Summary
Friable calli were induced on leaf segments of Saintpaulia ionantha Wendl. on B5 medium containing 1 mg l−1 2,4-D and 2 g l−1 casein hydrolysate. Cell suspension cultures were readily established from these friable calli and protoplasts could be isolated from the cells with yields of 1–3×107/g f. wt.. By culturing in 0.1 % gellan gum-solidified B5 medium supplemented with 1 mg l−1 2,4-D and 0.1 M each of sucrose and mannitol at a density of 1×105/ml, the protoplasts divided within 6 days and formed macro-colonies after 2 months of culture. Shoot regeneration from protoplast-derived calli was obtained by sequential treatment of the calli with plant growth regulators: initially with 1 mg l−1 each of NAA and BA for 2 months followed by 0.01 mg l−1 NAA and 5 mg l−1 BA for 4 months. Regenerated plants were established after rooting of the shoots on half-strength MS medium, and successfully transferred to the greenhouse. The regenerated plants grew into flowering stage and showed the same phenotype as the parent plant.
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Abbreviations
- BA:
-
benzyladenine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- FDA:
-
fluorescein diacetate
- f. wt:
-
fresh weight
- MES:
-
2-(N-morpholino)-ethanesulfonic acid
- MS:
-
Murashige and Skoog (1962)
- NAA:
-
α-naphthaleneacetic acid
- PE:
-
plating efficiency
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Communicated by J.M. Widholm
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Hoshino, Y., Nakano, M. & Mii, M. Plant regeneration from cell suspension-derived protoplasts of Saintpaulia ionantha Wendl. Plant Cell Reports 14, 341–344 (1995). https://doi.org/10.1007/BF00238593
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DOI: https://doi.org/10.1007/BF00238593