Abstract
Southern blot hybridization techniques were used to examine the chloroplast DNA (cpDNA) sequences present in the mitochondrial DNAs (mtDNAs) of two Brassica species (B. campestris and B. hirta), two closely related species belonging to the same tribe as Brassica (Raphanus sativa, Crambe abyssinica), and two more distantly related species of crucifers (Arabidopsis thaliana, Capsella bursa-pastoris). The two Brassica species and R. sativa contain roughly equal amounts (12–14 kb) of cpDNA sequences integrated within their 208–242 kb mtDNAs. Furthermore, the 11 identified regions of transferred DNA, which include the 5′ end of the chloroplast psaA gene and the central segment of rpoB, have the same mtDNA locations in these three species. Crambe abyssinica mtDNA has the same complement of cpDNA sequences, plus an additional major region of cpDNA sequence similarity which includes the 16S rRNA gene. Therefore, except for the more recently arrived 16S rRNA gene, all of these cpDNA sequences appear to have entered the mitochondrial genome in the common ancestor of these three genera. The mitochondrial genomes of A. thaliana and Capsella bursa-pastoris contain significantly less cpDNA (5–7 kb) than the four other mtDNAs. However, certain cpDNA sequences, including the central portion of the rbcL gene and the 3′ end of the psaA gene, are shared by all six crucifer mtDNAs and appear to have been transferred in a common ancestor of the crucifer family over 30 million years ago. 1n conclusion, DNA has been transferred sequentially from the chloroplast to the mitochondrion during crucifer evolution and these cpDNA sequences can persist in the mitochondrial genome over long periods of evolutionary time.
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References
Ayliffe MA, Timmis JN, Scott NS (1988) Theor Appl Genet 75:282–285
Cronquist A (1981) An integrated system of classification of flowering plants. Columbia University Press, New York, p 449
Dormann-Przybyl D, Strittmatter G, Kössel H (1986) Plant Mot Biol 7:419–431
Ellis J (1982) Nature 299:678–679
Giese K, Subramanian AR, Larrinua IM, Bogorad L (1987) J Biol Chem 262:15251–15255
Heywood VH (1985) Flowering plants of the world. Prentice-Hall, Englewood Cliffs, NJ, p 119
Kemble RJ, Mans RJ, Gabay-Laughan S, Laughnan JR (1983) Nature 304:744–747
Kirsch W, Seyer P, Herrmann RG (1986) Curr Genet 10:843–855
Kolodner R, Tewari KK (1972) Proc Natl Acad Sci USA 69:1830–1834
Lebacq P, Vedel F (1981) Plant Sci Lett 23:1–9
Lonsdale DM (1988) In: The Biochemistry of plants, vol 11. Springer, Berlin Heidelberg New York (in press)
Makaroff CA, Palmer JD (1987) Nucleic Acids Res 15:5141–5156
Marechal L, Runeberg-Roos P, Grienenberger J-M, Colin J, Weil J-H, Lejeune B, Quetier F, Lonsdale DM (1987) Curr Genet 12:91–98
Matsubayashi T, Wakasugi T, Shinozaki K, Yamaguchi-Shinozaki K, Zaita N, Hidaka T, Meng BY, Ohto C, Tanaka M, Kato A, Maruyama T, Sugiura M (1987) Mol Gen Genet 210:385–393
Meng BY, Matsubayashi T, Wakasugi T, Shinozaki K, Sugiura M, Hirai A, Mikami T, Kishuna Y, Kinoshita T (1986) Plant Sci 47:181–184
Ohme M, Tanaka M, Chunwongse J, Shinozaki K, Sugiura M (1986) FEBS Lett 200:87–90
Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:572–574
Palmer JD (1985) Molecular evolutionary genetics. In: MacIntyre RJ (ed) Monographs in evolutionary biology. Plenum Press, New York, p 131–240
Palmer JD (1986) Methods Enzymol 118:167–186
Palmer JD (1988) Genetics 118:341–351
Palmer JD, Herbon LA (1986) Nucleic Acids Res 14:9755–9765
Palmer JD, Herbon LA (1987) Curr Genet 11:565–570
Palmer JD, Herbon LA (1988) J Mol Evol 28 (in press)
Palmer JD, Shields CR (1984) Nature 307:437–440
Palmer JD, Thompson WF (1982) Cell 29:537–550
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983a) Nature 301:725–728
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983b) Theor Appl Genet 65:181–189
Schuster W, Brennicke A (1987) EMBO J 6:2857–2863
Scott NS, Timmis JN (1984) Theor Appl Genet 67:279–288
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Siculella L, Palmer JD (1988) Nucleic Acids Res 16:3787–3799
Stern DB, Lonsdale DM (1982) Nature 299:698–702
Stern DB, Palmer JD (1984) Proc Natl Acad Sci USA 81:1946–1950
Stern DB, Palmer JD (1986) Nucleic Acids Res 14:5651–5666
Stern DB, Palmer JD, Thompson WF, Lonsdale DM (1983) In: Goldberg RB (ed) Plant molecular biology. Liss, New York, pp 467–477
Strittmatter G, Kössel H (1984) Nucleic Acids Res 12:7633–7647
Timmis JN, Scott NS (1983) Nature 305:65–67
Ward BL, Anderson RS, Bendich AJ (1981) Cell 25:793–803
Wintz H, Grienenberger J-M, Weil J-H, Lonsdale DM (1988) Curr Genet 13:247–254
Zurawski G, Whitfield PR, Bottomley W (1986) Nucleic Acids Res 14:3975
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Nugent, J.M., Palmer, J.D. Location, identity, amount and serial entry of chloroplast DNA sequences in crucifer mitochondrial DNAs. Curr Genet 14, 501–509 (1988). https://doi.org/10.1007/BF00521276
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DOI: https://doi.org/10.1007/BF00521276