Summary
Chemiluminescence, indomethacin-sensitive suppression, and adherent cell cytotoxicity were measured in peripheral blood mononuclear cells (PBMC) from normal subjects and solid tumor cancer patients. These functions were found to be differentially affected by malignant disease. In cancer patients with disseminated disease, indomethacin-sensitive suppression and chemiluminescence emission were increased to a level significantly higher than normal without a concurrent increase in adherent cell cytotoxic function. In cancer patients with at most minimum residual diseases, the levels of chemiluminescence, indomethacin-sensitive suppression, and adherent cell cytotoxicity found were comparable to those of the normal study population. In vitro stimulation of cells from patients with disseminated disease by phorbol myristic acetate (PMA) increased chemiluminescence overcame the suppressive effects of indomethacin-sensitive suppressor cells, and increased adherent cell cytotoxicity; in cells from patients with at most minimum residual disease, PMA increased chemiluminescence and cytotoxicity without influencing the activity of indomethacin-sensitive suppressor cells. Vaccination of lung cancer patients with Freund's complete adjuvant or Freund's complete adjuvant plus tumor antigen extracts led to increased levels of chemiluminescence and increased levels of adherent cell cytotoxicity without altering indomethacin-sensitive regulatory cell function.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Abb JB, Bayliss J, Deinhardt F (1979) Lymphocyte activation by the tumor promoting agent, 12–0-tetradecanoylphorbol-13-acetate (TPA). J Immunol 122:1639
Allen RC (1977) Evaluation of serum opsonic capacity by quantitating the initial chemiluminescent response from phagocytizing polymorphonuclear leukocytes. Infect Immun 15:828
Allen RC (1980a) Chemiluminescence: An approach to the study of the humoral-phagocyte axis in host defense against infection. In: Peng CT, Horrocks DL, Alpen EL (eds) Liquid scintillation counting: Recent applications and developments. Academic Press, New York, p 377
Allen RC (1980b) Free-radical production by reticuloendothelial cells. In: Sbarra AJ, Strauss R (eds) The reticuloendothelial system, vol 2. Plenum, New York, p 309
Allen RC, Lint TF (1979) Correlation of chemiluminescence to microbicidal metabolic responses from polymorphonuclear leukocytes: a study of in vitro aging. In: Proceedings of the International Symposium on Analytical Application of Bioluminescence and Chemiluminescence. State Printing and Publishing, Westlake Village, CA, p 589
Allen RC, Stjernholm RL, Reed MA, Harper TB III, Gupta S, Steele RH, Waring WW (1977) Correlation of metabolic and chemiluminescent responses of granulocytes from three female siblings with chronic granulomatous disease. J Infect Dis 136:510–518
Bankhurst AD, Hastain E, Goodwin JS, Peake GT (1981) The nature of the prostaglandin-producing mononuclear cell in human peripheral blood. J Lab Clin Med 97:179
Braun DP, Harris JE (1982) Relationship of arachidonic acid metabolism and indomethacin-sensitive immunoregulatory function in peripheral blood mononuclear cells of disseminated solid tumor cancer patients. Proc Am Assoc Cancer Res 23:262
Braun DP, Mokyr MB, Dray S (1978) Generation of anti-MOPC-315 cytotoxicity in uneducated or in vitro-educated spleen cells from normal or MOPC-315 tumor-bearing mice pretreated in vivo with BCG. Cancer Res 38:1626
Braun DP, Cobleigh MA, Harris JE (1980) Multiple concurrent immunoregulatory defects in cancer patients with depressed PHA-induced lymphocyte DNA synthesis. Clin Immunol Immunopathol 17:89–101
Braun DP, Harris JE, Maximovich S, Marder R, Lint TF (1982) Chemiluminescence in peripheral blood mononuclear cells of solid tumor cancer patients. Cancer Immunol Immunother 12:31
Broder S, Humphrey R, Durm M, Blackman M, Meade B, Goldman C, Strober W, Waldmann R (1975) Impaired synthesis of polyclonal (non-paraprotein) immunoglobulin by circulating lymphocytes from patients with multiple myeloma. N Engl J Med 293:887
Catalona WJ, Taylor SPT, Rabson AS, Cretian PB (1972) A method for dinitrochlorobenzyne contact sensitization. N Engl J Med 286:399
