Abstract
Investigations on the absorption of shortchain fatty acids across ruminal epithelium of sheep were performed both in vitro (Ussing chamber technique, using propionic acid representatively for shortchain fatty acids) and in vivo (washed, isolated reticulorumen). A pH-induced, nearly tenfold increase in the concentration of undissociated propionate led to an only twofold increase in mucosal-to-serosal flux of propionate (in vitro). Neither amiloride (1 mmol·l-1, in vitro) nor theophylline (10 mmol·l-1, in vivo), inhibitors of the ruminal Na+/H+ exchanger, exerted any significant influence on propionate fluxes or short-chain fatty acids absorption, respectively. Total replacement of luminal Na+ (by choline) did not alter short-chain fatty acids absorption (in vivo). Mucosal 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (0.1 mmol·l-1) or mucosal nitrate (40 mmol·l-1) markedly reduced propionate net flux (in vitro). Increasing mucosal Cl- concentration brought about a significant drop in mucosal-to-serosal flux of propionate (in vitro) and in short-chain fatty acids net absorption (in vivo), respectively. The results obtained suggest that short-chain fatty acids are absorbed both as anions and as undissociated acids across ruminal epithelium of sheep. It is concluded that short-chain fatty acids anions either compete with Cl- for binding sites at a common anion-exchange mechanism or that they are absorbed by an short-chain fatty acids anion/HCO -3 exchanger indirectly coupled to a Cl-/HCO -3 exchanger via intracellular bicarbonate.
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Abbreviations
- DIDS :
-
4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid
- DMSO :
-
dimethylsulfoxide
- G t :
-
tissue conductance
- HSCFA :
-
protonated
- SCFA:
-
i.e. undissociated form
- J ms :
-
mucosal-to-serosal flux
- J sm :
-
serosal-to-mucosal flux
- J net :
-
net flux
- I sc :
-
short-circuit current
- MOPS :
-
(3-[N-morpholino]propanesulfonic acid)
- mu :
-
mucosal
- Prop :
-
Propionate
- SCFA - SCFA:
-
anions, i.e. dissociated form
- SCFA :
-
short-chain fatty acids
- SEM :
-
standard error of mean
References
Aafjes JH (1967) The disappearance of volatile fatty acids through the rumen wall. J Anim Physiol Anim Nutr 22: 69–75
Aronson PS, Seifter J (1984) Cl--transport via anion exchange. Fed Proc 43: 2483–2484
Ash RW, Dobson A (1963) The effect of absorption on the acidity of rumen contents. J Physiol (Lond) 169: 39–61
Bergman EN (1990) Energy contributions of volatile fatty acids from the gastrointestinal tract in various species. Physiol Rev 70: 567–590
Bugaut M (1987) Occurrence, absorption and metabolism of short chain fatty acids in the digestive tract of mammals. Comp Biochem Physiol 86B: 439–472
Care AD, Brown RC, Farrar AR, Pickard DW (1984) Magnesium absorption from the digestive tract of sheep. Q J Exp Physiol 69: 577–587
Chien WJ, Stevens CE (1972) Coupled active transport of Na and Cl across forestomach epithelium. Am J Physiol 223:997–1003
Engelhardt W von, Sallmann HP (1972) Resorption und Sekretion im Pansen des Guanakos (Lama guanacoe). Zbl Vet Med A 19: 117–132
Engelhardt W von (1995) Absorption of short-chain fatty acids from the large intestine. In: Cummings JH et al. (eds) Physiological and clinical aspects of short-chain fatty acids. Cambridge University Press, Cambridge, pp 149–170
Engelhardt W von, Burmester M, Hansen K, Becker G, Rechkemmer G (1993) Effects of amiloride and ouabain on short-chain fatty acid transport in guinea-pig large intestine. J Physiol (Lond) 460: 455–466
Gäbel G, Vogler S, Martens H (1991) Short-chain fatty acids and CO2 as regulators of Na+ and Cl- absorption in isolated sheep rumen mucosa. J Comp Physiol B 161: 419–426
Gäbel G, Butter H, Vogler S, Martens H (1992) Effect of theophyl-line, forskolin and prostaglandin on Na+ transport across sheep rumen epithelium. Z Gastroenterol 30: 224
