Abstract
Hellenic wheat germplasm developed at the Cereal Institute of Thessaloniki, during the last fifty years, is depicted in the present study, using agronomical, biochemical and molecular criteria. The bread wheat germplasm is characterized by high yield potential, adaptation to the Mediterranean conditions, and advanced qualitative traits. The biochemical analysis revealed that three cultivars (“Acheron”, “Elissavet” and “Orpheus”) carry the 1BL.1RS wheat-rye translocation, which was further confirmed by molecular analysis. The cultivars “Acheron” and “Elissavet”, despite the presence of the translocation, have good bread making quality due to the presence of favorable genes controlling quality. Most of the Hellenic durum germplasm have high end product quality. The biochemical evaluation at the Gli-B1 locus revealed the presence of alleles related with the γ-45 component, indicative of high technological traits. On the other side, it was found that two older durum wheat cultivars and a population tested, had the allele in* at the same locus, which is related with the γ-42 component, indicative of inferior pasta quality. Most of the Hellenic germplasm, either durum or bread wheat, carries very good agronomical and qualitative traits and could be used to broaden wheat biodiversity in the S–E Mediterranean region.
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INTRODUCTION
The rise of wheat breeding in Hellas begun in 1923, when Ioannis Papadakis (1903–1996), one of the most brilliant European breeders, established the first experiments to evaluate various domestic and foreign wheat cultivars. In 1927 the “Plant Breeding Institute” was founded in Thessaloniki aiming to promote research on plant breeding and more precisely in wheat [1]. At the same period, a network of substations was developed in the main wheat-producing regions of the country (Larissa, Halkidiki, Serres, Ptolemaida, Crete etc.), to broaden the adaptation of the resulting germplasm. The main vision of Prof. Papadakis was the self-fulfilment of the country’s needs in wheat. To accomplish this target, Papadakis introduced foreign cultivars from countries with similar soil and climatic conditions and at the same time bred new wheat cultivars after crossing domestic to foreign germplasm [2]. Despite the difficulties of the interwar period, Papadakis’ breeding program was successful and new high yielding cultivars, were released. In the same period, Papadakis developed the ecological maps of Hellas, promoting the cultivation of certain cultivars depending on the soil and climate of each region. In order to succeed in his vision, Papadakis developed unique approaches (pocket method/experiments in pots) for breeding new wheat germplasm [2–5].
The main goal of the described efforts was the rapid increase in wheat production and the country wheat self-sufficiency which was achieved in Hellas in 1957 [6]. It must be mentioned that this wheat self-sufficiency was recorded despite the reduction of the wheat harvested areas. A consequence of the previous goal was the increase of the wheat harvested area cultivated with Hellenic varieties from 21% in 1931 to 31% in 1939 and 60% in 1952 [7]. The most important release of Papadakis’s effort was cultivar G-38290, more widely known as the “Number”, which was produced in 1934. Although G-38290 entered the seed production in 1942, its multiplication was very slow until 1950 mainly due to the prevailing political conflicts in Hellas. However, this cultivar became popular to the Hellena farmers and it was cultivated for nearly 20 years covering 70% of wheat harvested area [8].
Modern Hellenic wheat cultivars are a result of an intensive breeding program which started late in the 1950s. Based on Papadakis’s excellent approach, modern breeders developed high yielding cultivars, which combined unique traits of Hellenic (local) and Mexican (CIMMYT-International Maize and Wheat Improvement Centre) germplasm [9]. Most of these cultivars are spring wheats sown in October to exploit the prevailing moderate winter conditions of southern Balkan Peninsula.
In the following sections a detailed description of the Hellenic bread and durum wheat germplasm will be attempted using morphological, agronomical, technological biochemical and molecular criteria. The Hellenic wheat germplasm is widely adapted, carries a considerable number of valuable genes conferring resistance to abiotic and biotic stress conditions and could be used by breeders in crossing programs for the production of new cultivars extending wheat biodiversity in the South-West region of Europe.
