Summary
Cell-free hemolymph of females of the spider, Cupiennius salei Keyserling, has been analyzed for its main inorganic and organic constituents.
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1.
The hemolymph pH is in the range 8.1–8.3, as measured in vivo by a micro glass electrode and in vitro by indicator paper. Total dry matter amounts to 76.9 mg/ml, 21.5 mg/ml of which can be extracted by an alcohol/ether mixture.
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2.
Quantitative analyses are summarized in Table 1. The sum of all components is slightly higher than total dry matter. Separate determinations of nitrogen and phosphate fractions are in good agreement with the sum totals. Soluble phosphate is entirely inorganic.
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3.
The major solute is protein (48 mg/ml), which at least partly consists of hemocyanin as judged from absorption spectra. The main blood sugar of Cupiennius is glucose, but two more components have been detected by thin layer chromatography.
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4.
The hemolymph composition of Cupiennius is of the same type as that of the scorpion, Heterometrus, of Limulus, and of Crustacea.
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Literatur
Andrews, P.: Über den Blutchemismus des Flußkrebses Orconectes limosus und seine Veränderung im Laufe des Jahres. Z. vergl. Physiol. 57, 7–43 (1967).
Bergmeyer, H. U.: Methoden der enzymatischen Analyse. Weinheim/Bergstraße: Verlag Chemie 1962.
Björnesjö, K. B.: Analysis of protein-bound serum polysaccharides with anthron reagent. Scand. J. clin. Lab. Invest. 7, 147–152 (1955).
Boyd, W. C.: Cross-reactivity of various hemocyanins with special reference to the blood proteins of the black widow spider. Biol. Bull. 73, 181–183 (1937).
Bruggen, E. F. J. van: Electron microscopy of hemocyanins. In: Physiology and biochemistry of haemocyanins (F. Ghiretti, ed.), p. 37–48. London and New York: Academic Press 1968.
Eden, A., Green, H. M.: Microdetermination of copper in biological material. Biochem. J. 34, 1202–1203 (1940).
Jacobs, S.: Determination of nitrogen in proteins by means of indanetrione hydrate. Nature (Lond.) 183, 262 (1959).
Lang, C. A.: Simple microdetermination of Kjeldahl nitrogen in biological materials. Analyt. Chem. 30, 1692–1694 (1958).
Lowry, O. H., Roberts, N. R., Leinen, K. Y., Wu, M. L., Farr, A. L.: The quantitative histochemistry of brain. I. Chemical methods. J. biol. Chem. 207, 1–17 (1954).
—, Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Maluf, N. S. R.: The blood of arthropods. Quart. Rev. Biol. 14, 149–191 (1939).
Martin, J. B., Doty, D. M.: Determination of inorganic phosphate. Modification of the isobutyl alcohol procedure. Analyt. Chem. 21, 965–967 (1949).
Mattenheimer, H.: Mikromethoden für das klinisch-chemische und biochemische Laboratorium. Berlin: Walter de Gruyter 1966.
McCrone, J. D.: Biochemical differentiation of the sibling black widow spiders, Latrodectus mactans and L. variolus. Psyche 74, 212–217 (1967).
Melchers, M.: Zur Biologie und zum Verhalten von Cupiennius salei (Keyserling), einer amerikanischen Ctenide. Zool. Jb., Abt. System., Ökol. u. Geogr. 91, 1–90 (1963).
Millot, J.: Contribution à l'histophysiologie des Aranéides. Bull. biol. France Belg., Suppl. 8, 1–238 (1926).
Mokrasch, L. C.: Analysis of hexose phosphates and sugar mixtures with anthrone reagent. J. biol. Chem. 208, 55–59 (1954).
Mueller, P.: Experiments on current flow and ionic movements in single myelinated nerve fibers. Exp. Cell Res. 5, 118–146 (1958).
Padmanabhanaidu, B.: Ionic composition of the blood and the blood volume of the scorpion Heterometrus fulvipes. Comp. Biochem. Physiol. 17, 157–166 (1966 a).
—: Influence of size, sex, pH and temperature on heart beat of the scorpion, Heterometrus fulvipes. Indian J. exp. Biol. 4, 206–208 (1966 b).
Prosser, C. L., Brown, F. A.: Comparative animal physiology. Philadelphia: W. B. Saunders 1961.
Rabaey, M., Verriest, G.: Etude comparative de l'hémolymphe de 33 espéces d'araignées par microélectrophorèse sur gèlose. Interêt systématique de cette méthode. Ann. Soc. Zool. Belg. 88, 373–383 (1957–58).
Ramsay, J. A., Brown, R. H., Croghan, P. C.: Electrometric titration of chloride in small volumes. J. exp. Biol. 32, 822–829 (1955).
Rathmeyer, W.: Neuromuscular transmission in a spider and the effect of calcium. Comp. Biochem. Physiol. 14, 673–687 (1965).
Sattler, L., Zerban, F. W.: New spray reagents for paper chromatography of reducing sugars. Analyt. Chem. 24, 1862 (1952).
Stackelberg, W. v.: Dissertation (in Vorbereitung).
Trevelyan, W. E., Procter, D. P., Harrison, J. S.: Detection of sugars on paper chromatograms. Nature (Lond.) 166, 444–445 (1950).
Vomhof, D. W., Tucker, T. C.: The separation of simple sugars by cellulose thin-layer chromatography. J. Chromatogr. 17, 300–306 (1965).
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Wir danken Herrn Prof. H. Autrum für die Förderung der Arbeit, Herrn W. Kuschinsky für seine Hilfe bei den Kaliumbestimmungen und der Deutschen Forschungsgemeinschaft für ihre Leihgaben.
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Loewe, R., Linzen, B. & von Stackelberg, W. Die gelösten Stoffe in der Hämolymphe einer Spinne, Cupiennius salei Keyserling. Z. Vergl. Physiol. 66, 27–34 (1970). https://doi.org/10.1007/BF00297796
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DOI: https://doi.org/10.1007/BF00297796