Abstract
o-Phenylphenol is generally utilized as a disinfectant for citrus fruits. The purpose of this study is to develop a high-performance liquid chromatography coupled with ultraviolet detection (380 nm) method for simultaneous determination of o-phenylphenol, and its analogues (m-phenylphenol and p-phenylphenol) in grapefruit juice after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). 2-Hydroxyfluorene was used as an internal standard (IS). Standard curves were obtained after derivatization with NBD-F in borate buffer (pH 8.0) at room temperature for 5 min. The three NBD-F derivatives were almost completely separated on a Cholester column (5 μm, 3.0 mm i.d. × 150 mm). Calibration plots were linear in the range of absolute amount of 1.04 ∼ 2.08 to 41.6 ng/50 μL injection volume, with r 2 values ≥0.9981, for the three compounds. The lower limits of detection were 0.3 to 0.7 ng/50 μL injection volume (signal-to-noise ratio of 3 : 1). The coefficients of variation were less than 11.1%. After extraction of grapefruit juice (2.0 mL) with n-pentane, the level of o-phenylphenol in the juice was estimated to be 20.2 ± 2.0 ng/mL (n = 6, mean ± SD), while m-phenylphenol and p-phenylphenol were below the lower limits of quantification. The recovery values of the three phenylphenols from samples spiked with a standard mixture of authentic compounds and IS were satisfactory (99.1 to 118.7%).
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Davoren, M. and Fogarty, A.M., Toxicology In Vitro, 2007, vol. 20, no. 7, p. 1190.
Kolbe, N. and Andersson, J.T., J. Agric. Food Chem., 2006, vol. 54, no. 16, p. 5736.
Bomhard, E.M., Brendler-Schwaab, S.Y., Freyberger, A., Herbold, B.A., Leser, K.H., and Richter, M., Crit. Rev. Toxicol., 2002, vol. 32, no. 6, p. 551.
Thompson, R.D., J. AOAC Int., 2001, vol. 84, no. 3, p. 815.
Yang, L., Kotani, A., Hakamata, H., and Kusu, F., Anal. Sci., 2004, vol. 20, no. 1, p. 199.
WHO Guidelines for drinking-water quality incorporating first addendum. 2006, vol. 1, Recommendations. 3rd ed., p. 427.
Powis, G., Moore, D.J., Wilke, T.J., and Santone, K.S., Anal. Biochem., 1987, vol. 167, no. 1, p.191.
Imai, K., Yakugaku Zasshi, 2003, vol. 123, no. 11, p. 901.
Fukushima, T., Kawai, J., Imai, K., and Toyo’oka, T., Biomed. Chromatogr., 2004, vol. 18 no. 10, p. 813.
Higashi, Y., Nakamura, S., Matsumura, H., and Fujii, Y., Biomed. Chromatogr., 2006, vol. 20, no. 5, p. 423.
Higashi, Y., Sakata, M., and Fujii, Y., J. Liq. Chromatogr. & Rel. Technol., 2007, vol. 30, no. 18, p. 2747.
Higashi, Y., Gao, R., and Fujii, Y., J. Liq. Chromatogr. & Rel. Technol., 2009, vol. 32, no. 7, p. 1141.
Toyo’oka, T., Mantani, T., and Kato, M., Biomed. Chromatogr., 2003, vol. 17, nos. 2–3, p. 133.
Higashi, Y. and Fujii, Y., J. Liq. Chromatogr. & Rel. Technol., 2009, vol. 32, no. 16, p. 2372.
Higashi, Y. and Fujii, Y., J. Liq. Chromatogr. & Rel. Technol., 2011, vol. 34, no. 1, p. 18.
Yamazaki, Y. and Ninomiya, T., J. AOAC Int., 1999, vol. 82, no. 6, p. 1474.
Blasco, C., Picó, Mañes, J., and Font, G., J. Chromatogr. A, 2002, vol. 947, no. 2, p. 227.
Author information
Authors and Affiliations
Corresponding author
Additional information
The article is published in the original.
Rights and permissions
About this article
Cite this article
Higashi, Y., Fujii, Y. Determination of three phenylphenols in grapefruit juice by HPLC after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole. J Anal Chem 70, 346–350 (2015). https://doi.org/10.1134/S1061934815030235
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934815030235