Abstract
Fatty acid (FA) composition of total lipids in embryogenic and nonembryogenic calli of Siberian larch (Larix sibirica Ledeb.) was investigated by the method of GC-MS. We detected a high content of oleic acid in total lipids of embryogenic cell culture (32–56% by weight of total FA), which apparently depends on a high activity of acyl carrier protein (stearoyl-ACP-Δ9-desat-urase). At the same time, activity of Δ12-desaturase in the cells of embryogenic calli was considerably (2–3 times) lower than in nonembryogenic calli. We discuss a possibility that concentration of FA (oleic and linoleic acids) may be used as a marker of embryogenic potential when promising cell lines of Siberian larch are screened in the stage of early embryogenesis.
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Abbreviations
- ACR:
-
acyl carrier protein
- EM:
-
embryonal mass
- FAME:
-
fatty acid methyl esters
- PUFA:
-
polyunsaturated fatty acids
- UI:
-
unsaturation index
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Original Russian Text © S.P. Makarenko, V.N. Shmakov, L.V. Dudareva, A.V. Stolbikova, N.V. Semenova, I.N. Tret’yakova, Yu.M. Konstantinov, 2016, published in Fiziologiya Rastenii, 2016, Vol. 63, No. 2, pp. 267–274.
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Makarenko, S.P., Shmakov, V.N., Dudareva, L.V. et al. Fatty acid composition of total lipids in embryogenic and nonembryogenic callus lines of larch. Russ J Plant Physiol 63, 252–258 (2016). https://doi.org/10.1134/S1021443716020102
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DOI: https://doi.org/10.1134/S1021443716020102