Abstract
The taxonomic and functional structures of the actinomycetal complex in the litter and upper horizon of the brown forest soil was studied in a Pinus brutia var. pendulifolia forest on the eastern coast of the Aegean Sea. The complex of actinomycetes included representatives of the Streptomyces and Micromonospora genera and oligosporus forms. Streptomycetes predominated (73.8%) in the soil, and micromonospores (66.7%) were dominants in the litter. Thirty isolates of ten Streptomyces species from five series and three sections prevailed. In the upper soil horizon, species of the Helvolo-Flavus Helvolus section predominated (48%); the S. felleus species occurred most frequently. Among the isolated cultures, the S. globisporus and S. sindenensis species capable to produce antitumor antibiotics were found. The testing of the antimicrobial activity of the natural isolates showed that five strains inhibit the growth of pathogenic Fusarium sp., Alternaria sp., Acremonium sp., and Bipolaris sorokiniana fungi. When testing the effect of streptomycetes on the production of cellulases, a high-efficient strain belonging to the S. noboritoensis species was revealed. All the streptomycetes isolated from the brown forest soil produced auxins at the rate of 7.8 to 19.7 μg of indole acetic acid/mL of the liquid medium in the presence of 200 mg/L of tryptophan. Twelve isolates of streptomycetes were transferred to the collection of biotechnologically promising cultures for studying their properties.
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G. F. Gauze, T. P. Preobrazhenskaya, M. A. Sveshnikova, L. P. Terekhova, and T. S. Maksimova, Guide for Identification of Actinomyces. The Sreptomyces, Streptoverticillium, and Chainia Genera (Nauka, Moscow, 1983) [in Russian].
N. S. Egorov, Fundamentals of Science about Antibiotics (Vysshaya Shkola, Moscow, 1979) [in Russian].
D. G. Zvyagintsev and G. M. Zenova, Ecology of Actinomyces (GEOS, Moscow, 2001) [in Russian].
G. M. Zenova, Soil Actinomyces from Rare Genera: Methodological Guidelines (Moscow State Univ., Moscow, 2000) [in Russian].
G. M. Zenova, T. A. Gracheva, N. A. Manucharova, and D. G. Zvyagintsev, “Actinomyces communities in forest ecosystems,” Eurasian Soil Sci. 29, 1256–1259 (1996).
A. G. Isachenko and A. A. Shlyapnikov, The World Nature: Landscapes (Mysl’, Moscow, 1989) [in Russian].
O. V. Kokorina and I. G. Shirokikh, “Streptomyces in soils of national forest park in Chanchun city (northeast of China),” in Mechanism of Resistance and Adaptation of Biological Systems to Natural and Technogenic Factors (Vesi, Kirov, 2015), pp. 110–112.
Bergey’s Manual of Determinative Bacteriology, Ed. by J. G. Holt, N. R. Krieg, P. H. A. Sneath, and J. T. Staley (Williams and Wilkins, Baltimore, 1994; Mir, Moscow, 1997).
E. A. Tsavkelova, S. Yu. Klimova, T. A. Cherdyntseva, and A. I. Netrusov, “Microbial producers of plant growth stimulators and their practical use: a review,” Appl. Biochem. Microbiol. 42 (2), 117–126 (2006).
Yu. D. Shenin, I. I. Novikova, G. V. Kaminskii, and I. A. Ivanova, “Alirinomycin C is a novel macrolide antibiotic from Streptomyces felleus S-8 VIZR II,” Antibiot. Khimioter. 46 (2), 10–16 (2001).
I. G. Shirokikh, E. V. Tovstik, and Ya. I. Nazarova, “Prospects for creation of new agricultural biotechnologies and biopreparations based on Vyatka strains of Actinomyces,” Proceedings of the XI International Scientific-Practical Conference daRostim 2015 “Theory, Practice, and Prospects of Biologically Active Compounds in Agriculture,” Syktyvkar, June 17–19, 2015 (Institute of Chemistry, Komi Science Center, Ural Branch, Russian Academy of Sciences, Syktyvkar, 2015), pp. 219–222.
S. C. Aksoy and A. Uzel, “Extracellular serine proteases produced by Thermoactinomyces strains from hot springs and soils of West Anatolia,” Ann. Microbiol. 62 (2), 483–492 (2012).
A. L. Demain, “Small bugs, big business: the economic power of the microbe,” Biotechnol. Adv. 18 (6), 499–514 (2000).
A. L. Demain and A. Fang, “Emerging concepts of secondary metabolism in actinomycetes,” Actinomycetologia 9, 98–117 (1995).
J. P. Euzéby, List of prokaryotic names with standing in nomenclature, 2009. http://www.bacterio.net.
J. George, R. Arunachalam, K. Paulkumar, E. Wesely, S. Shiburaj, and G. Annadurai, “Characterization and phylogenetic analysis of cellulase producing S. noboritoensis SPKC1,” Interdiscip. Sci.: Comput. Life Sci. 2 (2), 205–212 (2010).
