Summary
We have located the positions of the genes coding for the α, β and ε subunits of the ATPase complex on Spirodela oligorhiza chloroplast DNA by means of heterologous hybridization with Spinacia cpDNA fragments.
The overall cpDNA sequence organization of Petunia hybrida and Spirodela was compared. We hybridized well-characterized, cloned Spirodela cpDNA fragments with size fractionated Petunia cpDNA digested by Sall. It appears that the monocotyledonous Spirodela and the dicotyledonous Petunia cpDNA share a common sequence organization around their entire circumference. These observations, together with data reported in the literature, indicate a strikingly similar genetic organization of the chloroplast genome in widely divergent plants.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- ATPase = CF1 :
-
chloroplast coupling factor
- cp:
-
chloroplast
- kbp:
-
kilo base pairs
- LSU:
-
large subunit of ribulose-1,5-bisphosphate carboxylase
- BSA:
-
bovine serum albumine
- SDS:
-
sodium dodecylsulphate
- kD:
-
kilo dalton
- PVP:
-
polyvinylpyrrolidon
- SSC:
-
standard sodium saline citrate
- PS II:
-
photosystem II
References
Bedbrook JR, Kolodner R, Bogorad L (1976) Cell 11:739–749
Bedbrook JR, Link G, Coen DM, Bogorad L, Rich A (1978) Proc Natl Acad Sci USA 75:3060–3064
Bedbrook JR, Coen DM, Beaton AR, Bogorad L, Rich A (1979) J Biol Chem 254:905–910
Bedbrook JR, Beaton AR, Bogorad L, Coen DM, Dyer TA, Kolodner R (1980) In: Davies DR, Hopwood DA (eds) The plant genome. The John Innes Charity, pp 121–130
Birnboim NC, Doly J (1979) Nucleic Acids Res 7:1513–1523
Bovenberg WA, Kool AJ, Nijkamp HJJ (1981) Nucleic Acids Res 9:503–517
Crouse EJ, Schmitt JM, Bohnert HJ, Gordon K, Driesel AJ, Herrmann RG (1978) In: Akoyunoglou G, Argyroudi-Akoyunoglou JH (eds) Chloroplast development, Elsevier/North Holland, pp 565–572
Dagart M, Ehrlich SD (1979) Gene 6:23–28
Denhardt DT (1966) Biochem Biophys Res Comm 23:641–646
Driesel AJ, Crouse EJ, Gordon K, Bohnert HJ, Herrmann RG, Steinmetz A, Mubumbila M, Keller M, Burkhard G, Weil JH (1979) Gene 6:285–306
Driesel AJ, Speirs J, Bohnert HJ (1980) Biochem Biophys Acta 610:297–310
Dyer T, Bedbrook JR (1980) In: Leaver CJ (ed) Genome organization and expression in plants. Plenum Press, New York, pp 305–311
Edwards K, Kössel H (1981) Nucleic Acids Res 9:2853–2869
Fluhr R, Edelman M (1981) Nucleic Acids Res 9:6841–6853
Gordon KHJ, Crouse EJ, Bohnert HJ, Herrmann RG (1981) Theor Applied Genet 59:281–286
Groot GSP, van Harten-Loosbroek N (1981) Curr Genet 4:187–190
Herrmann RG, Possingham JV (1980) In: Reinert J (ed) Results and problems in cell differentiation, vol 10. Springer, Heidelberg Berlin New York, pp 45–96
Jeffreys AJ, Flavell RA (1977) Cell 12:429–439
Koch W, Edwards K, Kössel H (1981) Cell 25:203–213
Koller B, Delius H (1980) Mol Gen Genet 178:261–269
Kolodner R, Tewari KK (1979) Proc Natl Acad Sci USA 76:41–45
Kung SD, Zhu YS, Shen GF (1982) Theor Applied Genet 61:73–79
Link G (1981) Nucleic Acids Res 15:3681–3694
Mattoo AK, Pick U, Hoffmann-Falk H, Edelman M (1981) Proc Natl Acad Sci USA 78:1572–1576
Palmer JD (1982) Nucleic Acids Res 10:1593–1605
Palmer JD, Thompson WF (1981) Proc Natl Acad Sci USA 78:5533–5537
Palmer JD, Thompson WF (1982) Cell 29:537–550
Penefski HS (1977) J Biol Chem 252:2891–2899
Schwarz ZS, Kössel H (1979) Nature 279:520–522
Seyer P, Kowallik KV, Herrmann RG (1981) Curr Genet 3:189–204
Southern EM (1979) In: Colowick S, Kaplan N (eds) Methods in enzymology, vol 68. Academic Press, New York, pp 152–176
Takaiwa F, Sugiura M (1982) Eur J Biochem 124:13–19
Tohdoh N, Sugiura M (1982) Gene 17:213–218
van Ee JH, Man in 't Veld WA, Planta RJ (1980a) Plant Physiol 66:572–575
van Ee, Vos YJ, Planta RJ (1980b) Gene 12:191–200
van Ee JH, Vos YJ, Bohnert HJ, Planta RJ (1982) Plant Mol Biol 2:117–131
Weil HJ, Guillemaut P, Burkhard G, Canady J, Mubumbila M, Osorio ML, Keller M, Gloeckler R, Steinmetz A, Keith G, Heiser D, Crouse EJ (1981) In: Akoyunoglou G (ed) Photosynthesis. Proc Vth Intern Congress on Photosynthesis, Balaban Intern Sci Services, pp 777–786
Westhoff P, Nelson N, Bünemann H, Herrmann RG (1981) Curr Genet 4:109–120
Whitfeld PR, Bottomley W (1980) Biochem Intern 1:172–178
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de Heij, H.T., Lustig, H., Moeskops, DJ.M. et al. Chloroplast DNAs of Spinacia, Petunia and Spirodela have a similar gene organization. Current Genetics 7, 1–6 (1983). https://doi.org/10.1007/BF00365673
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00365673