Abstract
The European Synchrotron Radiation Facility (ESRF) is the oldest and most powerful 3rd generation synchrotron in Europe, providing X-rays to more than 40 experimental stations welcoming several thousand researchers per year. A major success story has been the ESRF's facilities for macromolecular crystallography (MX). These are grouped around 3 straight sections: On ID23 canted undulators accommodate ID23-1, a mini-focus tuneable energy end station and ID23-2, the world's first micro-focus beamline dedicated to MX; ID29 houses a single, mini-focus, tuneable energy end station; ID30 will provide three end stations for MX due in operation from mid-2014 to early 2015. Here, one branch of a canted X-ray source feeds two fixed-energy end stations (MASSIF-1, MASSIF-3). The second feeds ID30B, a variable focus, tuneable energy beamline. MASSIF-1 is optimised for automatic high-throughput experiments requiring a relatively large beam size at the sample position, MASSIF-3 is a high-intensity, micro-focus facility designed to complement ID23-2. All end stations are highly automated, equipped with sample mounting robots and large area, fast-readout photon-counting detectors. Experiment control and tracking is achieved via a combination of the MXCuBE2 graphical user interface and the ISPyB database, the former allowing user-friendly control of all beamline components, the latter providing data tracking before, after and during experiments.
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References
J. Lescar et al., ESRF Newsletter 28, 12 (1997).
H. Belrhali et al., ESRF Newsletter 28, 15 (1997).
V. Biou et al., ESRF Newsletter 28, 21 (1997).
E. Pebay-Peyroula et al., Science 277, 1676 (1997).
K. Luger et al., Nature 389, 251 (1997).
H. Hope, Acta Crystallogr. B-Struct. Sci. 44, 22 (1988) DOI:10.1107/S0108768187008632.
W.A. Hendrickson, C.M. Ogata, Macromol. Crystallogr. A 276, 494 (1997) DOI:10.1016/S0076-6879(97)76074-9.
J.L. Smith, Curr. Opin. Struct. Biol. 1, 1002 (1991) DOI:10.1016/0959-440X(91)90098-E.
S. Wakatsuki et al., J. Synchrotron Radiat. 5, 215 (1998) DOI:10.1107/S0909049597018785.
D. de Sanctis et al., J. Synchrotron Radiat. 19, 455 (2012) DOI:10.1107/S0909049512009715.
D. Nurizzo et al., J. Synchrotron Radiat. 13, 227 (2006) DOI:10.1107/S0909049506004341.
D. Flot et al., J. Synchrotron Radiat. 17, 107 (2010) DOI:10.1107/S0909049509041168.
A. Perrakis et al., Acta Crystallogr. Sect. D-Biol. Crystallogr. 55, 1765 (1999) DOI:10.1107/S0907444999009348.
P. Theveneau et al., J. Phys.: Conf. Ser. 425, (2013) DOI:10.1088/1742-6596/425/1/012001.
P. Pernot et al., J. Synchrotron Radiat. 20, 660 (2013) DOI:10.1107/S0909049513010431.
A. Round et al., Acta Crystallogr. D-Biol. Crystallogr. 71, 67 (2015) DOI:10.1107/S1399004714026959.
A. Round et al., Acta Crystallogr. D-Biol. Crystallogr. 69, 2072 (2013) DOI:10.1107/S0907444913019276.
A. De Maria Antolinos et al., Acta Crystallogr. D-Biol. Crystallogr. 71, 76 (2015) DOI:10.1107/S1399004714019609.
D. von Stetten et al., Acta Crystallogr. D-Biol. Crystallogr. 71, 15 (2015) DOI:10.1107/S139900471401517X.
S. Malbet-Monaco et al., Acta Crystallogr. D-Biol. Crystallogr. 69, 1289 (2013) DOI:10.1107/S0907444913001108.
H.M. Berman et al., Nucl. Acids Res. 28, 235 (2000) DOI:10.1093/Nar/28.1.235.
E. Micossi, W.N. Hunter, G.A. Leonard, Acta Crystallogr. D-Biol. Crystallogr. 58, 21 (2002) DOI:10.1107/S0907444901016808.
F. Cipriani et al., Acta Crystallogr. D-Biol. Crystallogr. 62, 1251 (2006) DOI:10.1107/S0907444906030587.
T. Giraud et al., J. Appl. Crystallogr. 42, 125 (2009) DOI:10.1107/S0021889808040958.
G.A. Leonard et al., J. Appl. Crystallogr. 42, 333 (2009) DOI:10.1107/S0021889809001721.
J. Gabadinho et al., J. Synchrotron Radiat. 17, 700 (2010) DOI:10.1107/S0909049510020005.
D. de Sanctis, G. Leonard, in Notiziario Neutroni e Luce di Sincrotrone Vol. 19 (Consiglio Nazionale delle Ricerche, 2014) p. 24.
S. Delageniere et al., Bioinformatics 27, 3186 (2011) DOI:10.1093/bioinformatics/btr535.
M.W. Bowler et al., Acta Crystallogr. D-Biol. Crystallogr. 66, 855 (2010) DOI:10.1107/S0907444910019591.
