Abstract
Hydrogen sulfide (H2 S) has been recently found to be a gaseous signaling molecule in plants. In this work, we studied the role of H2 S in alleviating salinity stress during wheat grain germination (Triticum aestivum L. Yangmai 158). Pretreatment with NaHS, a H2 S donor, during wheat grain imbibition, could significantly attenuate the inhibitory effect of salinity stress on wheat germination. NaHS-pretreated grain showed higher amylase and esterase activities than water control. NaHS pretreatment differentially stimulated the activities of catalase (CAT), guaiacol peroxidase (POD) and ascorbate peroxidase (APX), decreased the level of malondialdehyde (MDA) and reduced NaCl-induced changes in plasma membrane integrity in the radicle tips of seedlings compared with water control. We conclude that H2 S plays an important role in protecting wheat grain from oxidative damage induced by salinity stress.
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Álvarez, C., García, I., Moreno, I., Pérez-Pérez, M.E., Crespo, J.L., Romero, L.C., Gotor, C. 2012. Cysteine-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile in Arabidopsis. Plant Cell 24:4621–4634.
Bloem, E., Haneklaus, S., Schnug, E. 2005. Significance of sulfur compounds in the protection of plants against pests and diseases. J. Plant Nutr. 28:763–784.
Bloem, E., Riemenschneider, A., Volker, J., Papenbrock, J., Schmidt, A., Salac, I., Haneklaus, S., Schnug, E. 2004. Sulphur supply and infection with Pyrenopeziza brassicae influence L-cysteine desulphydrase activity in Brassica napus L. J. Exp. Bot. 55:2305–2312.
Christou, A., Manganaris, G.A., Papadopoulos, I., Fotopoulos, V. 2013. Hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways. J. Exp. Bot. 64:1953–1966.
Deising, H., Nicholson, R.L., Haug, M., Howard, R.J., Mendgen, K. 1992. Adhesion pad formation and the involvement of cutinase and esterases in the attachment of uredospores to the host cuticle. Plant Cell 4:1101–1111.
Gao, S.P., Hu, K.D., Hu, L.Y., Li, Y.H., Han, Y., Wang, H.L., Lv, K., Liu, Y.S., Zhang, H. 2013. Hydrogen sulfide delays postharvest senescence and plays an antioxidative role in fresh-cut kiwifruit. HortScience 48:1385–1392.
García-Limones, C., Hervás, A., Navas-Cortés, J.A., Jiménez-Díaz, R.M., Tena, M. 2002. Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea (Cicer arietinum L.) and Fusarium oxysporum f. sp. ciceris. Physiol. Mol. Plant Pathol. 61:325–337.
García-Mata, C., Lamattina, L. 2010. Hydrogen sulphide, a novel gasotransmitter involved in guard cell signalling. New Phytol. 188:977–984.
Hu, L.Y., Hu, S.L., Wu, J., Li, Y.H., Zheng, J.L., Wei, Z.J., Liu, J., Wang, H.L., Liu, Y.S., Zhang, H. 2012. Hydrogen sulfide prolongs postharvest shelf life of strawberry and plays an antioxidative role in fruits. J. Agric. Food Chem. 60:8684–8693.
Jin, Z., Shen, J., Qiao, Z., Yang, G., Wang, R., Pei, Y. 2011. Hydrogen sulfide improves drought resistance in Arabidopsis thaliana. Biochem. Biophys. Res. Commun. 414:481–486.
Jin, Z., Xue, S., Luo, Y., Tian, B., Fang, H., Li, H., Pei, Y. 2013. Hydrogen sulfide interacting with abscisic acid in stomatal regulation responses to drought stress in Arabidopsis. Plant Physiol. Biochem. 62:41–46.
Leshem, Y., Seri, L., Levine, A. 2007. Induction of phosphatidylinositol 3-kinase-mediated endocytosis by salt stress leads to intracellular production of reactive oxygen species and salt tolerance. Plant J. 51:185–197.
Lisjak, M., Srivastava, N., Teklic, T., Civale, L., Lewandowski, K., Wilson, I., Wood, M.E., Whiteman, M., Hancock, J.T. 2010. A novel hydrogen sulfide donor causes stomatal opening and reduces nitric oxide accumulation. Plant Physiol. Biochem. 48:931–935.