Harig JM, Soergel KH, Barry JA, Ramaswamy K (1991) Transport of propionate by human ilcal brush-border membrane vesicles. Am J Physiol 260: G776-G782
Holtug K, Rasmussen HS, Mortensen PB (1992) An in vitro study of short-chain fatty acid concentrations, production and absorption in pig (Sus scrofa) colon. Comp Biochem Physiol 103A: 189–197
Jennings ML (1992) Cellular anion transport. In: Seldin DW, Giebisch G (eds) The kidney: physiology and pathophysiology. Raven Press, New York, pp 113–145
John N, Oswald R, Gäbel G (1995) Transport of short chain fatty acids (SCFA) across sheep rumen epithelium: influence of the epithelial metabolism. Ital J Gastroenterol 27: 147
Macfarlane GT, Cummings JH (1991) The colonic flora, fermentation, and large bowel digestive function. In: Phillips SF et al. (eds) Large intestine: physiology, pathophysiology, and disease. Raven Press, New York, pp 51–92
Martens H, Gäbel G, Strozyk B (1991) Mechanism of electrically silent Na and Cl transport across the rumen epithelium of sheep. Exp Physiol 76: 103–114
Martens H, Henseleit M, Gäbel G (1989) Electrogenic, amiloride-insensitive Na-transport by sheep rumen epithelium is inhibited by serosal theophylline. Pflügers Arch 413 [Suppl 1]: R4
Mascolo N, Rajendran VH, Binder HJ (1991) Mechanisms of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon. Gastroenterology 101: 331–338
Rajendran VM, Binder HJ (1994) Short chain fatty acid stimulation of electroneutral Na−Cl absorption: role of apical SCFA-HCO3 and SCFA-Cl exchanges. In: Binder HJ et al. (eds) Short chain fatty acids. Kluwer Academic Publishers, Dordrecht Boston London, pp 104–116
Rechkemmer G (1994) In vitro studies of short chain fatty acid transport with intact tissue. In: Binder HJ et al. (eds) Short chain fatty acids. Kluwer Academic Publishers, Dordrecht Boston London, pp 83–92
Rechkemmer G, Engelhardt W (1988) Concentration- and pH-dependence of short-chain fatty acid absorption in the proximal and distal colon of guinea pig (Cavia porcellus). Comp Biochem Physiol A 91: 659–663
Schested J, Diernæs, Møller PD, Skadhauge E (1996) Transport of sodium across the isolated bovine rumen epithelium: interaction with short-chain fatty acids, chloride and bicarbonate. Exp Physiol 81: 79–94
Soergel KH, Harig JM, Loo FD, Ramaswamy K, Wood CM (1989) Colonic fermentation and absorption of SCFA in man. Acta Vet Scand [Suppl] 86: 107–115
Stevens CE (1970) Fatty acid transport through the rumen epithelium. In: Phillipson AT (ed) Physiology of digestion and metabolism in the ruminant. Oriel Press, Newcastle upon Tyne, pp 101–112
Stevens CE, Stettler BK (1966) Factors affecting the transport of volatile fatty acids across rument epithelium. Am J Physiol 210: 365–372
Thorlacius SO, Lodge GA (1973) Absorption of steam-volatile fatty acids from the rumen of the cow as influenced by diet, buffers, and pH. Can J Anim Sci 53: 279–288
Titus A, Ahearn GA (1988) Short-chain fatty acid transport in the intestine of a herbivorous teleost. J Exp Biol 135: 77–94
Weigand E, Young JW, McGilliard AD (1972) Extent of propionate metabolism during absorption from the bovine ruminoreticulum. Biochem J 126: 201–209
Wolffram S, Frischknecht R, Scharrer E (1989) Influence of theophylline on the electrical potential difference and ion fluxes (Na, Cl, K) across the isolated rumen epithelium of sheep. J Vet Med A 36: 755–762
Würmli R, Wolffram S, Scharrer E (1987) Inhibition of chloride absorption from the sheep rumen by nitrate. J Vet Med A 34: 476–479
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Kramer, T., Michelberger, T., Gürtler, H. et al. Absorption of short-chain fatty acids across ruminal epithelium of sheep. J Comp Physiol B 166, 262–269 (1996). https://doi.org/10.1007/BF00262870
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DOI: https://doi.org/10.1007/BF00262870