Abstract
Net blotch of barley (Hordeum vulgare L.), caused by Pyrenophora teres f. teres, and spot blotch, caused by Cochliobolus sativus, are the most widespread and harmful diseases in the geographic range of the crop. Barley breeding for resistance to these diseases should employ large genetic diversity. The 11_11067 SNP marker was revealed on chromosome 6H position 58 cM in the double haploid (DH) population A developed by crossing the Ethiopian accession k-23874, highly resistant to P. teres f. teres, to the susceptible Pirkka cultivar. It was reliably (p < 0.05) associated with resistance to three P. teres f. teres isolates. In population B (Zernogradsky 813 (MR to C. sativus) Ranniy 1 (MR to P. teres f. teres), 11 QTL controlling resistance to 12 P. teres f. teres isolates were found on all barley chromosomes and 14 QTL for resistance to 12 C. sativus isolates, on all chromosomes except for 4H. For both pathogens, the revealed QTL were shown to be isolate-specific. The majority of the loci detected were mapped in the same intervals between SNP markers where QTL controlling resistance to P. teres f. teres and C. sativus had been found by other scientists. Four novel QTL controlling resistance to P. teres f. teres were found on chromosomes 1H, 4H, and 5H. Five novel QTL associated with resistance to C. sativus were found on chromosomes 2H, 3H, 5H, and 6H in DH population B.
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Abu-Qamar M., Liu, Z.H., Faris, J.D., Chao, S., Edwards, M.C., Lai, Z., Franckowiak, J.D., and Friesen, T.L., A region of barley chromosome 6H harbors multiple major genes associated with net type net blotch resistance, Theor. Appl. Genet., 2008, vol. 117, no. 8, pp. 1261–1270.
Afanasenko, O.S., Laboratory method for assessing the resistance of barley cultivars to the causative agent of net blotch, S.-Kh. Biol., 1977, vol. 12, no. 2, pp. 297–299.
Afanasenko, O.S. and Levitin, M.M., The structure of populations of the causative agent of net blotch on the basis of virulence. I. Identification of races, Mikol. Fitopatol., 1979, vol. 13, no. 3, pp. 230–234.
Afanasenko, O.S. and Novozhilov, K.V., Problems of sustainable use of genetic resources of plant resistance to diseases, Ekol. Genet., 2009, vol. 7, no. 2, pp. 38–43.
Afanasenko, O.S., Zubkovich, A.A., and Makarova, I.G., Genetic control of resistance to Pyrenophora teres Drechs. strains in barley, Russ. J. Genet., 1999, vol. 35, no. 3, pp. 269–273.
Afanasenko, Î., Jalli, M., Pinnschmidt, H., Filatova, O., and Platz, G., Development of an international standard set of barley differential genotypes for Pyrenophora teres f. teres, Plant Pathol., 2009, vol. 58, pp. 665–676.
Afanasenko, O.S., Mironenko, N.V., Anisimova, A.V., Lashina, N.M., Radyukevich, T.N., Loskutov, I.G., and Novozhilov, K.V., Methodological support of barley breeding for resistance to leaf blotches, in Tekhnologii Sozdaniya i ispol’zovaniya sortov i gibridov s gruppovoi i kompleksnoi ustoichivost’yu k vrednym organizmam v zashchite rastenii (Technologies for Creation and Use of Cultivars and Hybrids with Group and Combined Resistance to Pests in Plant Protection), 2010, pp. 217–228.
Arru, L., Francia, E., and Pecchioni, N., Isolate-specific QTLs of resistance to leaf stripe (Pyrenophora graminea) in the Steptoe × Morex spring barley cross, Theor. Appl. Genet., 2003, vol. 106, pp. 668–675.
Cakir, M., Gupta, S., Platz, G.J., Ablett, G.A., Loughman, R., Embiri, L.C., Poulsen, D., Li, C.D., Lance, R.C.M., Galwey, N.W., Jones, M.G.K., and Appels, R., Mapping and validation of the genes for resistance to Pyrenophora teres f. teres in barley (Hordeum vulgare L.), Aust. J. Agric. Res., 2003, vol. 54, pp. 1369–1377.