Lisjak, M., Teklic, T., Wilson, I.D., Whiteman, M., Hancock, J.T. 2013. Hydrogen sulfide: Environmental factor or signalling molecule? Plant Cell Environ. 36:1607–1616.
Mustafa, A.K., Gadalla, M.M., Sen, N., Kim, S., Mu, W., Gazi, S.K., Barrow, R.K., Yang, G., Wang, R., Snyder, S.H. 2009. H2 S signals through protein S-sulfhydration. Sci. Signal 2:ra72.
Rausch, T., Wachter, A. 2005. Sulfur metabolism: A versatile platform for launching defence operations. Trends Plant Sci. 10:503–509.
Sekmen, A.H., Türkan, I., Takio, S. 2007. Differential responses of antioxidative enzymes and lipid peroxidation to salt stress in salt-tolerant Plantago maritima and salt-sensitive Plantago media. Physiol. Plant. 131:399–411.
Wang, R. 2012. Physiological implications of hydrogen sulfide: A whiff exploration that blossomed. Physiol Rev. 92:791–896.
Wang, Y., Li, L., Cui, W., Xu, S., Shen, W., Wang, R. 2012. Hydrogen sulfide enhances alfalfa (Medicago sativa) tolerance against salinity during seed germination by nitric oxide pathway. Plant Soil 351:107–119.
Wang, Y.S., Yang, Z.M. 2005. Nitric oxide reduces aluminum toxicity by preventing oxidative stress in the roots of Cassia tora L. Plant Cell Physiol. 46:1915–1923.
Yamamoto, Y., Kobayashi, Y., Matsumoto, H. 2001. Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots. Plant Physiol. 125:199–208.
Yang, G., Wu, L., Jiang, B., Yang, W., Qi, J., Cao, K., Meng, Q., Mustafa, A.K., Mu, W., Zhang, S., Snyder, S.H., Wang, R. 2008. H2 S as a physiologic vasorelaxant: hypertension in mice with deletion of cystathionine gamma-lyase. Science 322:587–590.
Zhang, H., Shen, W.B., Zhang, W., Xu, L.L. 2005. A rapid response of α-amylase to nitric oxide but not gibberellin in wheat seeds during the early stage of germination. Planta 220:708–716.
Zhang, H., Hu, L.Y., Hu, K.D., He, Y.D., Wang, S.H., Luo, J.P. 2008. Hydrogen sulfide promotes wheat seed germination and alleviates oxidative damage against copper stress. J. Integr. Plant Biol. 50:1518–1529.
Zhang, H., Tang, J., Liu, X.P., Wang, Y., Yu, W., Peng, W.Y., Fang, F., Ma, D.F., Wei, Z.J., Hu, L.Y. 2009. Hydrogen sulfide promotes root organogenesis in Ipomoea batatas, Salix matsudana, and Glycine max, J. Integr. Plant Biol. 51:1086–1094.
Zhang, H., Tan, Z.Q., Hu, L.Y., Wang, S.H., Luo, J.P., Jones, R.L. 2010. Hydrogen sulfide alleviates aluminum toxicity in germinating wheat seedlings. J. Integr. Plant Biol. 52:556–567.
Zhang, H., Hu, S.L., Zhang, Z.J., Hu, L.Y., Jiang, C.X., Wei, Z.J., Liu, J., Wang, H.L., Jiang, S.T. 2011. Hydrogen sulfide acts as a regulator of flower senescence in plants. Postharvest Biol. Technol. 60:251–257.
Zhu, J.K. 2001. Plant salt tolerance. Trends Plant Sci. 6:66–71.
Zhu, J.K. 2002. Salt and drought stress signal transduction in plants. Annu. Rev. Plant Biol. 53:247–273.
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Communicated by G.V. Horváth
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Ye, S.C., Hu, L.Y., Hu, K.D. et al. Hydrogen Sulfide Stimulates Wheat Grain Germination and Counteracts the Effect of Oxidative Damage Caused by Salinity Stress. CEREAL RESEARCH COMMUNICATIONS 43, 213–224 (2015). https://doi.org/10.1556/CRC.2014.0037
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DOI: https://doi.org/10.1556/CRC.2014.0037