E. E. Hames-Kocabas and A. Uzel, “Alkaline protease production by an actinomycete MA1-1 isolated from marine sediments,” Ann Microbiol. 57, 71–75 (2007). http://springerlink.bibliotecabuap.elogim.com/article/10.1007/BF03175053.
T. Kaur, A. Vasudev, S. K. Sohal, and R. K. Manhas, “Insecticidal and growth inhibitory potential of Streptomyces hydrogenans DH16 on major pest of India, Spodoptera litura (Fab.) (Lepidoptera: Noctuidae),” BMC Microbiol. 14 (1), 1 (2014). doi 10.1186/s12866-014-0227-1
H. Korkmaz, M. N. Unaldi, B. Aslan, G. Coral, B. Arikan, S. Dincer, and O. Colak, “Keratinolytic activity of Streptomyces strain BA7, a new isolate from Turkey,” Ann. Microbiol. 53, 85–93 (2003).
E. Libbert and H. Risch, “Interactions between plants and epiphytic bacteria regarding their auxin metabolism. V. Isolation and identification of the IAA-producing and destroying bacteria from pea plants,” Physiol. Plant. 22, 51–58 (1969).
N. Myers, R. A. Mittermeier, C. G. Mittermeier, G. A. Da Fonseca, and J. Kent, “Biodiversity hotspots for conservation priorities,” Nature 403 (6772), 853–858 (2000).
A. M. Oskay, T. Üsame, and A. Cem, “Antibacterial activity of some actinomycetes isolated from farming soils of Turkey,” Afr. J. Biotechnol. 3 (9), 441–446 (2004). http://www.academicjournals.org/AJB.
K. Özcan, S. Ç. Aksoy, O. Kalkan, A. Uzel, E. E. Hames-Kocabas, and E. Bedir, “Diversity and antibiotic-producing potential of cultivable marine-derived actinomycetes from coastal sediments of Turkey,” J. Soils Sediments 13 (8), 1493–1501 (2013). doi 10.1007/s11368-013-0734-y
F. E. Sarigullu, E. Karadeniz, I. Untac, and O. Çolak, “Determination of antibacterial activities of isolated Streptomyces strains from soil at Çukurova University in Turkey,” J. Food Agric Environ. 11 (2), 922–924 (2013).
A. Sazak, N. Sahin, M. Camas, K. Guven, D. Cetin, and M. Goodfellow, “Streptosporangium anatoliense sp. nov., isolated from soil in Turkey,” Antonie Leeuwenhoek 102, 269–276 (2012). doi 10.1007/s10482-012-9735-x
H. Sebastian and M. J. Martinez, US Patent 3 864 480, 1975.
W. R. Strohl, “Antimicrobials,” in Microbial Diversity and Bioprocessing, Ed. by A. T. Bull (American Society for Microbiology, Washington, 2004), pp. 336–355.
S. Takeda, K. Yaji, K. Matsumoto, T. Amamoto, M. Shindo, and H. Aramaki, “Xanthocidin derivatives as topoisomerase IIa enzymatic inhibitors,” Biol. Pharm. Bull. 37 (2), 331–334 (2014).
R. M. Teather and P. J. Wood, “Use of Congo-red polysaccharide interaction in enumeration and characterization of cellulolytic bacteria the bovine rumen,” Appl. Environ. Microbiol. 43, 777–780 (1982).
M. Tuncer, A. Kuru, M. Isikli, N. Sahinand, and F. G. Celenk, “Optimization of extracellular endoxylanase, endoglucanase and peroxidase production by Streptomyces sp. F2621 isolated in Turkey,” J. Appl. Microbiol. 97 (4), 783–791 (2004). doi 10.1111/j.1365-2672.2004.02361.x
M. Yamaç, K. Isik, and N. Sahin, “Numerical classification of streptomycetes isolated from karstic caves in Turkey,” Turk J. Biol. 35, 473–484 (2011). doi 10.3906/biy-0911-185
S. Yücel and M. Yamaç, “Selection of Streptomyces isolates from Turkish karstic caves against antibiotic resistant microorganisms,” Pak. J. Pharm. Sci. 23 (1), 1–6 (2010).
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Original Russian Text © I.G. Shirokikh, A.A. Shirokikh, 2017, published in Pochvovedenie, 2017, No. 11, pp. 1335–1361.
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Shirokikh, I.G., Shirokikh, A.A. Biosynthetic potential of actinomycetes in brown forest soil on the eastern coast of the aegean sea. Eurasian Soil Sc. 50, 1311–1317 (2017). https://doi.org/10.1134/S1064229317110114
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DOI: https://doi.org/10.1134/S1064229317110114