Cowing C, Schwartz BD, Dickler HB (1978) Macrophage Ia antigens. I. Macrophage populations differ in their expression of Ia antigens. J Immunol 120:378
Freuhauf JP, Bonnard GD, Herberman RB (1981) The effect of lentinan on lymphokine production. Proc Am Assoc Cancer Res 22:275
Grebner JV, Mills EL, Gray BH, Quie PG (1977) Comparison of phagocytic and chemiluminescence response of human polymorphonuclear neutrophils. J Lab Clin Med 89:153
Hopper KE, Wood RP, Nelson DS (1979) Macrophage heterogeneity. Vox Sang 36:257
Jerrells TR, Dean JH, Richardson GL, McCoy JL, Herberman RB (1978) Role of suppressor cells in depression of in vitro lymphoproliferative responses of lung cancer and breast cancer patients. J Natl Cancer Inst 61:1001
Jerrells TR, Dean JH, Richardson GL, Cannon GB, Herberman RB (1979) Increased monocyte-mediated cytostatis of lymphoid cell lines in breast and lung cancer patients. Int J Cancer 23:768
Johnson RB Jr, Lehmeyer JE, Guthrie LA (1976) Generation of superoxide anion and chemiluminescence by human monocyte during phagocytosis and on contact with surface bound immunoglobulin G. J Exp Med 143:1551
Mantovani A, Polentarutti N, Peri G, Shavit ZB, Vecchi A, Bolis G, Mangioni C (1980) Cytotoxicity on tumor cells of peripheral blood monocytes and tumor-associated macrophages in patients with ascites ovarian tumors. J Natl Cancer Inst 64:1307
Meltzer MS, Stevenson M (1978) Macrophage function in tumor-bearing mice: Dissociation of phagocytic and chemotactic responsiveness. Cell Immunol 35:99
Mokry MB, Bennett JA, Braun DP, Hengst JCD, Mitchell MS, Dray S (1980) Opposite effects of different strains or batches of the same strain of BCG on the in vitro generation of syngeneic and allogeneic anti-tumor cytotoxicity. J Natl Cancer Inst 64:339
Nathan CF, Bruckner LH, Silverstein SC, Cohn ZA (1979) Extracellular cytolysis by activated macrophages and granulocytes. J Exp Med 149:84
Nelson RD, Mills EL, Simmons RL, Quie PG (1976) Chemiluminescence response of phagocytizing human monocytes. Infect Immun 14:12
Norris DA, Morris RM, Sanderson RJ, Kohler PF (1979) Isolation of functional subsets of human peripheral blood monocytes. J Immunol 123:166
Oehler JR, Herberman RB, Holden HT (1978) Modulation of immunity by macrophages. Pharmacol Ther 2:551
Rice L, Laughter AH, Twomey JJ (1979) Three suppressor systems in human blood that modulate lymphoproliferation. J Immunol 122:991
Samak R, Israel L, Edelstein R (1980) Influence of tumor burden, tumor removal, immune stimulation, plasmapheresis on monocyte mobilization in cancer patients. In: Friedman H (ed) Proceedings of the Eight International Congress of the Reticuloendothelial Society. Plenum, New York, p 411
Schleupner CJ, Glasgow LA (1978) Peritoneal macrophage activation indicated by enhanced chemiluminescence. Infect Immun 21:886
Sheagren HN, Block HB, Wolf SM (1967) Reticuloendothelial system phagocytic function in patients with Hodgkin's disease. J Clin Invest 46:855
Siegel BV (1976) The macrophage and cancer J Reticuloendothel Soc 20:219
Sohnle PG, Collins-Lech C (1979) Differentiation of the effects of preincubation and indomethacin on lymphocyte transformation. Clin Immunol Immunopathol 13:47
Stewart T, Hollinshead A, Harris JE, Raman S, Belanger R, Crepeau A, Crook AF, Hirte WE, Hooper D, Klaasen DJ, Rapp EF, Sachs HJ (1979) Specific active immunochemotherapy in lung cancer: A survival study. Recent Results Cancer Res 68:278
Vosixa G, Thies J (1979a) Indomethacin-sensitive suppressor cells in the diagnosis of immunoincompetence. Proc Am Assoc Cancer Res 20:101
Vosixa G, Thies J (1979b) Effect of indomethacin on blastogenesis of lymphocytes from cancer patients: Differentiation of patient types. Clin Immunol Immunopathol 13:30
Weidemann MJ, Peskar BA, Wrogemann K, Rietschel ETH, Staudinger H, Fischer H Prostaglandin and thromboxane synthesis in a pure macrophage population and the inhibition by E-type prostaglandins of chemiluminescence. FEBS Lett 89:136
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Braun, D.P., De Boer, K.P. & Harris, J.E. Chemiluminescence, suppression and cytotoxicity in peripheral blood mononuclear cells from solid tumor cancer patients. Cancer Immunol Immunother 14, 86–91 (1982). https://doi.org/10.1007/BF00200173
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00200173