Summary
The hydrolysis of glucose-6-phospate in the digestive gland of the crab Carcinus maenas is carried out by an aspecific phosphatase. This enzyme possesses the following features: (1) insensitivity to acid treatment; (2) absence of inhibition when exposed to citrate at low pH; (3) similar affinity for G6P as the acid phosphatase for Na-β-glycerophosphate (K m 2.3 and 2.0 mM, respectively). Glucose-6-phosphate and Na-β-glycerophate hydrolysis reactions seem to be catalysed by the same enzyme, since both activities exhibit the same distribution in a subcellular fractionation of the gland. Furthermore, as these activities are principally recovered in the subcellular fraction enriched in calcospherites (or calcium phosphate granules), it is proposed that the aspecific G6P-phosphohydrolase could play a major role in the formation of these granules. The phosphorylation of glucose is made by two “low K m” hexokinases (230 and 64 μM, respectively). As their level of activity shows significant changes over the moult cycle, these enzymes could be considered as having a regulatory role in the storage of glucose in the digestive gland.
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Abbreviations
- Acid Pase:
-
aspecific acid phosphatase
- ATP:
-
adenosine triphosphate
- DTT:
-
dithiothreitol
- EDTA:
-
ethylenediaminetetra-acetate
- G:
-
calcium phosphate granules fraction
- G6P:
-
glucose-6-phosphate
- G6Pase:
-
hepatic glucose-6-phosphatase
- G6PDH:
-
glucose-6-phosphate dehydrogenase
- K m :
-
Michaelis-Menten constant
- MI:
-
mitochondria and intermediate postmitochondrial particles
- N:
-
nuclei fraction
- NADH:
-
nicotineamide adenine dinucleotide
- P:
-
microsome fraction
- Pi:
-
inorganic phosphate
- PMSF:
-
phenylmethylsulphonylfluoride
- STI:
-
soybean trypsin inhibitor
- βglyP:
-
Na-β-glycerophosphate
- T1,2,3 :
-
transport protein 1,2,3
- TCA:
-
trichloroacetic acid
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Loret, S.M., Devos, P.E. Hydrolysis of G6P by a microsomal aspecific phosphatase and glucose phosphorylation by a low K m hexokinase in the digestive gland of the crab Carcinus maenas: variations during the moult cycle. J Comp Physiol B 162, 651–657 (1992). https://doi.org/10.1007/BF00296647
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DOI: https://doi.org/10.1007/BF00296647