Abstract
The capacity of a recombinant Yarrowia lipolytica yeast strain VKPM Y3753 for succinic acid biosynthesis in a laboratory bioreactor at low pH has been studied. The batch and repeated-batch modes of fermentation of the strain were compared. The optimal conditions for repeated-batch fermentation were selected; they resulted in the accumulation of 55.3 g/L of succinic acid and the maximal productivity for this compound, 2.6 g/(L h), while lowering the pH of the broth culture to 3.65 at the end of the biosynthesis process.
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Abbreviations
- VKPM:
-
Russian National Collection of Industrial Microorganisms
- BC:
-
broth culture
- SA:
-
succinic acid.
References
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Original Russian Text © P.Yu. Bondarenko, A.S. Fedorov, S.P. Sineoky, 2016, published in Biotekhnologiya, 2016, Vol. 32, No. 6, pp. 68–75.
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Bondarenko, P.Y., Fedorov, A.S. & Sineoky, S.P. Optimization of Repeated-Batch Fermentation of a Recombinant Strain of the Yeast Yarrowia lipolytica for Succinic Acid Production at Low pH. Appl Biochem Microbiol 53, 882–887 (2017). https://doi.org/10.1134/S0003683817090022
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DOI: https://doi.org/10.1134/S0003683817090022