Summary
A HPLC method using a fluorescence detector is described for the determination of halogenated benz(a)anthracenes (BAX, x=Cl or Br) in water. It consists of the following three procedures; (1) liquid-liquid extraction of BAX with benzene, (2) elimination of interfering compounds from the extracts by one dimensional dual-band thin layer chromatography, and (3) quantitative determination of BAX by HPLC equipped with a fluorescence detector. The recoveries of BAX from water samples through the entire analytical procedure amounted to more than 72%. The calibration curves for BAX were linear, e.g., with a range from 1 to 20 ng ml−1 for 7-chlorobenz(a)anthracene (BACl). The lower detection limit for BACl was 20 pg for an injection volume of 20 μl. This method has been applied to BAX in river water spiked with benz(a)anthracene [B(a)A] at 10 ng ml−1 after chlorination. B(a)A, BACl and BABr were found in the levels of 2.01, 0.16 and 0.13 or less ng ml−1, respectively.
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Mori, Y., Goto, S., Onodera, S. et al. Simultaneous determination of halogenated benz(a)anthracenes in water by HPLC with fluorometric detection. Fresenius J Anal Chem 345, 63–67 (1993). https://doi.org/10.1007/BF00323328
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DOI: https://doi.org/10.1007/BF00323328