Abstract
Exosomes are of increasing research interest because they are integral to cell–cell communication and are implicated in various disease states. Here, we investigated the utility of using an ion-depletion zone in a microfluidic device to concentrate exosomes from the culture media of four types of cell lines. Furthermore, we evaluated the extent of damage to the exosomes following concentration by an ion-depletion zone microchannel device compared with exosomes concentrated by a conventional ultra-centrifugation technique. Our results conclusively demonstrate that significantly less damage is incurred by exosomes following passage through and concentration by the ion-depleted zone microchannel device compared to concentration by ultra-centrifugation. Our findings will help extend the utility of exosomes to various applications.
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Acknowledgements
JSPS KAKENHI Grant Numbers 17H03246, a Grant-in-Aid for Young Scientists (B) (Nos. 15K20989 and 17K17715), and JKA RING!RING! Project for the financial support (FY2015, No. 27-183).
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Mogi, K., Hayashida, K. & Yamamoto, T. Damage-less Handling of Exosomes Using an Ion-depletion Zone in a Microchannel. ANAL. SCI. 34, 875–880 (2018). https://doi.org/10.2116/analsci.17P462
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DOI: https://doi.org/10.2116/analsci.17P462