BREAD WHEAT
Yield Potential
Modern Hellenic bread wheat (Triticum aestivum L.) cultivars (Table 1) are adequately resistant to low temperatures (winter and spring) and to drought conditions [10–13]. It must be noted that drought conditions during the spring season (the grain filling period) is the main factor affecting negatively wheat production in the Mediterranean zone. Most of the Hellenic cultivars, which are presented in Table 1, were originally registered in the National Catalogue of cultivated plant varieties and are characterized by moderate to very good tillering efficiency and are quite resistant to lodging [10–13]. The involvement of CIMMYT’s germplasm in Hellenic breeding program resulted in selection of cultivars of broader adaptation. For this reason, the Hellenic cultivars could also be used in other countries with cropping conditions similar to those prevailing in Hellas [14]. This is getting more important if considering the excellent bread making quality of some of the aforementioned cultivars (e.g. “Apollonia”, “Acheron”, “Orpheus”, “Elissavet”) (Table 2).
Technological Traits
The main technological traits of the modern bread wheat cultivars illustrate the progress that has been achieved during the lasts two decades in Hellenic breeding programs (Table 2). Based on old local varieties, Hellene breeders succeeded in producing new germplasm combining the Hellenic gene pool with CIMMYT’s elite wheat germplasm. According to the Cereal Chemistry and Technology Department (CCTD) of the Cereal Institute of Thessaloniki (today Plant Breeding and Genetic Resources Institute), bread wheat varieties are classified according to their bread making quality in three categories: High (A), Intermediate (B) and Low (C). This classification was based on the following formula, developed at the CCTD of Cereal Institute [15]:
where P is the protein content, S is sedimentation value and V is the valorimetric number. When CIv < 300 the quality is low, when 300 < CIv < 500 the quality is intermediate and when CIv > 500 the quality is high.
It becomes evident from the data presented in Table 2, that most of the Hellenic cultivars could be attributed to the Intermediate or High category with respect to their bread making quality. The combination with the broad adaptation for the majority of these cultivars offers a good alternative for cultivation in other parts of South-eastern Europe with similar to the Hellenic weather conditions.
In an attempt to explain field performance and qualitative determination of the Hellenic germplasm, Xynias et al. [32] examined a quite large group of Hellenic bread wheat cultivars and selections with the use of biochemical markers (seed storage proteins). The majority of this germplasm (25 cultivars, Table 3) was produced at the Cereal Institute of Thessaloniki (Hellas), two cultivars (“Chios” and “Mykonos”) were produced at the Laboratory of Genetics and Plant Breeding of the Aristotle University of Thessaloniki [33], and one was originated from Russia [“Kavkaz/Cgn”, (“KVZ/Cgn”)]. Three of the Hellenic cultivars (“Acheron”, “Elissavet” and “Orpheus”) were found to carry the Gli-B1l allele which marks for the wheat-rye 1BL.1RS translocation. The 1BL.1RS translocation is of special interest because cultivars possessing it are resistant to either biotic or abiotic stress conditions and more precisely to drought and low temperature [34, 35], most probably due to the better development of the root system [36]. However, it has a negative consequence because this translocation is responsible for quality deterioration [37]. Rabinovich [38] reported that the 1BL.1RS translocation has been transferred to many current commercial varieties from the Russian ones (“Aurora”, “KVZ/Cgn” and “Skorospelka 35”). Among the three aforementioned Russian varieties the cultivar “KVZ/Cgn” has been repeatedly involved in the breeding program of the Cereal Institute in Thessaloniki because of its efficient combining ability. Thus, it is quite reasonable that the 1BL.1RS translocation was transferred to the cultivars “Acheron”, “Elissavet” and “Orpheus” from “KVZ/Cgn”. “Acheron” and two other cultivars (“Yecora E” and “Melia”) have especially good combinations with high molecular weight (HMW) glutenin subunits, carrying alleles Glu-A1a, Glu-B1i and Glu-D1d which according to Payne et al. [39] result in excellent bread-making quality.
