Abstract
Ethylene, being one of five classical plant phytohormones is involved in regulation of numerous physiological processes. There are contradictory data about the effect of ethylene on the cell growth and division; although it is accepted that in culture flasks, the content of ethylene rises to a few tens of μL/L and production of ethylene is associated with the periods of active growth of the cells in vitro. We revealed a strong correlation (r = 0.96) between ethylene production and specific rate of dry weight accumulation in suspension cell cultures of Ajuga turkestanica, heterotrophic and mixotrophic strains of Arabidopsis thaliana, Beta vulgaris, Euonymus maximoviczianus, Medicago sativa, Panax ginseng, and Triticum timopheevii. In heterotrophic cell culture of A. thaliana, the peaks and general shape of the curves describing dynamics of ethylene production, the number of S-phase cells, and specific rate of increase in cell number coincided in log phase and in the phase of growth deceleration. Pretreatment of subculture inoculum with 100 μL/L ethylene caused doubling of S-phase cell number after 3-h-long culturing in fresh nutrient medium. It was found that exogenous ethylene affects the number of S-phase cells only when the level of endogenously produced ethylene is low.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- CC:
-
cell cycle
- EdU:
-
5-ethynyl-2’-deoxyuridine
- ERF:
-
ethylene response factors
- PBS:
-
phosphate buffered saline
- SH:
-
Schenk and Hildebrandt medium
References
Neljubow, D., Über die horizontale Nutation der Stengel von Pisum sativum und einiger anderer Pflanzen, Beih. Bot. Zentralbl., 1901, vol. 10, pp. 128–138.
Abeles, F.B., Morgan, P.W., and Salveit, M.E., Ethylene in Plant Biology, San Diego: Academic, 1992.
McManus, M.T., The Plant Hormone Ethylene, vol. 44, Annual Plant Reviews, Oxford: Wiley, 2012.
Bleecker, A.B., Estelle, M.A., Somerville, C., and Kende, H., Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana, Science, 1988, vol. 241, pp. 1086–1089.
Guzman, P. and Ecker, J.R., Exploiting the triple response of Arabidopsis to identify ethylene-related mutants, Plant Cell, 1990, vol. 2, pp. 513–523.
Grierson, D., 100 years of ethylene a personal view, in The Plant Hormone Ethylene, vol. 44, Annual Plant Reviews, Wiley Online Library, 2012, pp. 1–17, do. 10.1002/9781118223086.ch.
Ju, C. and Chang, C., Advances in ethylene signaling: protein complexes at the endoplasmic reticulum membrane, AoB Plants, 2012: pls031, do. 10.1093/aobpla/ pls03.
Novikova, G.V., Moshkov, I.E., Smith, A.R., and Hall,M.A., The effect of ethylene on MAPKinase-like activity in Arabidopsis thaliana, FEBS Lett., 2000, vol. 474, pp. 29–32.
Zhang, J., Yu, J., and Wen, C.K., An alternate route of ethylene receptor signaling, Front. Plant Sci., 2014, vol. 5. do. 10.3389/fpls.2014.0064.
Cho, Y.H. and Yoo, S.D., Novel connections and gaps in ethylene signaling from the ER membrane to the nucleus, Front. Plant Sci., 2015, vol. 5. do. 10.3389/ fpls.2014.0073.
Shakeel, S.N., Gao, Z., Amir, M., Chen, Y.F., Rai, M.I., Haq, N.U., and Schaller, G.E., Ethylene regulates levels of ethylene receptor/CTR1 signaling complexes in Arabidopsis thaliana, J. Biol. Chem., 2015, vol. 290, pp. 12 415–12 424.
Pierik, R., Tholen, D., Poorter, H., Visser, E.J.W., and Voesenek, L.A.C.J., The Janus face of ethylene: growth inhibition and stimulation, Trends Plant Sci., 2006, vol. 11, pp. 176–183.
Polko, J.K., Voesenek, L.A.C.J., Peeters, A.J.M., and Pierik, R., Petiole hyponasty: an ethylene-driven, adaptive response to changes in the environment, AoB Plants, p. 2011, plr031. do. 10.1093/aobpla/plr03.
Apelbaum, A. and Burg, S.P., Effect of ethylene on celldivision and deoxyribonucleic acid synthesis in Pisum sativum, Plant Physiol., 1972, vol. 50, pp. 117–124.
Herbert, R.J., Vilhar, B., Evett, C., Orchard, C.B., Rogers, H.J., Davies, M.S., and Francis, D., Ethylene induces cell death at particular phases of the cell cycle in the tobacco TBY-2 cell line, J. Exp. Bot., 2001, vol. 52, pp. 1615–1623.
