The effects of ROS on functional properties (cytotoxic activity, antibody-producing activity, TNFα synthesis, and free cytosol calcium level), membrane structure (by expression of some surface markers), and apoptosis of lymphocytic cells were estimated in the peripheral blood of healthy volunteers. 1O2, \( {\mathrm{O}}_2^{\underset{\_}{\bullet }} \), OH•, and H2O2 mostly suppressed cytotoxic activity of lymphocytes against Ehrlich ascites carcinoma cells and inhibited IgG synthesis and expression of receptors and surface markers (Fc receptors, CD3, CD19, and CD56). The exposure of lymphocytes to H2O2 (10—6 M), 1O2, and OH• was followed by an increase in the level of a secondary messenger, intracellular calcium, in comparison with non-exposed cells. The presence of exogenous calcium in the medium for lymphocyte suspending induced an increase in the number of cells at early and late stages of apoptosis 6 h after exposure to H2O2 and 1O2 in comparison with lymphocytes incubated in Ca2+-free medium.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Kalaeva EA, Artyukhov VG, Kalaev VN. Theoretical Basis and Practical Application of Mathematical Statistics in Biological Experiments and Education. Voronezh, 2016. Russian.
Kobliakov VA. Mechanisms of tumor promotion by reactive oxygen species. Biochemistry (Moscow). 2010;75(6):675-685.
Koval’chuk LV, Ignat’eva GA, Gankovskaya LV. Immunology. Workshop. Moscow, 2010. Russian.
Nadeev AD, Zinchenko VP, Avdonin PV, Goncharov NV. Toxic and signal properties of active forms of oxigen. Toksikol. Vestn. 2014;(2):22-27. Russian.
Novikov PD, Konevalova NYu, Titova ND. The principles of evaluation of immune status and diagnostic of immunodeficiency diseases. Immunopatol., Allergol., Infektol. 2005;(2):8-22.
Novikov PD, Novikov DK. Comparative evaluation of contemporary methods of lymphocytes immunophenotyping. Immunopatol., Allergol., Infektol. 2000;(1):58-62.
Savitskaya MA, Onishchenko GE. Mechanisms of apoptosis. Biochemistry (Moscow). 2015;80(11):1393-1405.
Sanina EV, Slonimskaya EM, Kondakova IV, Garbukov EYu. Antioxidant system and lipid peroxidation in patients with and breast cancer precancer pathologies. Ross. Onkol. Zh. 2001;(1):20-22. Russian.
Tkachuk VA, Tyurin-Kuz’min PA, Belousov VV, Vorotnikov AV. Hydrogen peroxide as a new secondary transmitter. Biol. Membrany. 2012;29(1-2):21-37. Russian.
Khaitov RM, Ignatieva GA, Sidorovich IG. Immunology. Moscow, 2000. Russian.
Shpakova AP, Pavlova KS, Bulycheva TI. MTT-colorimetric method for measuring cytotoxic activity of natural killer cells. Klin. Lab. Diagnost. 2000;(2):20-23. Russian.
Feske S, Skolnik E.Y, Prakriya M. Ion channels and transporters in lymphocyte function and immunity. Nat. Rev. Immunol. 2012;12(7):532-547.
Hirst RA, Harrison C, Hirota K, Lambert DG. Measurement of [Ca2+]i in whole cell suspensions using fura-2. Methods Mol. Biol. 2005;312:37-45.
Oxidative Stress Biomarkers and Antioxidant Protocols. Armstrong D, ed. Gainesville, 2002.
Richter K, Kietzmann T. Reactive oxygen species and fibrosis: further evidence of a significant liaison. Cell Tissue Res. 2016;365(3):591-605.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 10, pp. 475-481, October, 2018
Rights and permissions
About this article
Cite this article
Nakvasina, M.A., Popova, L.I., Lidokhova, O.V. et al. Modulation of Structural and Functional Properties of Human Lymphocytes by Reactive Oxygen Species. Bull Exp Biol Med 166, 481–486 (2019). https://doi.org/10.1007/s10517-019-04377-4
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-019-04377-4