Cakir, M., Gupta, S., Li, C., Hayden, M., Mather, D.E., Ablett, G.A., Platz, G.J., Broughton, S., Chalmers, K.J., Loughman, R., Jones, M.G.K., and Lance, R.C.M., Genetic mapping and QTL analysis of disease resistance traits in the barley population Baudin × AC Metcalfe, Cropand Pasture Science, 2011, vol. 62, no. 2, pp. 152–161.
Close, T.J., Bhat, P.R., Lonardi, S., Wu, Y., Rostoks, N., Ramsay, L., Druka, A., Stein, N., Svensson, J.T., Wanamaker, S., Bozdag, S., Roose, M.L., Moscou, M.J., Chao, S., Varshney, R.K., Szucs, P., Sato, K., Hayes, P.M., Matthews, D.E., Kleinhofs, A., Muehlbauer, G.J., DeYoung, J., Marshall, D.F., Madishetty, K., Fenton, R.D., Condamine, P., Graner, A., and Waugh, R., Development and implementation of high-throughput SNP genotyping in barley, BMC Genomics, 2009, vol. 10, p. 582.
D’yakov, Yu.T., Ozeretskovskaya, O.L., Dzhavakhiya, V.G., and Bagirova, S.F., Obshchaya i molekulyarnaya fitopatologiya (General and Molecular Phytopathology), Moscow: Obshch. Fitopatologov, 2001.
Emebiri, L.C., Platz, G., and Moody, D.B., Disease resistance genes in a doubled haploid population of tworowed barley segregating for malting quality attributes, Aust. J. Agric. Res., 2005, vol. 56, no. 1, pp. 49–56.
Fetch, T.G.J. and Steffenson, B.J., Rating scales for assessing infection responses of barley infected with Cochliobolus sativus, Plant Disease, 1999, vol. 83, pp. 213–217.
Le Gouis, J., Devaux, P., Werner, K., Hariri, D., Bahrman, N., Beghin, D., and Ordon, F., Rym15 from the Japanese cultivar Chikurin Ibaraki 1 is a new barley mild mosaic virus (BaMMV) resistance gene mapped on chromo- some 6H, Theor. Appl. Genet., 2004, vol. 108, no. 8, pp. 1521–1525.
Grewal, T.S., Rossnagel, B.G., Pozniakc, J., and Scoles, G.J., Mapping quantitative trait loci associated with barley net blotch resistance, Theor. Appl. Genet., 2008, vol. 116, pp. 529–539.
Grewal, T.S., Rossnagel, B.G., and Scoles, G.J., Validation of molecular markers associated with net blotch resistance and their utilization in barley breeding, Crop Sci., 2010, vol. 50, pp. 177–184.
Grewal, T.S., Rossnagel, B.G., and Scoles, G.J., Mapping quantitative trait loci associated with spot blotch and net blotch resistance in a doubled-haploid barley population, Mol. Breed, 2012, vol. 30, pp. 267–279.
Gul’tyaeva, E.I. and Volkova, G.V., Identification of genes for resistance to leaf rust in wheat varieties using molecular markers, Vestn. Zashchity Rast., 2009, no. 3, pp. 32–36.
Gul’tyaeva, E.I., Kanyuka, I.A., Alpat’eva, N.V., Baranova, O.A., Dmitriev, A.P., and Pavlyushin, V.A., Molecular approaches to the identification of genes for resistance to leaf rust in Russian wheat cultivars, Dokl. Ross. Akad. S.-Kh. Nauk, 2009, no. 5, pp. 23–26.
Gupta, S., Li, C.D., Loughman, R., Cakir, M., Platz, G., Westcott, S., Bradley, J., Broughton, S., and Lance, R., Quantitative trait loci and epistatic interactions in barley conferring resistance to net type net blotch (Pyrenophora teres f. teres) isolates, Plant Breed., 2010, vol. 4, pp. 268–362.
Gupta, S., Li, C., Loughman, R., Cakir, M., Westcott, S., and Lance, R., Identifying genetic complexity of 6H locus in barley conferring resistance to Pyrenophora teres f. teres, Plant Breed., 2011, vol. 130, pp. 423–429.
Gutierrez, L., Berberian, N., Capettini, F., et al., Genomewide association mapping identifies disease-resistance QTLs in barley germplasm from Latin America, in Advance in Barley Sciences. Proc. of 11th Intern. Barley Genet. Symp., 2013, pp. 209–215.
