Abstract
Leaves of three different sizes excised from 14, 21, 28 and 35-day-old seedlings of Cucumis melo were cultured on a MS medium supplemented with a range and combination of growth regulators. Maximum shoot differentiation from the leaf explants occurred in the combined presence of BAP and 2iP at equimolar concentration of 1 μM. Regeneration potential of leaves declined with increasing size of the leaves and the age of the donor seedlings. For elongation the shoots were transferred to MS+BAP [1 μM]. Such shoots were rooted with 75% frequency on MS+IAA [0.5 μM]. The plants have been established in pots.
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Abbreviations
- BM:
-
Basal Medium
- MS:
-
Murashige and Skoog
- BAP:
-
6-Benzyl amino-purine
- 2iP:
-
6- V, V -dimethylallylamino purine
- IAA:
-
Indole-3-acetic acid
References
Blackman W J, Reynolch B D, Postek C E [1981] HortScience 16: 451
Bouabdallah L, Branchard M [1986] Z. Pflanzenzücht, 96: 82–85
Halder T, Gadgil V N [1982] Ind J Exp Biol, 20: 780–782
Kathal R, Bhatnagar S P, Bhojwani S S [1986] J Plant Physiol, 126: 59–62
Murashige T, Skoog F [1962] Physiol Plant, 15: 473 - 497
Malepszy S, Nadolska - Orczyk A [1983] J Plant Physiol, 111: 273–276
Moreno V, Zubeldia L, Roig L A [1984] Plant Sci Lett, 34: 195 - 201
Moreno V, Garcia-Sogo M, Granell I, Garcia-Sogo B, Roig L A [1985] Plant Cell Tissue Organ Cult, 5: 139–146
Orczyk W, Malepszy S [1985] Plant Cell Rep, 4: 269 - 273
Oridate T, Oosawa K [1986] Jap J Breed, 36: 424 - 428
Orts M C [1987] HortScience, 22: 666
Trulson A J, Shahin E A [1986] Plant Sci Lett, 47: 35 - 43
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Communicated by I. K. Vasil
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Kathal, R., Bhatnagar, S.P. & Bhojwani, S.S. Regeneration of plants from leaf explants of Cucumis melo cv. Pusa Sharbati. Plant Cell Reports 7, 449–451 (1988). https://doi.org/10.1007/BF00269535
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DOI: https://doi.org/10.1007/BF00269535