One more interesting point in the study of Xynias et al. [32] is the presence of the 1BL.1RS wheat-rye translocation that was detected in the cultivar “Chios”. More precisely, the electrophoretic analysis of this cultivar revealed that it was consisting of a mixture of different genotypes (with respect to the alleles at the Gli-B1 and Glu-B1 locus, Table 3) one of which possessed the Gli-B1l allele which marks the wheat-rye 1BL.1RS translocation. This was rather odd and for this reason Peros et al. [40] examined seven of the aforementioned Hellenic cultivars (“Orpheus”, “Acheron”, “KVZ/Cgn”, “Elissavet”, “Vergina”, “Acheloos” and “Chios”) using specific primers against Sec l and Gli-B1 genes which are present on 1RS and 1BS chromosome arms of rye and wheat respectively. From the results of the above study it was verified that the cultivars “Orpheus”, “Acheron”, “KVZ/Cgn”, “Elissavet” do carry the 1BL.1RS translocation. However, the presence of the translocation was not detected in cultivar “Chios”. This could be attributed to the fact that different seed samples were analysed. The other two cultivars (“Vergina” and “Acheloos”) do not carry the translocation, confirming the previous report of Xynias et al. [32].
Even though the presence of the 1BL.1RS translocation is probably influenced by the genetic background of wheat [41], this translocation might be very useful. All four cultivars that were found carrying the 1BL.1RS translocation could be used in crosses to produce germplasm resistant to stresses (especially drought which is the most severe problem in the southern part of Europe). If the necessary precautions are taken during transferring this translocation to current cultivars (i.e. one of the parents must carry alleles Glu-A1a, Glu-B1i and Glu-D1d), then it would appear possible to develop high yielding varieties, resistant to lodging and biotic factors and exhibiting good qualitative traits [42]. The cultivars “Acheron” and “Elissavet” are good examples of such an approach. Successful results have been reported also in Serbia by Misic et al. [34].
The dendrogram constructed according to the presence of alleles encoding seed storage proteins revealed that the Hellenic bread wheat germplasm can be divided into three major groups (Fig. 1). The first one (I) includes only the related cultivars “Dodoni” and “Eurydice”, and also three lines developed after intervarietal selection into cultivar “Nestos” (1). The second group (II) divided into two subgroups, one (2) includes the cultivars “Mykonos”, “Elissavet” and “Lydia” and the other (3) which is more complicated, containing the related cultivars “Penios”, “S. Cerros E”, “Gorgona”, “Louros” as they have some common origin and also a minor group with “Strymonas”, “Acheloos” and “Oropos”. Despite the presence of the 1BL.1RS wheat-rye chromosome translocation the cultivar “Elissavet” is closer to “Lydia” and “Myconos” if someone compares it with the cultivar “KVZ/Cgn”, one of the donors of the translocation. According to the third major group (III), the cultivar “Acheron” (which is the second Hellenic variety possessing the translocation) was found very close to “Yecora E” (a cultivar with excellent bread making quality) in subgroup (4) and this could be an evidence of its good bread making quality. The cultivar “Orpheus”, the third Hellenic variety carrying the translocation, which is produced after intervarietal selection in the cultivar “Nestos”, is also close to “KVZ/Cgn” as it was confirmed in subgroup 5.
In a more recent study, Karelov et al. [43] investigated the genetic relationship between eight Hellenic bread wheat cultivars (“Acheron”, “Chios”, “Elissavet”, “Louros”, “Lydia”, “Strymonas”, “Xenia” and “Yecora E”) and the Russian cultivar “KVZ/Cgn” with molecular markers. It was revealed that the cultivar “Elissavet” carries genes conferring resistance to tan spot (insensitivity to toxins A and B), rusts, powdery mildew, and barley yellow dwarf virus (Lr34/Yr18/Pm38/Sr57/Bdv1) in combination with the genes on the wheat-rye 1BL.1RS translocation. Also, the cultivar “Strymonas” has three genes for resistance to necrotrophic diseases. The cultivar “Yecora E” carries the genes conferring resistance to tan spot and rusts (Lr34/Yr18/Pm38/Sr57/Bdv1) but lacks the translocation. It should be noted that the important gene Lr34/Yr18/Pm38/Sr57/Bdv1 is rare among European wheat cultivars [44]. The third cultivar “Acheron”, which carries the 1BL.1RS wheat-rye chromosome translocation, also has genes for resistance to tan spot (due to insensitivity to toxin B) and Fusarium head blight but lacks the resistance allele of the Lr34 gene. Furthermore, it is concluded from all the results that cultivar “Elissavet” constitutes a remarkable combination of favourable genes and is proposed to be used more extensively as a parental line in Mediterranean breeding programs for the development of novel wheat germplasm.
