Abstract
We studied the effect of a heterogeneous environment on the stereoselectivity of transformation of racemic phenylglycine nitrile. Immobilized biocatalysts were prepared by adhesion of Pseudomonas fluorescens C2 cells on carbon-containing supports and covalent crosslinking of nitrile hydratase and amidase of Rhodococcus rhodochrous 4–1 to activated chitosan as well as by the method of cross-linked aggregates. At a reaction duration of 20 h, the ratio of phenylglycine stereoisomers changes depending on the presence of support in medium. The highest optical purity of the product (enantiomeric excess of L-phenylglycine solution, 98%) is achieved when enzyme aggregates of nitrile hydratase and amidase cross-linked with 0.1% glutaraldehyde are used as a biocatalyst.
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Original Russian Text © Yu.G. Maksimova, A.N. Gorbunova, V.A. Demakov, 2017, published in Doklady Akademii Nauk, 2017, Vol. 474, No. 2, pp. 248–250.
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Maksimova, Y.G., Gorbunova, A.N. & Demakov, V.A. Stereoselective biotransformation of phenylglycine nitrile by heterogeneous biocatalyst based on immobilized bacterial cells and enzyme preparation. Dokl Biochem Biophys 474, 183–185 (2017). https://doi.org/10.1134/S1607672917030139
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DOI: https://doi.org/10.1134/S1607672917030139