Abstract
The relationship between the contents of abnormal erythroid forms (dacryocytes, nuclear invagination, and erythrocyte ghosts) and the number of immature erythrocytes (pronormoblasts, as well as basophilic and polychromatophilic normoblasts) was studied in the circulating blood of round gobies. It was found that the proliferative activity of the erythroid branch of blood-forming tissue in this species increases as the gonads mature, i.e., during transition from the III–IV to the V and VI–II stages of gonad maturity. The content of poorly differentiated cells in the blood grew by 26%, while the number of abnormal erythrocytes (dacryocytes and nuclear invagination) decreased by 3 or 4 times (R 2 > 0.95). The content of erythrocyte ghosts, in contrast, increased by 80%. When evaluating the toxicity level of the marine environment, not only the number of abnormal erythrocytes in fish blood but also the activity of the blood-forming tissue should be taken into account, as it substantially varies during the spawning period.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bulgakov, N.G., Indication of the state of natural ecosystems and normalization of environmental factors. An overview of the existing approaches, Usp. Sovrem. Biol., 2002, vol. 122, no. 2, pp. 115–135.
Davydov, O.N., Patologiya krovi ryb (Fish Blood Pathology), Kyiv: INKOS, 2006.
Zolotnitskaya, R.P., Methods of hematological studies, in Laboratornye metody issledovaniya v klinike (spravochnik) (Laboratory Methods of Research in Clinic, Reference Book), Moscow: Meditsina, 1987, pp. 106–148.
Kalaida, M.L., Nigmetzyanova, M.V., and Borisova, S.D., Obshchaya gistologiya i embriologiya ryb (Common Histology and Embryology of Fish), St. Petersburg: Prospekt Nauki, 2011.
Karapetyan, O.Sh., Tsema, N.I., and Dudkin, S.I., Molecular biomarkers of anthropogenic pollution in liver of the round goby, Neogobius melanosotomus, from the Taganrog Bay, Vopr. Rybolov., 2011, vol. 12, no. 4 (48), pp. 747–759.
Kutsenko, S.A., Osnovy toksikologii (Basic Toxicology), Moscow: Foliant, 2004.
Maslova, M.N. and Tavrovskaya, T.V., Dynamics of seasonal variations in the red blood system of lower vertebrates: seasonal dynamics of erythropoiesis in the trout Salmo gairdneri, Zh. Evol. Biokhim. Fiziol., 1991, vol. 27, pp. 796–798.
Miseiko, G.N., Bezmaternykh, D.M., and Tushkova, G.I., Biologicheskii analiz kachestva presnykh vod (Biological Analysis of the Quality of Fresh Waters), Barnaul: Altai. Gos. Univ., 2001.
Opekunova, M.G., Bioindikatsiya zagryaznenii (Bioindication of Pollutions), St. Petersburg: S.-Peterb. Gos. Univ., 2004.
Soldatov, A.A., Peculiarities of organization and functioning of the fish red blood system, J. Evol. Biochem. Physiol., 2005, vol. 41, no. 3, pp. 272–281.
Soldatov, A.A., Erythropoiesis and concentration of methemoglobin in the blood of golden gray mullet (Liza aurata Risso) during its annual cycle, in Sovremennye problemy fiziologii i biokhimii vodnykh organizmov (Modern Problems of Physiology and Biochemistry of Aquatic Organisms), Petrozavodsk: Inst. Biol. Karel. Nauch. Tsentr., Ross. Akad. Nauk, 2005, pp. 182–187.
Soldatov, A.A., Pashkova, E.V., and Kukhareva, T.A., Micronuclei inclusions in erythrocytes of round goby at various intensity of erythropoietic processes, Gidrobiol. Zh., 2012, vol. 48, no. 4, pp. 75–80.
Hematologic Pathophysiology, Schiffman, F.J., Ed., Philadelphia: Lippincott-Raven, 1998.
Auletta, A.E., Dearfield, K.L., and Cimino, M.C., Mutagenicity test schemes and guidelines: US EPA Office of Pollution Prevention and Toxics and Office of Pesticide Programs, Environ. Mol. Mutagen., 1993, vol. 21, pp. 38–45.
Ergene, S., Cavas, T., Celik, A., et al., Monitoring of nuclear abnormalities in peripheral erythrocytes of three fish species from the Goksu Delta (Turkey): genotoxic damage in relation to water pollution, Ecotoxicology, 2007, vol. 16, pp. 385–390.
Esteban, M.A., Meseguer, J., Ayala, G.A., and Agulleiro, B., Erythropoiesis and thrombopoiesis in the head-kidney of the sea bass: an ultrastructural study, Arch. Histol. Cytol., 1989, vol. 52, no. 4. pp. 407–419.
Ochiai, A., Ogawa, M., Umeda, S., and Taniguchi, N., Change of blood properties of maturing Japan eel at hormonal influences, Bull. Jpn. Soc. Sci. Fish., 1975, vol. 41, no 6, pp. 609–614.
Pottinger, T.G. and Pickering, A.D., Androgen levels and erythrocytosis in maturing brown trout, Salmo trutta L., Fish Physiol. Biochem., 1987, vol. 3, pp. 121–126.
Ranzani-Paiva, M.J.T., Hematological characteristics of the mullet, Mugil platanus G. from Cananeia lagoonestuarine region, Bol. Inst. Pesca-São-Paulo, 1995, vol. 22, pp. 1–22.
Strunjak-Perovic, I., Coz-Racovac, R., Topic-Popovic, N., and Jadan, M., Seasonality of nuclear abnormalities in gilthead sea bream Sparus aurata (L.) erythrocytes, Fish Physiol. Biochem., 2009, vol. 35, pp. 287–295.
Van der Oost, R.V., Beyer, J., and Vermeulen, N.P.E., Fish bioaccumulation and biomarkers in environmental risk assessment: a review, Environ. Toxicol. Pharmacol., 2003, vol. 13, pp. 57–149.
Wickramasinghe, S.N., Erythropoietin and the human kidney: evidence for an evolutionary link from studies of Salmo gairdneri, Comp. Biochem. Physiol., Part A: Mol. Integr. Physiol., 1993, vol. 104, pp. 63–65.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.A. Soldatov, T.A. Kukhareva, 2015, published in Biologiya Morya.
Rights and permissions
About this article
Cite this article
Soldatov, A.A., Kukhareva, T.A. Erythropoiesis and the contents of abnormal erythroid forms in the blood of the round goby, Neogobius melanostomus Pallas, 1811 (Osteichthyes: Gobiidae). Russ J Mar Biol 41, 315–320 (2015). https://doi.org/10.1134/S1063074015020121
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1063074015020121