DURUM WHEAT
Durum wheat (Triticum durum Desf.) is an important crop used primarily for making pasta products, and in some cases for making bread [45]. As a crop, it is well adapted in the warm and dry areas of the Mediterranean region. The vast diversity of the Hellenic countryside, with thousands of bigger and smaller islands resulted in the development and preservation of many isolated local varieties and populations of durum wheat. As in the case of bread wheat, this germplasm was effectively used by breeders, who produced some excellent cultivars in terms of quality. Hellenic durum cultivars belong to the spring-type wheats, and for this a special effort was given by local breeders to produce durum germplasm which at least could tolerate the low temperatures prevailing in winter of the region. Thus, the majority of modern cultivars are at least moderate resistant to the low temperature of winter and spring (Table 4). They are also characterized by moderate tillering efficiency and the majority of them are adequately resistant to lodging [10–12]. The involvement of CIMMYT’s germplasm in Hellenic breeding programs resulted in the production of broadly adapted cultivars, which combine the traits of the local varieties and those of CIMMYT’s germplasm.
The main technological traits of the modern durum wheat cultivars illustrate the progress that has been achieved during the last decades in Hellenic wheat breeding programs (Table 5). As it was mentioned before, based on old local varieties, Hellene breeders succeeded in producing new germplasm, combining the Hellenic gene pool with CIMMYT’s elite wheat germplasm. According to the CCTD of the Cereal Institute of Thessaloniki, durum wheat varieties are classified for pasta making quality in three categories: High (A), Intermediate (B) and Low (C). For this classification the following formula developed by the CCTD of Cereal Institute [31] is used:
where P is the protein content and G is the percentage of vitreous grains. When CId < 500 the quality is low, when 500 < CId < 700 the quality is intermediate and when CId > 700 the quality is high.
It becomes evident from Table 5 that most of the modern Hellenic cultivars produce very qualitative products. This is evident even though the allele Gli-B1in*, with the component γ-42, similar to the allele a according to Kudryavtsev [46] was detected in two of the transferring cultivars (“Lemnos” and “Syros”) [47]. Similar results for “Lemnos” were reported by Yupsanis [48]. It was demonstrated that γ‑gladins encoded by genes at the Gli-B1 locus could serve as genetic markers for gluten quality [49]. This observation is based mostly to their linkage with low molecular weight gliadins (LMW-GS) encoded by genes at the Glu-B3 locus [50]. Later studies have shown that virtually always the presence of the gladin γ-42 component was associated with the LMW GS alleles Glu-B3b and i and is related with poor pasta quality [38, 47, 51].
Xynias et al. [47] studied 24 Hellenic durum cultivars and local populations using biochemical genetics methods based on gliadin and high-molecular-weight glutenin subunit loci (Table 6). In that study it was demonstrated that three of the local populations registered as durum were actually bread wheat. Furthermore, it was revealed that most modern Hellenic durum cultivars, including the newly-released ones, carry the allele combination Gli-A1r, Gli-B1h, Glu-A1c, Glu-B1b, Gli-A2-1. This combination is most likely due to the association of these alleles with grain quality [45]. A similar predominant association is observed also in the Hellenic local populations at three marker loci: Gli-A1r, Gli-B1h, Glu-A1c. Besides the association with grain quality, the presence of similar predominant alleles at the storage protein loci both in cultivars and local populations may indicate their adaptive value: the presence of these marker alleles may be associated with tolerance to stress factors during vegetation. At least partial adaptive value of protein polymorphism was demonstrated in emmer wheat by Nevo et al. [52]. Correlation of the genetic variation and the allele frequencies at HMW glutenin subunit loci in wild emmer wheat and their associations to the climatic and natural factors were also revealed [53, 54].
