Summary
The addition of thidiazuron (TDZ) to MS salts and vitamins, instead of benzylaminopurine (BAP) increased both the culture weight and the proportion of ash embryo hypocotyl explants that produced adventitious shoots. The concentration of BAP, but not that of TDZ also affected these parameters. Addition of 1-naphthalene acetic acid reduced culture performance, indolebutyric acid (IBA) was beneficial, and 2,4-dichlorophenoxyacetic acid had no effect. During both 1990 and 1991, rates of regeneration declined as the growth season progressed. Adventitious shoots, which were also obtained from hypocotyls from dried seeds, were established as proliferating shoot cultures following transfer to DKW medium with 5.0 mg l−1 BAP, and the resulting shoots were rooted in half-strength Woody Plant medium with 1.0 mg l−1 IBA.
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References
Bolyard MG, Srinivasan C, Cheng J, Sticklen M (1991) HortSci 26:1554–1555
Chalupa V (1990) Biol Plant 32:332–338
Cox DR, Snell EJ (1989) Analysis of binary data. 2nd Edition. Chapman & Hall, London.
Dolcet-Sanjuan R, Mok DWS, Mok MC (1991) Plant Cell Rep 10:240–242
Driver JA, Kuniyuki AH (1984) HortSci 19:507–509
Fujino Y (1980) Biometrika 67:677–681
Hammatt (1992) World J Microbiol Biotech (in press)
Hammatt N, Ridout MS (1992) Plant Cell, Tissue and Organ Cult (in press)
Lloyd G, McCown B (1980) Comb Proc Intl Plant Prop Soc 30:421–427
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Preece JE, Imel MR (1991) Scientia Hortic 48:159–170
Preece JE, Christ PH, Ensenberger L, Zhao J-L (1987) Comb Proc Intl Plant Prop Soc 37:366–372
Preece JE, Zhao J-L, Kung FH (1989) HortSci 24:377–380
Wallin A, Johansson L (1989) J Plant Physiol 135:565–570
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Communicated by M.R. Davey
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Tabrett, A.M., Hammatt, N. Regeneration of shoots from embryo hypocotyls of common ash (Fraxinus excelsior). Plant Cell Reports 11, 514–518 (1992). https://doi.org/10.1007/BF00236267
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DOI: https://doi.org/10.1007/BF00236267