Abstract
Isolated microspores of Chinese cabbage (Brassica campestris ssp. pekinensis) were incubated in modified NN medium containing 10% sucrose in darkness at 33°C for one day followed by culture at 25°C. After 14 days of culture, microspores developed into embryos ranging from globular to cotyledonary stage. Plants were regenerated after transfer of embryos to medium containing 3% sucrose and no plant growth regulators.
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Abbreviations
- NN:
-
Nitsch and Nitsch
- MS:
-
Murashige and Skoog
- NAA:
-
naphthaleneacetic acid
- BA:
-
6-benzylaminopurine
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Communicated by E. D. Earle
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Sato, T., Nishio, T. & Hirai, M. Plant regeneration from isolated microspore cultures of Chinese cabbage (Brassica campestris spp. pekinensis). Plant Cell Reports 8, 486–488 (1989). https://doi.org/10.1007/BF00269055
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DOI: https://doi.org/10.1007/BF00269055