Most of current durum wheat Hellenic cultivars were found to be more or less identical at the loci studied (Fig. 2). This elucidates the narrowing of the genetic background of the crop (especially at certain loci), suggesting that more variability should be incorporated in modern breeding programs. A part of this variability could be mined in the local populations, which forms, and the second major group of the present study. The local “population of Thessalia” (Central Hellas) was more related to the respective “population of Heraklion” (Crete island), probably indicating some common dietary practices of local populations. The old cultivar “Lemnos” was found to be less related to other Hellenic cultivars and populations. This cultivar, as was previously referred, carries the gene locus Gli-B1 component γ 42, which is associated with inferior quality [49], and this was verified by all relevant quality tests (Cereal Institute of Thessaloniki, unpublished data). The cultivar “Lemnos” is a tall cultivar, susceptible to lodging. However, due to its cold resistance, especially in spring, and its early heading emergence [10] it is still quite a popular germplasm used in specific breeding projects.
The allelic diversity of the storage protein loci of the homoeologous group 1 chromosomes (Glu-A1, Glu-B1, Gli-A1, Gli-B1), which are more associated with quality compared to the Gli-2 locus [49], was broader in the Hellenic local populations of durum wheat than that recorded in modern Hellenic varieties. This indicates their potential utility in breeding programs for widening the gene pool of commercial cultivars.
FUTURE PROSPECTS AND CONCLUSIONS
The application of anther-culture technique for breeding new bread wheat germplasm is expected to contribute in producing new, high-quality cultivars [55, 56]. The results of this application until now are promising because two doubled-haploid lines (DHL 16 and DHL 17, originating from the cross “Penios” × “KVZ/Cgn”), were found to carry the gliadin allele Gli-B1l (Gli-B1-3) which as mentioned before marks the wheat-rye 1BL.1RS translocation [57]. These lines showed the highest indices of resistance to powdery mildew, leaf rust and Septoria tritici Blotch during all the vegetation stages [58]. They carried the Glu-A1b and Glu-D1d alleles [57] associated with high quality [59], supporting the view that high-quality cultivars could be produced, despite the presence of the 1BL.1RS wheat-rye translocation.
The majority of the new Hellenic wheat germplasm, either durum or bread, carry very good agronomical and qualitative traits and thus are adapted to SE Mediterranean conditions. Unique gene combinations present in old and local durum varieties are an interesting source for breeding new cultivars. The presence of resistant genes, and of course the presence of the wheat-rye 1BL.1RS translocation, could be very helpful in reducing chemical application during cropping under Mediterranean environment. Thus, wheat cultivation in Hellas, under the quite optimal for the crop growing conditions, guaranties the production of safe and qualitative end products. The involvement of old local germplasm at higher rates in wheat breeding programs, along with the application of cytogenetic, biochemical and molecular approaches, promises that new and more sophisticated varieties could be produced in the future.
REFERENCES
Papadakis, I.S., The Plant Breeding Institute 1923–1933, Sci. Bull. Inst. Plant Breed., 1933, vol. 15, pp. 1–36.
Papadakis, I.S., Statistical method for field experiments, Sci. Bull. Inst. Plant Breed., 1937, vol. 23, pp. 1–30.
Papadakis, I.S., Soil reaction and varietal adaptation, Sci. Bull. Inst. Plant Breed., 1935, vol. 18, pp. 1–16.
Papadakis, I.S., Varieties experiments in pots, Sci. Bull. Inst. Plant Breed., 1935, vol. 20, pp. 1–15.
Papadakis, I.S., The pocket method of varieties experiments, Sci. Bull. Inst. Plant Breed., 1935, vol. 21, pp. 1–7.
Kokolios, V., The cultivated wheat varieties in Hellas, Minist. Agric. Bull., 1959, vol. 3, pp. 1–78.
Talellis, D.E., The Increase in Agricultural Production and the Plant Breeding Institute, Thessaloniki: Ministry of Agriculture, Directorate of Agricultural Research. 1952.
Papadakis, I.S., The Greek agricultural and economic miracle (1922–1983). Lessons for foreigners and for us, Proc. Acad. Athens, 1983, vol. 58, 562–580 (speech by Academician I. Papadakis).
Skorda E.A. The Breeding of bread wheat, in Suggestions on Cereals Meeting, February 19–20, 1979, Thessaloniki, Greece, Thessaloniki: Ministry of Agriculture—Cereal Institute of Thessaloniki, 1979, pp. 8–17.
Anonymous, List of Cereal and Legume Grains of the State Seed Production, Athens: Ministry of Agriculture. 1978.
