Abstract
A 1887-nucleotide chloroplast-DNA region from Arabidopsis thaliana was analyzed. It contains the conserved genes psbA for the precursor of the D1 reaction-centre protein of photosystem II, trnH for tRNAHis, and rps19′ for the 6.8-kDa protein of the small ribosomal subunit. Northern hybridization and RNase protection experiments suggest co-transcription of a minor RNA fraction over the full lengths of psbA and the preceding trnK-UUU gene, but not including downstream trnH sequences. In front of the mapped 5′ end of the major 1.2-kb psbA transcript is a DNA region that shows the typical architecture of a psbA promoter, consisting of the prokaryotic-type ‘-35’ and ‘-10’ elements as well as the eukaryotic-type ‘TATA’ motif. The common 3′ end of psbA transcripts seems to be located immediately after a stem-loop structure downstream from the coding region.
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Communicated by H. Kössel
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Liere, K., Kestermann, M., Müller, U. et al. Identification and characterization of the Arabidopsis thaliana chloroplast DNA region containing the genes psbA, trnH and rps19′ . Curr Genet 28, 128–130 (1995). https://doi.org/10.1007/BF00315778
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DOI: https://doi.org/10.1007/BF00315778