Abstract
Oil-degrading bacteria were isolated from soil and water samples taken in Russia, Kazakhstan, and the Antarctic; 13 of 86 strains proved to be thermotolerant. These bacteria utilized crude oil at 45–50°C; their growth optimum (35–37°C) and range (20–53°C) differ from those of mesophilic bacteria. Thermotolerant strains were identified as representatives of the genera Rhodococcus and Gordonia. It was shown that their ability to degrade petroleum products does not differ at 24 and 45°C. The strains Rhodococcus sp. Par7 and Gordonia sp. 1D utilized 14 and 20% of the oil, respectively, in 14 days at 45°C. All of the isolated thermotolerant bacteria grew in a medium containing 3% NaCl; the medium for the strains Gordonia amicalis 1B and Gordonia sp. 1D contained up to 10% NaCl. The bacteria G. amicalis and Rhodococcus erythropolis were able to utilize crude oil and individual hydrocarbons at higher (up to 50°C) temperatures.
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Zavarzin, G.A. and Kolotilova, N.N., Vvedenie v prirodovedcheskuyu mikrobiologiyu (Introduction to Natural Science Microbiology), Moscow: Knizhnyi dom “Universitet,” 2001.
Loginova, L.G. and Pozmogova, I.N., Zhizn’ rastenii (Life of Plants), Krasil’nikov, N.A. and Uranov, A.A, Eds., Moscow: Prosveshchenie, 1974, vol.1.
Gusev, M.V. and Mineeva, L.A., Mikrobiologiya (Microbiology), Moscow: Akademiya, 2003.
Marchant, R., Banat, I.M., Rahman, T.J., and Benzano, M., Trends Microbiol., 2002, vol. 10, no. 3, pp. 120–121.
Evans, C.G.T., Herbert, D., and Tempest, D.W., in Methods in Microbiology, Norris, J.R. and Ribbons, D.W., Eds., London: Acad. Press, 1970, pp. 277–327.
Miller, J.H., Experiments in Molecular Biology, New York: Cold Spring Harbor Laboratory, 1972.
Bertani, G., J. Bacteriol., 1951, vol. 62, no. 3, pp. 293–300.
Perth, S.J., Osnovy kul’tivirovaniya mikroorganizmov i kletok (Basics of Microorganism and Cell Cultivation), Rabotnova, I.L, Ed., Moscow: Mir, 1978.
Ausubel, F.M., in Current Protocols in Molecular Biology, Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., and Struhl, K., Eds., New York: John Wiley and Sons, 2003.
Marchesi, J.R., Sato, T., Weightman, A., Martin, T., Fry, J.C., Hiom, S.J., and Wade, W.G., Appl. Environ. Microbiol., 1998, vol. 64, no. 2, pp. 795–799.
Yamamoto, S. and Harayama, S., Appl. Environ. Microbiol., 1995, vol. 61, no. 3, pp. 1104–1109.
Shen, F.T., Young, L.S., Hsieh, M.F., Lin, S.Y., and Young, C.C., Syst. Appl. Microbiol., 2010, vol. 33, no. 2, pp. 53–59.
QIAquick PCR purification kit protocol, in QIAquick Spin Handbook, QIAGEN, 2008.
Stradomskaya, A.G., Boeva, L.G., and Ryazantseva, I.A., Massovaya kontsentratsiya nefteproduktov v vodakh. Metodika vypolneniya izmerenii IK-fotometricheskim metodom. RD 52.24.476-2007 (Mass Concentration of Oil Products in Water. Measurement Technique by Infrared Photometric Method), Rostov-on-Don: Gidrokhimicheskii institut, 2007.
Geiselbrecht, A.G., Herwig, R.P., Deming, J.W., and Staley, J.T., Appl. Environ. Microbiol., 1996, vol. 62, no. 9, pp. 3344–3349.
Imperio, T., Viti, C., and Marri, L., Int. J. Syst. Evol. Microbiol., 2008, vol. 58, no. 1, pp. 221–225.
Gousterova, A., Paskaleva, D., and Vasileva-Tonkova, E., Int. Res. J. Biol. Sci., 2014, vol. 3, no. 3, pp. 30–36.
Tancsics, A., Benedek, T., Farkas, M., Mathe, I., Marialigeti, K., Szoboszlay, S., Kukolya, J., and Kriszt, B., Int. J. Syst. Evol. Microbiol., 2014, vol. 64, no. 1, pp. 298–301.
Takei, D., Washio, K., and Morikawa, M., Biotechnol. Lett., 2008, vol. 30, no. 8, pp. 1447–1452.
Arenskotter, M., Broker, D., and Steinbuchel, A., Appl. Environ. Microbiol., 2004, vol. 70, no. 6, pp. 3195–3204.
Xue, Y., Sun, X., Zhou, P., Liu, R., Liang, F., and Ma, Y., Int. J. Syst. Evol. Microbiol., 2003, vol. 53, no. 5, pp. 1643–1646.
Hao, D., Lin, J.Q., Song, X., Su, Y.J., and Qu, Y.B., Biotechnol. Bioprocess Eng., 2008, vol. 13, no. 1, pp. 61–68.
Sorkhoh, N.A., Ghannoum, M.A., Stretton, R.J., and Radwan, S.S., Environ. Pollut., 1990, vol. 65, no. 1, pp. 1–17.
Abu-Ruwaida, A.S., Banat, I.M., Haditirto, S., Salem, A., and Kadri, M., Acta Biotechnol., 1991, vol. 11, no. 4, pp. 315–324.
Kayasth, M., Kumar, V., and Gera, R., 3 Biotech., 2014, vol. 4, no. 5, pp. 553–557.
Li, S.H., Jin, Y., Cheng, J., Park, D.J., Kim, C.J., Hozzein, W.N., Wadaan, M.A.M., Shu, W.S., Ding, L.X., and Li, W.J., Antonie van Leeuwenhoek J. Microbiol., 2014, vol. 106, no. 2, pp. 347–356.
Van Hamme, J.D., Singh, A., and Ward, O.P., Microbiol. Mol. Biol. Rev., 2003, vol. 67, no. 4, pp. 503–549.
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Original Russian Text © Ya.A. Delegan, A.A. Vetrova, V.N. Akimov, M.A. Titok, A.E. Filonov, A.M. Boronin, 2016, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2016, Vol. 52, No. 4, pp. 383–391.
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Delegan, Y.A., Vetrova, A.A., Akimov, V.N. et al. Thermotolerant oil-degrading bacteria isolated from soil and water of geographically distant regions. Appl Biochem Microbiol 52, 389–396 (2016). https://doi.org/10.1134/S0003683816040025
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DOI: https://doi.org/10.1134/S0003683816040025