Anonymous, Cereal Varieties, Thessaloniki: Hellenic Ministry of Agriculture. 1985.
Anonymous, National Agricultural Research Foundation in Cereal Seed Production, Thessaloniki: NAGREF—Cereal Institute, 2011.
Korpetis, E., Hellenic Agricultural Organization—DEMETER in Cereal Seed Production, Thessaloniki: HAO-DEMETER, 2016.
USDA, Crop Production in Greece and Italy, United States: Department of Agriculture, Foreign Agricultural Service, Commodity Intelligence Report, 2017. https://ipad.fas.usda.gov/highlights/2017/08/greeceitaly/ index.htm. Accessed November 11, 2017.
Kokolios, V. and Valtadoros, A., Factors, forms and criteria of wheat quality. Applications on breeding for quality research in Hellas, New Agricult. Rev., 1965, vol. 5, pp. 4–16.
Kokolios, V., Valtadoros, A., Dimopoulos, J., and Pattakou, B., Qualité des blés Grecs de la recolte 1963, Sci. Bull., 1965, vol. 4, p. 11.
Kokolios, V., Valtadoros, A., Dimopoulos, J., and Pattakou, B., Qualité des blés Grecs de la recolte 1964, Sci. Bull., 1966, vol. 11, p. 16.
Boudonas, G., Valtadoros, A., Dimopoulos, J., and Pattakou, B., Qualité des blés Grecs de la recolte 1965, Sci. Bull., 1966, vol. 14, p. 35.
Boudonas, G., Valtadoros, A., Dimopoulos, J., and Pattakou, B., Qualité des blés Grecs de la recolte 1967, Sci. Bull., 1968, vol. 24, pp. 36.
Boudonas, G., Valtadoros, A., Dimopoulos, J., and Pattakou, B., Qualité des blés Grecs de la recolte 1970, Sci. Bull., 1971, vol. 45, p. 36.
Boudonas, G., Valtadoros, A., and Pattakou, B., Qualité des blés Grecs de la recolte, Sci. Bull., 1973, vol. 50, p. 32.
Boudonas, G., Valtadoros, A., Pattakou, B., and Papastephanou, S., Qualité des blés Grecs de la recolte 1973, Sci. Bull., 1974, vol. 53, p. 32.
Boudonas, G., Valtadoros, A., Pattakou, B., and Papastephanou, S., Qualité des blés Grecs de la recolte 1973, Sci. Bull., 1974b, vol. 54, p. 32.
Boudonas, G., Pattakou, B., Papastephanou, S., Gioupsanis, T., Qualité des blés Grecs de la recolte 1974, Sci. Bull., 1976a, vol. 56, p. 36.
Boudonas, G., Pattakou, V., Papastephanou, S., and Gioupsanis, T., Qualité des blés Grecs de la recolte 1975, Sci. Bull., 1976b, vol. 58, p. 46.
Pattakou, V., Papastephanou, S., Gioupsanis, T., and Triantafillakos, N., Qualité des blés Grecs de la recolte 1978, Sci. Bull., 1979, vol. 66, p. 30.
Pattakou, V., Papastephanou, S., Triantafillakos, N., and Hadjisavva, S., Wheat quality, Crop 1985, Sci. Bull., 1986, vol. 85, p. 30.
Irakli, M. and Papageorgiou, M., Quality of bread and durum wheat crop 2000, NAGREF—Cereal Institute of Thessaloniki, Sci. Bull.—New Ser., 2001, vol. 1, p. 30.
Papageorgiou, M. and Irakli, M., Quality of bread and durum wheat crops 2001 and 2002. NAGREF—Cereal Institute of Thessaloniki, Sci. Bull.—New Ser., 2004, vol. 2, p. 44 (in Hellenic with English abstract).
Papageorgiou, M. and Irakli, M., Quality of bread and durum wheat crops 2003 and 2004, NAGREF—Cereal Institute of Thessaloniki, Sci. Bull.—New Ser., 2005, vol. 3, p. 20.
Papageorgiou, M. and Irakli, M., Quality of bread and durum wheat crops 2005 and 2006, Sci. Bull.—New Ser., 2007, vol. 4, p. 24.
