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Brassica rapa L. ssp. chinensis Isolated Microspore Culture Protocol

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2288))

Abstract

Culture of isolated microspores is a widely used method to obtain haploid and doubled haploid plants for many crop species. This protocol describes the steps necessary to obtain a large number of microspore derived embryos for pakchoi (Brassica rapa L. ssp. chinensis) and zicaitai (Brassica rapa L. ssp. сhinensis Hanelt var. purpuraria Kitam).

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Authors and Affiliations

  1. Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center, VNIISSOK, Russian Federation

    Daria Shumilina, Elena Kozar, Olga Chichvarina, Ksenia Korottseva & Elena Domblides

Authors
  1. Daria Shumilina
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  2. Elena Kozar
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  3. Olga Chichvarina
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  4. Ksenia Korottseva
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  5. Elena Domblides
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Editor information

Editors and Affiliations

  1. Cell Biology Group - COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Jose M. Segui-Simarro

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© 2021 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Shumilina, D., Kozar, E., Chichvarina, O., Korottseva, K., Domblides, E. (2021). Brassica rapa L. ssp. chinensis Isolated Microspore Culture Protocol. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2288. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1335-1_9

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  • DOI: https://doi.org/10.1007/978-1-0716-1335-1_9

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1334-4

  • Online ISBN: 978-1-0716-1335-1

  • eBook Packages: Springer Protocols

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