Abstract
An analytical method for the simultaneous determination of 12 flavonol glycosides in buckwheat, black tea, and wild parsley using ultra high-performance liquid chromatography (UHPLC) coupled with a simple liquid extraction method using dimethyl sulfoxide (DMSO) was validated in precision, accuracy, and linearity. The UHPLC separation of target compounds was performed on a C18 column using a photodiode array (PDA) detector and the wavelength was fixed at 350 nm. The recovery values for flavonol glycosides ranged from 85.44 to 108.79%. The limits of detection and limits of quantification were less than 0.32 mg/kg and less than 0.97 mg/kg, respectively. The intraday and interday precisions were less than 13.69% for all the test samples. This method coupled with UHPLCPDA detection could be expected to provide more convenient sample preparation than conventional methods in the tested foods.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Wang ZM, Zhao D, Nie ZL, Zhao H, Zhou B, Gao W, Wang LS, Yang ZJ. Flavonol intake and stroke risk: A meta-analysis of cohort studies. Nutrition 30: 518–523 (2014)
Mouri C, Mozaffarian V, Zhang X, Laursen R. Characterization of flavonols in plants used for textile dyeing and the significance of flavonol conjugates. Dyes Pigments 100: 135–141 (2014)
Chirumbolo S. The role of quercetin, flavonols and flavones in modulating inflammatory cell function. Inflamm. Allergy Drug Targets 9: 263–285 (2010)
Lee SJ, Son KH, Chang HW, Do JC, Jung KY, Kang SS, Kim HP. Antiinflammatory activity of naturally occurring flavone and flavonol glycosides. Arch. Pharm. Res. 16: 25–28 (1993)
Verma AK, Pratap R. Chemistry of biologically important flavones. Tetrahedron 68: 8523–8538 (2012)
Murakami A, Ashida H, Terao J. Multitargeted cancer prevention by quercetin. Cancer Lett. 269: 315–325 (2008)
Hollman PCH, Geelen A, Kromhout D. Dietary flavonol intake may lower stroke risk in men and women. J. Nutr. 140: 600–604 (2010)
Özçelik B, Orhan I, Toker G. Antiviral and antimicrobial assessment of some selected flavonoids. Z. Naturforsch C 61: 632–638 (2006)
Beschia M, Leonte A, Oencea I. Phenolic compounds with biological activity. Bull. Univ. Galati Faso. 6: 23–27 (1984)
Shim YS, Kim S, Seo D, Ito M, Nakagawa H, Park HJ, Ha J. Rapid method for the simultaneous determination of flavonol aglycones in food using u-HPLC coupled with heating block acidic hydrolysis. J. AOAC Int. 96: 1059–1064 (2013)
Liu XG, Yang H, Cheng XL, Liu L, Qin Y, Wang Q, Qi LW, Li P. Direct analysis of 18 flavonol glycosides, aglycones and terpene tilactones in Gingko biloba tablets by matrix solid phase dispersion coupled with ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry. J. Pharmaceut. Biomed. 97: 123–128 (2014)
Liu P, Kallio H, Yang B. Flavonol glycosides and other phenolic compounds in buds and leaves of different varieties of black currant (Ribes nigrum L.) and changes during growing season. Food Chem. 160: 180–189 (2014)
Zhang H, Yang H, Zhang M, Wang Y, Wang J, Yau L, Jiang Z, Hu P. Identification of flavonol and triterpene glycosides in Luo-Han-Guo extract using ultra-high performance liquid chromatography/quadrupole time of-flight mass spectrometry. J. Food Compos. Anal. 25: 142–148 (2012)
Vrhovsek U, Mauero D, Palmieri P, Mattivi F. Identification and quantification of flavonol glycosides in cultivated blueberry cultivars. J. Food Compos. Anal. 25: 9–16 (2012)
Thabti I, Elfalleh W, Hannachi H, Ferchichi A, Campos MDG. Identification and quantification of phenolic acids and flavonol glycosides in Tunisian Morus species by HPLC-DAD and HPLC-MS. J. Funct. Foods. 4: 367–374 (2012)
Vallabhaneni P, Ray WK, Winkel BSJ, Helm RF. Characterization of flavonol glycosides in individual Arabidopsis root tips by flow injection electrospray mass spectrometry. Phytochemistry 73: 114–118 (2012)
Tantry MA, Akbar S, Dar JA, Irtiza S, Galal A, Khuroo MA, Ghazanfar K. Acylated flavonol glycoside from Platanus orientalis. Fitoterapia 83: 281–285 (2012)
Gossan DPA, Magid AA, Yao-Kouassi PA, Coffy AA, Harakat D, Voutquenne-Nazabadioko L. New acylated flavonol glycosides from the aerial parts of Gouania longipetala. Phytochem. Lett. 11: 306–310 (2015)
Chen C, Xu XM, Chen Y, Yu MY, Wen FY, Zhang H. Identification, quantification and antioxidant activity of acylated flavonol glycosides from sea buckthorn (Hippophae rhamnoides ssp. sinensis). Food Chem. 141: 1573–1579 (2013)
ICH. Guidance for Industry, Q2B Validation of Analytical Procedures: Methodology. ICH-Q2B, Kuala Lumpur, Malaysia. pp.71–76 (1996)
Ahn H, Kim J, Jeung EB, Lee GS. Dimethyl sulfoxide inhibits NLRP3 inflammaxome activation. Immunobiology 219: 315–322 (2014)
Shim YS, Kim S, Seo D, Park HJ, Ha J. Rapid method for determination of anthocyanin glycosides and free delphinidin in grapes using u-HPLC. J. Chromtogr. Sci. 52: 629–635 (2014)
Shim YS, Yoon WJ, Hwang JB, Park HJ, Seo D, Ha J. Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photodiode array detection. Food Chem. 187: 391–397 (2015)
Hollebeeck S, Raas T, Piront N, Schneider YJ, Toussaint O, Larondelle Y, During A. Dimethyl sulfoxide (DMS) attenuates the inflammatory response in the in vitro intestinal Caco-2 cell model. Toxicol. Lett. 206: 268–275 (2011)
Maeng YS, Park HK, Kwon TB. Analysis of rutin contents in buckwheat and buckwheat foods. Korean J. Food Sci. Biotechnol. 22: 732–737 (1990)
US Department of Health and Human Services (DHHS). Center for Veterinary Medicine (CVM): Guidance for Industry, Bioanalytical Method. Center for Drug Evaluation and Research (CDER), Food and Drug Administration, US department of Health and Human Services, Washington, DC, USA (2001)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, JC., Shim, YS. Method validation of analytical method for 12 flavonol glycosides in foods using ultra high-performance liquid chromatography coupled with photodiode array detection. Food Sci Biotechnol 25, 659–664 (2016). https://doi.org/10.1007/s10068-016-0116-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10068-016-0116-5