Abstract
The effects of transformation of downy birch (Betula pubescens Ehrh.) with the GS1 gene encoding the cytosolic form of glutamine synthetase on the rooting of plants in vitro was studied. The transgenic plants had an elevated content of glutamine as well as glutamic and aspartic acids and rooted more rapidly than the control plants. Rooting on a medium containing the glutamine synthetase inhibitor phosphinothricin prevented the accumulation of auxin in birch plants carrying the GS1 gene, indicating the involvement of this enzyme in raising the level of auxins in the transgenic plants. The correlation between the increase in the auxin levels in the transgenic plants carrying the glutamine synthetase gene and the increase in the rooting rate is shown for the first time.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Gewin, V., Nature, 2010, vol. 466, pp. 552–553.
Woodwart, A.W. and Bartel, B.K., Ann. Bot., 2005, vol. 95, no. 5, pp. 707–735.
Kudoyarova, G.R., Dodd, I.C., Veselov, D.S., Rothwell, S.A., and Veselov, S.Yu., J. Exp. Bot., 2015, vol. 66, pp. 2133–2144.
Shestibratov, A., Bulatova, I.V., and Novikov, P.S., Appl. Biochem. Microbiol., 2010, vol. 46, no. 8, pp. 763–768.
Man, H., Pollmann, S., Weiler, E.W., and Kirby, E.G., J. Exp. Bot., 2011, vol. 62, pp. 4423–4431.
Lebedev, V.G., Schestibratov, K.A., Shadrina, T.E., Bulatova, I.V., Abramochkin, D.G., and Miroshnikov, A.I., Russ. J. Genet., 2010, vol. 46, no. 11, pp. 1282–1289.
Veselov, S.Yu., Kudoyarova, G.R., Egutkin, N.L., Gyuli-Zade, V.Z., Mustafina, A.R., and Kof, E.M., Physiol. Plant., 1992, vol. 86, pp. 93–96.
Walch-Liu, P., Ivanov, I.I., Filleur, S., Gan, Y., Remans, T., and Forde, B.G., Ann. Bot., 2006, vol. 97, no. 5 P, pp. 875–881.
Kudoyarova, G.R., Melentiev, A.I., Martynenko, E.V., Arkhipova, T.N., Shendel, G.V., Kuz’mina, L.Yu., Dodd, I.C., and Veselov, S.Yu., Plant Physiol. Biochem., 2014, vol. 83, pp. 285–291.
Migge, A., Carrayol, E., Hirel, B., and Becker, T.W., Planta, 2000, vol. 210, pp. 252–260.
Zhu, C., Chen, S., Zhang, G., Wang, W., Tang, Y., Mei, B., and Song, R., Plant Biotech. Rep., vol. 9, pp. 187–195.
Urriola, J. and Rathore, K.S., Transgenic Res, 2015, vol. 24, pp. 397–407.
Walch-Liu, P. and Forde, B.G., Plant J., 2008, vol. 54, pp. 820–828.
Suárez, I., Bodega, G., and Fernández, B., Neurochem. Int., 2002, vol. 41, pp. 123–142.
Forde, B.G. and Walch-Liu, P., Plant Cell Environ., 2009, vol. 32, pp. 682–693.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © V.G. Lebedev, A.V. Korobova, G.V. Shendel, G.R. Kudoyarova, K.A. Shestibratov, 2018, published in Doklady Akademii Nauk, 2018, Vol. 480, No. 3, pp. 369–372.
Rights and permissions
About this article
Cite this article
Lebedev, V.G., Korobova, A.V., Shendel, G.V. et al. Effect of Glutamine Synthetase Gene Overexpression in Birch (Betula pubescens) Plants on Auxin Content and Rooting in vitro. Dokl Biochem Biophys 480, 143–145 (2018). https://doi.org/10.1134/S1607672918030043
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1607672918030043