Dan, H., Imaseki, H., Wasteneys, G.O., and Kazama, H., Ethylene stimulates endoreduplication but inhibits cytokinesis in cucumber hypocotyl epidermis, Plant Physiol., 2003, vol. 133, pp. 1726–1731.
Ortega-Martínez, O., Pernas, M., Carol, R.J., and Dolan, L., Ethylene modulates stem cell division in the Arabidopsis thaliana root, Science, 2007, vol. 317, pp. 507–510.
Love, J., Björklund, S., Vahala, J., Hertzberg, M., Kangasjärvi, J., and Sundberg, B., Ethylene is an endogenous stimulator of cell division in the cambial meristem of Populus, Proc. Natl. Acad. Sci. USA, 2009, vol. 106, pp. 5984–5989.
Bystrova, E.I., Zhukovskaya, N.V., Rakitin, V.Yu., and Ivanov, V.B., Role of ethylene in activation of cell division in quiescent center of excised maize roots, Russ. J. Dev. Biol., 2015, vol. 46, pp. 60–64.
Nagata, T., Nemoto, Y., and Hasezawa, S., Tobacco BY-2 cell line as the ‘HeLa’ cell in the cell biology of higher plants, Int. Rev. Cytol., 1992, vol. 132, pp. 1–30.
Biddington, N.L., The influence of ethylene in plant tissue culture, Plant Growth Regul., 1992, vol. 11, pp. 173–187.
Moshkov, I.E., Novikova, G.V., Hall, M.A., and George, E.F., Plant growth regulators. III. Gibberellins, ethylene, abscisic acid, their analogues and inhibitors; miscellaneous compounds, in Plant Propagation by Tissue Culture, vol. 1, The Background, George, E.F., Hall, M.A., and De Klerk, G.-J., Eds., Dordrecht: Springer-Verlag, 2008, pp. 227–281.
LaRue, T.A.G. and Gamborg, O.L., Ethylene production by plant cell cultures. Variations in production during growing cycle and in different plant species, Plant Physiol., 1971, vol. 48, pp. 394–398.
Fomenkov, A.A., Nosov, A.V., Rakitin, V.Yu., Mamaeva, A.S., and Novikova, G.V., Cytophysiological characteristics of Arabidopsis thaliana cultivated cells with disable perception of ethylene signal by the ETR1 receptor, Russ. J. Plant Physiol., 2014, vol. 61, pp. 598–607.
Nosov, A.V., Fomenkov, A.A., Mamaeva, A.S., Solovchenko, A.E., and Novikova, G.V., Extra perspectives of 5-ethynyl-2’-deoxyuridine click reaction with fluorochrome azides to study cell cycle and deoxyribonucleoside metabolism, Russ. J. Plant Physiol., 2014, vol. 61, pp. 899–909.
Rakitin, V.Yu. and Rakitin, L.Yu., Determination of gas exchange and contents of ethylene, CO2 and O2 in plant tissues, Sov. Plant Physiol., 1986, vol. 33, pp. 403–413.
Gould, A.R., Everett, N.P., Wang, T.L., and Street, H.E., Studies on the control of the cell cycle in cultured plant cells, Protoplasma, 1981, vol. 106, pp. 1–13.
Meir, S., Philosoph-Hadas, S., Epstein, E., and Aharoni, N., Carbohydrates stimulate ethylene production in tobacco leaf discs. I. Interaction with auxin and the relation to auxin metabolism, Plant Physiol., 1985, vol. 78, pp. 131–138.
Konze, J.R. and Kwiatkowski, G.M.K., Rapidly induced ethylene formation after wounding is controlled by the regulation of 1-aminocyclopropane-1carboxylic acid synthesis, Planta, 1981, vol. 151, pp. 327–330.
Mehrnia, M., Balazadeh, S., Zanor, M.I., and Mueller-Roeber, B., EBE, an AP2/ERF transcription factor highly expressed in proliferating cells, affects shoot architecture in Arabidopsis, Plant Physiol., 2013, vol. 162, pp. 842–857.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.A. Fomenkov, A.V. Nosov, V.Yu. Rakitin, E.S. Sukhanova, A.S. Mamaeva, G.I. Sobol’kova, A.M. Nosov, G.V. Novikova, 2015, published in Fiziologiya Rastenii, 2015, Vol. 62, No. 6, pp. 839–846.
Rights and permissions
About this article
Cite this article
Fomenkov, A.A., Nosov, A.V., Rakitin, V.Y. et al. Ethylene in the proliferation of cultured plant cells: Regulating or just going along?. Russ J Plant Physiol 62, 815–822 (2015). https://doi.org/10.1134/S1021443715060059
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1021443715060059