Xynias, I.N., Kozub, N.O., and Sozinov, I.A., Seed storage protein composition of Hellenic bread wheat cultivars, Plant Breed., 2006, vol. 125, pp. 408–410. https://doi.org/10.1111/j.1439-0523.2006.01242.x
Gouli-Vavdinoudi, E.K., Development of bread wheat varieties by selection in the absence of competition (T. aestivum L. Thell), Ph. D. Thesis, Thessaloniki: Scientific Yearbook of the Department of Agriculture Annex 8 of the 25th Volume, 1984.
Misic, T., Petrovic, S., and Mladenov, N., Characteristic of major Novi Sad winter wheat cultivars carrying wheat–rye translocation, in Proc. Int. Symp. Breed. Small Grains, November 24–27, 1998, Kragujevac, Yugoslavia, 1998, pp. 85–95.
Howell, T., Hale, I., Jankuloski, L., Bonafede, M., Gilbert, M., and Dubcovsky, J., Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status, Theor. Appl. Genet., 2014, vol. 127, pp. 2695–2709. https://doi.org/10.1007/s00122-014-2408-6
Ehdale, B., Whitkus, R.W., and Waines, J.G., Root biomass, water-use efficiency, and performance of wheat rye translocations of chromosomes 1 and 2 in spring bread wheat “Pavon”, Crop Sci., 2003, vol. 43, pp. 710–717. https://doi.org/10.2135/cropsci2003.7100
Carver, B.F. and Rayborn, A.L., Comparison of related wheat stocks possessing 1B or T1B1-Center-Dot-1Rs chromosome-grain and flour quality, Crop Sci., 1994, vol. 35, pp. 1316–1321. https://doi.org/10.2135/cropsci1994.0011183X003400060017x
Rabinovich, S.V., Importance of wheat–rye translocations for breeding modern cultivars of Triticum aestivum L., Euphytica, 1998, vol. 100, pp. 323–340. https://doi.org/10.1023/A:1018361819215
Payne, P.I., Holt, L.M., Jackson, E.A., and Law, C.N., Wheat storage proteins: their genetics and their potential for manipulation by plant breeding, Philos. Trans. R. Soc. London, 1984, vol. 304, pp. 359–371.
Peros, Ch., Dalezios, G., Liakakos, E., Delis, K., and Xynias, I.N., Verification of the presence of the 1BL.1RS translocation in Hellenic bread wheat cultivars using the PCR method, in Book of Abstracts of the 14th Congress of the Hellenic Society of Genetics and Plant Breeding, Thessaloniki, October 10–12, 2012, Thessaloniki, 2012, p. 70.
Mckendy, A.L., Taque, D.N., Finney, P.L., and Miskin, K.E., Effect of 1BL.1RS on milling and baking quality of soft red winter wheat, Crop Sci., 1996, vol. 36, pp. 848–851. https://doi.org/10.2135/cropsci1996.0011183X003600040004x
Dimitrijevic, M., Petrovic, S., and Gustafson, J.P., The presence of wheat/rye translocation 1BL.1RS in wheat, in Proc. 2nd Balkan Symp. on Field Crops, June 16–20, 1998, Novi Sad, Yugoslavia, Novi Sad, 1998, pp. 211–213.
Karelov, A.V., Kozub, N.I., Sozinov, I.A., Sozinova, O., and Xynias, I.N., Molecular detection of the resistance to biotic stress conditions in Hellenic bread wheat commercial cultivars, in Proc. HAICTA 2017 Conf., September 21–25, 2017, Chania, Crete, Hellas, 2017.
Kolmer, J.A., Singh, R.P., Garvin, D.F., Viccars, L., William, H.M., Huerta-Espino, J., Ogbonnaya, F.C., Raman, H., Orford, S., Bariana, H.S., and Lagudah, E.S., Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm, Crop Sci., 2008, vol. 48, pp. 1841–1852.https://doi.org/10.2135/cropsci2007.08.0474
Sissons, M., Role of durum wheat composition on the quality of pasta and bread, Food, 2008, vol. 2, pp. 75–90.
Kudryavtsev, A.M., Development of the system of genetic markers of durum wheat (T. durum Desf.) and its use in scientific research and practice, Extended Abstract of Doctoral (Biol.) Dissertation, Moscow, Russia, 2007.