Hickey, L.H., Lawson, W., Platz, G.J., Dieters, M., Arief, V.N., German, S., Fletcher, S., Park, R.F., Singh, D., Pereyra, S., and Franckowiak, J., Mapping Rph20: a gene conferring adult plant resistance to Puccinia hordei in barley, Theor. Appl. Genet., 2011, vol. 123, pp. 1–25.
Ho, K.M., Tekauz, A., Choo, T.M., and Martin, R.A., Genetic studies on net blotch resistance in barley cross, Can. J. Plant. Sci., 1996, vol. 76, pp. 715–719.
Khan, T.N. and Tekauz, A., Occurence and pathogenicity of Drechslera teres isolates causing spot type symptoms on barley in Western Australia, Plant Dis., 1982, vol. 66, pp. 423–425.
Konig, J., Perovic, D., Kopahnke, D., and Ordon, F., Mapping seedling resistance to net form of net blotch (Pyrenophora teres f. teres) in barley using detached leaf assay, Plant Breed., 2014, vol. 133, pp. 356–365.
Kosambi, D.D., The estimation of map distances from recombination values, Ann. Eugen., 1944, vol. 12, pp. 172–175.
Krupin, P.Yu., Molecular cytogenetic characterization of the collection of intermediate wheat–wheatgrass hybrids, Extended Abstract of Cand. Sci. Dissertation, Moscow: Vseros. Nauch.-Issled. Inst. Sel’skokhoz. Biotekhnol. RASKhN, 2011.
Lander, E., Green, P., Abrahamson, J., Barlow, A., Daly, M., Lincoln, S., and Newburg, L., Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations, Genomics, 1987, no. 1, pp. 174–181.
Lapochkina, I.F., Gainullin, N.R., Dzhenin, S.V., Rudenko, M.I., Makarova, I.Yu., Iordanskaya, I.V., Kyzlasov, V.G., Kovalenko, E.D., and Zhemchuzhina, A.I., The use of molecular markers in the transfer of effective resistance genes from new donors in common wheat cultivars, in Mater. Konf. RASKhN-RFFI (Proc. Conf. RASKhN–RFFI), 2009, pp. 70–73.
Ma, Z.Q., Lapitan, N.L.V., and Steffenson, B., QTL mapping of net blotch resistance genes in a doubled-haploid population of six-rowed barley, Euphytica, 2004, vol. 137, pp. 291–296.
Manninen, O., Optimizing anther culture for barley breeding, Agr. foodsci. Finland, 1997, vol. 6, pp. 389–398.
Manninen O.M., Jalli M., Kalendar R., Schulman A., Afanasenko O., Robinson J. Mapping of major spot-type and net-type net blotch resistance genes in the Ethiopian barley (Hordeum vulgare) line CI 9819, Genome, 2006, vol. 49, pp. 1564–1571.
Marcel, T., Aghnoum, R., Durand, J., Varshney, R.K., and Niks, R.E., Dissection of the barley 2L1.0 region carrying the ‘Laevigatum’ quantitative resistance gene to leaf rust using near-isogenic lines (NIL) and subNIL, Mol. Plant Microbe Interact, 2007, vol. 20, no. 12, pp. 1604–1615.
Meldrum, S.I., Platz, G., and Ogle, H.J., Pathotypes of Cochliobolus sativus on barley in Australia, Aust. Plant Pathol., 2004, vol. 33, pp. 109–114.
Mikhailova, L.A. and Afanasenko, O.S., Application of excised leaves to study the resistance of cereals to diseases, Mikol. Fitopatol., 2005, no. 6, pp. 100–112.
Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 1962, vol. 15, no. 3, pp. 473–497.
Raman, H., Venkatanagappa, S., Rehman, A., Rehman, A., O’Bree, B., and Read, B., Graphical genotyping of barley using molecular markers linked with malting quality, disease resistance and aluminium tolerance, Barley Tech. Cereal Chem., 2003, pp. 246–249.
Rostoks, N., Ramsay, L., Mackenzie, K., Cardle, L., Bhat, P.R., Roose, M.L., Svensson, J.T., Stein, N., Varshney, R.K., Marshall, D.F., Graner, A., Clos, T.J., and Waugh, R., Recent history of artificial outcrossing facilitates whole-genome association mapping in elite inbred crop varieties, Proc. Natl. Acad. Sci. USA, 2006, vol. 103, pp. 18656–18661.