G.P. Bourenkov, A.N. Popov, Acta Crystallogr. D-Biol. Crystallogr. 66, 409 (2010) DOI:10.1107/S0907444909054961.
J. Sanchez-Weatherby et al., Acta Crystallogr. D-Biol. Crystallogr. 65, 1237 (2009) DOI:10.1107/S0907444909037822.
S. Brockhauser et al., Acta Crystallogr. D-Biol. Crystallogr. 69, 1241 (2013) DOI:10.1107/S0907444913003880.
S. Brockhauser et al., Acta Crystallogr. D-Biol. Crystallogr. 68, 975 (2012) DOI:10.1107/S090744491201863x.
M.W. Bowler et al., Cryst. Growth Des. 15, 1043 (2015) DOI:10.1021/cg500890r.
S. Russi et al., J. Struct. Biol. 175, 236 (2011) DOI:10.1016/j.jsb.2011.03.002.
S. Monaco et al., J. Appl. Crystallogr. 46, 804 (2013) DOI:10.1107/S0021889813006195.
W. Kabsch, Acta Crystallogr. D-Biol. Crystallogr. 66, 125 (2010) DOI:10.1107/S0907444909047337.
T.G. Battye et al., Acta Crystallogr. D-Biol. Crystallogr. 67, 271 (2011) DOI:10.1107/S0907444910048675.
M.D. Winn et al., Acta Crystallogr. D-Biol. Crystallogr. 67, 235 (2011) DOI:10.1107/S0907444910045749.
P.D. Adams et al., Acta Crystallogr. D-Biol. Crystallogr. 66, 213 (2010) DOI:10.1107/S0907444909052925.
G.M. Sheldrick, Acta Crystallogr. A 64, 112 (2008) DOI:10.1107/S0108767307043930.
P. van der Linden et al., J. Appl. Crystallogr. 47, 584 (2014) DOI:10.1107/S1600576714000855.
A.A. McCarthy et al., J. Synchrotron Radiat. 16, 803 (2009) DOI:10.1107/S0909049509035377.
C. Gati et al., IUCrJ 1, 87 (2014) DOI:10.1107/S2052252513033939.
F. Stellato et al., IUCrJ 1, 204 (2014) DOI:10.1107/S2052252514010070.
C. Broennimann et al., J. Synchrotron Radiat. 13, 120 (2006) DOI:10.1107/S0909049505038665.
G. Hulsen et al., J. Appl. Crystallogr. 39, 550 (2006) DOI:10.1107/S0021889806016591.
R. Giordano et al., Acta Crystallogr. D-Biol. Crystallogr. 68, 649 (2012) DOI:10.1107/S0907444912006841.
J.L. Ferrer et al., Expert Opin. Drug Discov. 8, 835 (2013) DOI:10.1517/17460441.2013.793666.
M.G. Bowler, M.W. Bowler, Acta Crystallogr. F 70, 127 (2014) DOI:10.1107/S2053230X13032007.
G.A. Leonard et al., Acta Crystallogr. D-Biol. Crystallogr. 61, 388 (2005) DOI:10.1107/S0907444905000429.
R.B.G. Ravelli et al., J. Synchrotron Radiat. 12, 276 (2005) DOI:10.1107/S0909049505003286.
D. de Sanctis, M.H. Nanao, Acta Crystallogr. D-Biol. Crystallogr. 68, 1152 (2012) DOI:10.1107/S0907444912023475.
J. Harms et al., Cell 107, 679 (2001) DOI:10.1016/S0092-8674(01)00546-3.
B.T. Wimberly et al., Nature 407, 327 (2000).
S.G.F. Rasmussen et al., Nature 450, 383 (2007) DOI:10.1038/Nature06325.
T. Warne et al., Nature 454, 486 (2008) DOI:10.1038/Nature07101.
R. Baradaran et al., Nature 494, 443 (2013) DOI:10.1038/Nature11871.
A. Amunts et al., J. Biol. Chem. 285, 3478 (2010) DOI:10.1074/jbc.M109.072645.
J.E. Burke et al., Science 344, 1035 (2014) DOI:10.1126/science.1253397.
J. Fritsch et al., Nature 479, 249 (2011) DOI:10.1038/Nature10505.
M. Elias et al., Nature 491, 134 (2012) DOI:10.1038/Nature11517.
U.K. Eriksson et al., Science 340, 1346 (2013) DOI:10.1126/science.1234306.
A.P. Carter et al., Nature 407, 340 (2000).
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Contribution to the Focus Point on “Status of third-generation synchrotron crystallography beamlines: An overview” edited by Gaston Garcia.
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Mueller-Dieckmann, C., Bowler, M.W., Carpentier, P. et al. The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility. Eur. Phys. J. Plus 130, 70 (2015). https://doi.org/10.1140/epjp/i2015-15070-0
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DOI: https://doi.org/10.1140/epjp/i2015-15070-0