Abstract
The possibility to cultivate Lactococcus lactis in aqueous polymer two-phase system has been investigated. The phase system was made up of poly(ethylene imine) and (hydroxyethyl) cellulose. Long lag phases were needed for the microorganism to adapt to the polymer rich media. Cells favoured the (hydroxyethyl)cellulose rich top phase or they accumulated at the interface, while lactic acid showed affinity for the poly(ethylene imine) rich phase.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Abbreviations
- PEG:
-
poly(ethylene glycol)
- PEI:
-
poly(ethylene imine)
- HEC:
-
(hydroxyethyl)cellulose
References
Albertsson, P.Å. (1986). Partition of Cell particles and Macromolecules. Wiley Interscience, New York.
Andersson, E. and Hahn-Hägerdal, B. (1990). Appl. Microbiol. Biotechnol. (1988). 29, 329–33.
Dissing, U. and Mattiasson, B. (1993). Biotechnol. Appl. Biochem. 17, 15–21.
Hano, T., Matsumoto, M., Uenoyama, S., Ohtake, T., Kawano, Y and Miura, S. (1993). Bioseparation 3, 321–326.
Hjörleifsdottir, S., Holst, O. and Mattiasson, B. (1991). Bioprocess Engineering 6, 29–34.
Larsson, M., Arasaratnam, V and Mattiasson, B. (1988). J.Microb. Biotechnol. 3, 22–29
Luedeking, R. and Piret, E.L. (1959). J. Biochem. Microbiol. Technol. Eng. 1, 359–364.
Lukovkin, G.M., Pshezhetsky, V.S. and Murtazaeva, G.A. (1973). Eur. Polymer. J. 9, 559–565.
Mattiasson, B. and Holst, O. eds. (1991). Extractive Bioconversions. Marcel Dekker Inc. New York.
Reuben, J (1984). Macromolecules 17, 156–161.
Seevaratnam, S., Holst, O. and Mattiasson, B. (1991). Bioprocess Engineering 6, 35–41.
Schöller, C., Chaudhuri, J.B. and Pyle, D.L. (1993.) Biotechnol. Bioeng. 42, 50–58.
Srivastava, A., Roychoudhury, P.K. and Sahai, V (1992). Biotechnol. Bioeng. 39, 607–613.
Treweek, G. P. and Morgan, J.J. (1977). J. Colloid Interface Sci. 60, 258–273.
Yabannavar, V.M. and Wang, D.I.C. (1991). Biotechnol. Bioeng. 37, 1095–1100.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dissing, U., Mattiasson, B. Cultivation of Lactococcus lactis in a polyelectrolyte-neutral polymer aqueous two-phase system. Biotechnol Lett 16, 333–338 (1994). https://doi.org/10.1007/BF00245046
Issue Date:
DOI: https://doi.org/10.1007/BF00245046