Roy, J.K., Smith, K.P., Muehlbauer, G.J., Chao, S., Close, T.J., and Steffenson, B.J., Associating mapping of spot blotch resistance in wild barley, Mol. Breed., 2010, vol. 26, pp. 243–256.
Saghai-Maroof, M.A., Soliman, K.M., Jorgenson, R.A., Jorgenson, R.A., and Allard, R.W., Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics, Proc. Natl. Acad. Sci. USA, 1984, vol. 81, pp. 8014–8018.
Serenius, M., Population structure of Pyrenophora teres the causal agent of net blitch of barley, Agrifood Res. Rep., 2006, vol. 78, p. 60.
Spaner, D.S.L., Falak, T.M., Choo, I., Legge, K.G., Briggs, W.G., Falk, D.E., Ullrich, S.E., Tinker, N.A., Steffenson, B.J., and Mather, D.E., Mapping of disease resistance loci in barley on the basis of visual assessment of naturally occurring symptoms, Crop Sci., 1998, vol. 38, pp. 843–850.
Steffenson, B.J. and Webster, R.K., Pathotype diversity of Pyrenophora teres f. teres on barley, Phytopathology, 1992, vol. 82, pp. 170–177.
Steffenson, B.J., Hayes, P.M., and Kleinhofs, A., Genetics of seedling and adult plant resistance to net blotch (Pyrenophora teres f. teres) and spot blotch (Cochliobolus sativus) in barley, Theor. Appl. Genet., 1996, vol. 92, pp. 552–558.
Tekauz, A., A numerical scale to classify reactions of barley to Pyrenophora teres, Can. J. Plant Pathol., 1985, vol. 7, pp. 181–183.
Tuohy, J.M., Jalli, M., Cooke, B.M., and Sullivan, E.O., Pathogenic variation in populations Dreschslera teres f. teres and D. teres f. maculata and differences in host cultivar responses, Eur. J. Plant Pathol., 2006, vol. 116, no. 3, pp. 177–185.
Tyryshkin, L.G., Gul’tyaeva, E.I., and Alpat’eva, N.V., Identification of effective leaf-rust resistance genes in wheat (Triticum aestivum) using STS markers, Russ. J. Genet., 2006, vol. 42, no. 6, pp. 662–666.
Valjavec-Gratian, M. and Steffenson, B.J., Pathotypes of Cochliobolus sativus on barley in North Dakota, Plant Dis., 1997, vol. 81, pp. 1275–1278.
Vasil’ev, A.V. and Bespalova, L.A., The first steps in the use of marker-mediated selection in breeding wheat cultivars in the Krasnodar Agricultural Research Institute named after P.P. Lukyanenko, in XI Molodezhnaya Konf. “Biotekhnologiya v rastenievodstve, zhivotnovodstve i veterinarii” (XI Youth Conf. “Biotechnology in Crop Production, Animal Husbandry, and Veterinary Medicine”), 2011, pp. 19–20.
Wagner, C., Schweizer, G., Kramer, M., Dhmer-Badani, A.G., Ordon, F., and Friedt, W., The complex quantitative barley–Rhynchosporium secalis interaction: newly identified QTL may represent already known resistance genes, Theor. Appl. Genet., 2008, vol. 118, pp. 113–122.
Zhou, H. and Steffenson, B.J., Association mapping of Septoria speckled leaf blotch resistance in us barley breeding germplasm, Phytopathology, 2013, vol. 103, pp. 600–609.
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Original Russian Text © O.S. Afanasenko, A.V. Koziakov, P.E. Hedlay, N.M. Lashina, A.V. Anisimova, O. Manninen, M. Jalli, E.K. Potokina, 2014, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2014, Vol. 18, No. 4/1, pp. 751–764.
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Afanasenko, O.S., Koziakov, A.V., Hedlay, P.E. et al. Mapping of the loci controlling the resistance to Pyrenophora teres f. teres and Cochliobolus sativus in two double haploid barley populations. Russ J Genet Appl Res 5, 242–253 (2015). https://doi.org/10.1134/S2079059715030028
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DOI: https://doi.org/10.1134/S2079059715030028