Summary
Rhodococcus rhodochrous PA-34 isolated from soil as a propionitrile-utilizing microorganism, hydrolysed several α-aminonitriles to optically active amino acids. The hydrolysis of α-aminonitriles was found to be catalysed by a nitrilase. The characteristics of the purified enzyme revealed that this is a new nitrilase as it has a molecular mass of 45 kDa and acts as a monomer. The optimum pH and temperature for the activity of the purified enzyme were 7.5 and 35° C, respectively. Thiol-specific reagents caused inhibition whereas chelators did not significantly alter the activity of this enzyme. The amino acids produced were of L-form, except for alanine. In the case of leucine production from α-aminoisocapronitrile, the enantiomeric ratio of L-leucine to D-leucine was about 60.
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Bhalla, T.C., Miura, A., Wakamoto, A. et al. Asymmetric hydrolysis of α-aminonitriles to optically active amino acids by a nitrilase of Rhodococcus rhodochrous PA-34. Appl Microbiol Biotechnol 37, 184–190 (1992). https://doi.org/10.1007/BF00178168
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DOI: https://doi.org/10.1007/BF00178168