to fix and process tissue samples for the histochemical identification of lipids in frozen or paraffin-embedded tissues.
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References
Kiernan JA (2015) Histological and histochemical methods: theory and practice, 5th edn. Scion, Banbury
Mills SE (2007) Histology for pathologists, 3rd edn. Lippincott Williams & Wilkins, Philadelphia, PA
Carriel V, Garzón I, Alaminos M et al (2011) Evaluation of myelin sheath and collagen reorganization pattern in a model of peripheral nerve regeneration using an integrated histochemical approach. Histochem Cell Biol 136(6):709–717
Kiernan JA (2007) Histochemistry of staining methods for normal and degenerating myelin in the central and peripheral nervous systems. J Histotechnol 30(2):87–106
Kumar V, Abbas AK, Aster J (2013) Robbins basic pathology, 9th edn. Elsevier Saunders, Philadelphia, PA
Carriel V, Garzón I, Alaminos M et al (2014) Histological assessment in peripheral nerve tissue engineering. Neural Regen Res 9(18):1657–1660
Cook DJ, Warren PJ (2015) Cellular pathology: introduction to techniques and applications, 3rd edn. Scion, Bloxham
Crocker J, Burnett D (2005) The science of laboratory diagnosis, 2nd edn. John Wiley, Chichester
Carriel V, Campor A, Alaminos M, Raimondo S, Geuna S (2017) Staining methods for normal and regenerative myelin in the nervous system. Methods Mol Biol Volumen 1560, Histochemistry of single molecule, methods and protocols. ISBN: 978-1-4939-6787-2
Di Scipio F, Raimondo S, Tos P et al (2008) A simple protocol for paraffin-embedded myelin sheath staining with osmium tetroxide for light microscope observation. Microsc Res Tech 71(7):497–502
Tracy RE, Walia P (2002) A method to fix lipids for staining fat embolism in paraffin sections. Histopathology 41(1):75–9
Tracy RE, Walia P (2004) Lipid fixation for fat staining in paraffin sections applied to lesions of atherosclerosis. Virchows Arch 445(1):22–26
Meier-Ruge WA, Bruder E (2005) Pathology of chronic constipation in pediatric and adult coloproctology. Pathobiology 72(1-2):1–102
Serrato D, Nieto-Aguilar R, Garzón I et al (2009) Comparison of the effect of cryopreservation protocols on the histology of bioengineered tissues. Histol Histopathol 24(12):1531–1540
Acknowledgments
The methods described in this chapter were supported by the Spanish “Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica”, from the National Ministry of Economy and Competitiveness (Instituto de Salud Carlos III), Grant No FIS PI14-1343 and cofinanced by “Fondo Europeo de Desarrollo Regional” (FEDER), European Union; The Tissue Engineering Group (CTS-115) of the University of Granada, Spain; and by the “Fundación Benéfica Anticáncer San Francisco Javier y Santa Cándida, Granada, España”.
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Carriel, V., Campos, F., Aneiros-Fernández, J., Kiernan, J.A. (2017). Tissue Fixation and Processing for the Histological Identification of Lipids. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_14
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DOI: https://doi.org/10.1007/978-1-4939-6788-9_14
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Publisher Name: Humana Press, New York, NY
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