Abstract
Nicotinic acid, pyridoxine, and picloram were stable in a liquid MS culture medium (pH 5.5–5.6) during autoclaving and during cell-free incubation in the dark at 5°C or 25°C for up to 6 weeks. Thiamine loss under the same conditions was 16% at 5°C and 18% at 25°C. Five percent of the sucrose in the liquid medium was hydrolyzed during autoclaving. During cell-free incubation in the light (100 μE m−2 s−1) at 25°C, pyridoxine was not detected after 6 days, while 78% of the picloram and 56% of the thiamine were degraded after 6 weeks. All of the niacin and pyridoxine, 13% of the picloram and 42% of the thiamine in a liquid MS culture medium were utilized in 4 days by potato (cv. Lemhi Russet) tuber suspension cultures growing in the dark at 25°C.
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Abbreviations
- BS:
-
Gamborg et al. medium (1968)
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indoleacetic acid
- MS:
-
Murashige & Skoog (1962)
- NAA:
-
naphthaleneacetic acid
- PAA:
-
phenylacetic acid
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Hagen, S.R., Muneta, P., Augustin, J. et al. Stability and utilization of picloram, vitamins, and sucrose in a tissue culture medium. Plant Cell Tiss Organ Cult 25, 45–48 (1991). https://doi.org/10.1007/BF00033911
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DOI: https://doi.org/10.1007/BF00033911