Xynias, I.N., Kozub, N., and Sozinov, I., Analysis of Hellenic durum wheat (Triticum turgidum L. var. durum) germplasm using gliadin and high-molecular-weight glutenin subunit loci, Cer. Res. Comm., 2011, vol. 39, pp. 415–425. https://doi.org/10.1556/CRC.39.2011.3.11
Yupsanis, T., Identification and technological evaluation of wheat cultivars with electrophoresis of their gliadins, Agricult. Res., 1983, vol. 7, pp. 157–167.
Damideaux, R., Autran, J.C., Grignak, P., and Feillet, P., Mise en evidence de relations applicables en selection entre l’ electrophoregramme des gliadines et les properties viscoelastiques du gluten de Triticum durum Desf, Comptes Rendes de l’Academie des Sci., 1978, vol. 287, pp. 701–704.
Skerritt, J.H., Gluten proteins: genetics, structure and dough quality—a review, Ag. Biotech. News Inform., 1998, vol. 10, pp. 247N–270N.
Carrillo, J.M., Martinez, M.C., Moita Brites, C., Nieto-Taladriz, M.Y., and Vasquez, J.F., Relationship between endosperm proteins and quality in durum wheat (Triticum turgidum L. var. durum), in Durum Wheat Improvement in the Mediterranean Countries, Royo, C., Nachit, M.M., Di Fonzo, N., and Araus, J.L., Eds., New Challenge Seminaires Mediterraneennes, 2000, vol. 40, pp. 463–467.
Nevo, E., Korol, A.B., Beiles, A., and Fahima, T., Evolution of Wild Emmer and Wheat Improvement, Heidelberg: Springer-Verlag, 2002.
Nevo, E. and Payne, P.I., Wheat storage proteins: diversity of HMW glutenin subunits in wild emmer from Israel. 1. Geographical patterns and ecological predictability, Theor. Appl. Genet., 1987, vol. 74, pp. 827–836. https://doi.org/10.1007/BF00222968
Levy, A.A. and Feldman, M., Ecogeographical distribution of HMW glutenin alleles in populations of the wild tetraploid wheat T. turgidum var. dicoccoides, Theor. Appl. Genet., 1988, vol. 75, pp. 651–658. https://doi.org/10.1007/BF00289134
Zamani, J., Gouli-Vavdinoudi, E., and Roupakias, D.G., Anther culture of Greek cultivars and F1 crosses in bread wheat (Triticum aestivum), in Abstracts of 7th Conference of the Greek plant Breeding and Genetics Society, October 21–23, Heraklio, Crete, Hellas, Heraclio, 1998.
Xynias, I.N., Zamani, I.A., Gouli-Vavdinoudi, E., and Roypakias, D.G., Effect of cold pre-treatment and incubation temperature on bread wheat (Triticum aestivum L.) anther culture, Cer. Res. Commun., 2001, vol. 29, pp. 331–338.
Kozub, N., Xynias, I.N., Sozinov, I., Lisova, G., Zamani, I.A., Gouli-Vavdinoydi, E., and Roupakias, D.G., Screening of high-quality bread wheat dihaploid lines by the use of biochemical markers, Russ. J. Plant Physiol., 2006, vol. 53, pp. 396–400. https://doi.org/10.1134/S1021443706030162
Lisova, G.M., Kozub, N.O., Sozinov, I.O., and Xynias, I.N., Donors of leaf rust, powdery mildew, and septoriosis resistance among Greek bread wheat cultivars and dihaploid lines, Plant Protec. Quaran., 2005, vol. 51, pp. 22–29.
Payne, P.I. and Lawrence, G., Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat, Cer. Res. Commun., 1983, vol. 11, pp. 29–34.
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The present work is dedicated to the memory of Dr. Elpis Skodra for her contribution in wheat breeding in Hellas.
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Xynias, I.N., Mavromatis, A.G., Korpetis, E.G. et al. Description and Characterization of Hellenic Wheat Germplasm for Agronomical and Seed Quality Parameters Using Phenotypical, Biochemical and Molecular Approaches. Cytol. Genet. 53, 337–347 (2019). https://doi.org/10.3103/S0095452719040108
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DOI: https://doi.org/10.3103/S0095452719040108