Abstract
Chloroplasts are essential semiautonomous plant organelles responsible for photosynthesis, which generates sugars and oxygen vital for the entire biosphere. Additionally, chloroplasts regulate energy production, metabolite synthesis, and stress responses in plants and algae. Chloroplasts possess a notably complex RNA metabolism that includes RNA processing, editing, splicing, and regulation by various RNA-binding proteins. Highly purified chloroplasts, free of nuclear/cytoplasmic contaminants are desirable when studying chloroplast RNA metabolism. Here, we describe an efficient protocol to obtain highly purified chloroplasts for RNA analysis.
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Acknowledgements
Work in Dr. Hailing Jin’s laboratory was supported by grants from National Institute of Health (R01 GM093008), National Science Foundation (IOS-1557812), and United States Department of Agriculture (2019-70016-29067).
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Wang, H., Jin, H. (2021). Purification and Analysis of Chloroplast RNAs in Arabidopsis . In: Jin, H., Kaloshian, I. (eds) RNA Abundance Analysis . Methods in Molecular Biology, vol 2170. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0743-5_10
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DOI: https://doi.org/10.1007/978-1-0716-0743-5_10
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0743-5
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