Abstract
Polycomb-group (PcG) protein MULTICOPY SUPPRESSOR OF IRA1 (MSI1) protein is an evolutionarily conserved developmental suppressor and plays a crucial role in regulating epigenetic modulations. However, the potential role and function of MSI1 in fleshy fruits remain unknown. In this study, SlMSI1 was cloned and transformed into tomato to explore its function. The quantitative real-time PCR results showed that SlMSI1 was highly expressed in flowers and fruits and that its transcript and protein levels were significantly decreased in fruits after the breaker stage. Additionally, SlMSI1-overexpressing transgenic tomatoes displayed abnormal non-ripening fruit formation, whereas its suppression promoted fruit ripening in transgenic tomatoes. Quantitative real-time PCR assays also showed that RIN and its regulons were decreased in SlMSI1 overexpression transgenic tomato fruits. Furthermore, RNA-seq analysis demonstrated that SlMSI1 inhibits fruit ripening by negatively regulating a large set of fruit-ripening genes in addition to RIN and its regulons. Finally, genetic manipulation of SlMSI1 and RIN successfully prolonged the fruit shelf life by regulating the fruit-ripening genes in tomato. Our findings reveal a novel regulatory function of SlMSI1 in fruit ripening and provide a new regulator that may be useful for genetic engineering and modification of fruit shelf life.
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Introduction
Polycomb-group (PcG) proteins, which were first identified in Drosophila melanogaster, are evolutionarily conserved developmental suppressors and play a crucial role in regulating epigenetic modulations in plant and animal species1,2,3. These PcG proteins form distinct complexes to control vegetative and reproductive development and play important roles in phase transitions during development, cell fate determination and cellular differentiation by repressing sets of genes that regulate either proliferation or differentiation in plants4,5,6. The well-studied PcG complexes include the polycomb repressive complex 2 (PRC2) and PRC1, which are thought to act in a sequential manner to stably maintain gene repression4. PRC2 induces histone H3 trimethylation of lysine 27 (H3K27me3), which is sequentially read by PRC1 that catalyzes H2A monoubiquitination (H2Aub1) to stably repress target genes expression7. PRC1 and PRC2 complexes play a global role in mediating gene regulation networks during plant development.
Numerous PcG proteins have been purified. The components of PRC1 are considerably different between animals and plants, whereas the components of PRC2 are evolutionarily conserved. In Arabidopsis, there is only one PRC1 complex, which consists of LIKE HETEROCHROMATIN PROTEIN1 (LHP1), AtRING1A/AtRING1B, AtBMI1A/AtBMI1B, and EMBRYONIC FLOWER1 (EMF1) [probably VERTILIZATION 1 (VRN1)]. PRC1 functions to maintain H3K27me3, perform histone H2A monoubiquitination (H2Aub1), and/or condense chromatin to inhibit transcription8. The PRC2 complex in Drosophila is composed of four core elements: histone methyltransferase enhancer of zeste (E(z)), which has three homologous proteins, CURLY LEAF (CLF), SWINGER (SWN), MEDEA (MEA), in Arabidopsis; Suppressor of zeste 12 (Su(z)12), which has three homologous proteins, EMBRYONIC FLOWER 2 (EMF2), FERTILIZATION-INDEPDENT SEED 2 (FIS2) and VRN2, in Arabidopsis; extra sex comb protein (Esc), which has only one homologous protein, FERTILIZATION-INDEPENDENT ENDOSPERM (FIE), in Arabidopsis; and core protein p55, which has five homologous proteins, i.e., MULTICOPY SUPPRESSOR OF IRA 1–5 (MSI1-5), in Arabidopsis9. In addition, these proteins form three types of PRC2 complexes, i.e., FERTILIZATION INDEPENDENT SEED (FIS)-PRC2, VRN-PRC2, and EMF-PRC2. The FIS-PRC2 complex contains FIS1/MEA, FIS2, FIS3/MEA, and MSI1, which functions in regulating female gametophyte and endosperm development10,11. The VRN-PRC2 complex is composed of VRN2, FIS3/FIE, CLF (or SWN) and MSI1 and accelerates flowering in response to prolonged exposure to cold12,13. The EMF-PRC2 complex is composed of EMF2, FIS3/FIE, CLF (or SWN) and MSI1 and controls vegetative development and the transition to flowering9,14.
PcG proteins are evolutionarily conserved developmental suppressors and play a crucial role in regulating epigenetic modulations, i.e., DNA methylation and histone methylation, in plant and animal species1,3. In tomato, a global methylome analysis demonstrated that DNA methylation has crucial regulatory roles in tomato fruit development and maturation by controlling the timing of the ripening process15,16,17. The tomato is a popular model plant for studying fruit ripening18. In recent decades, investigations have focused on the identification of spontaneous ripening-deficient mutants and characterization of the increasing number of known ripening-related transcription factors (TFs)19,20. Of these known TFs, the MADS-box protein RIN (RIPENING INHIBITOR) appears to be a master regulator that is critical for the transcriptional regulation of ripening initiation and progression by directly binding to a conserved cis-element known as the C-(A/T)-rich-G (CArG) box in the promoter regions of itself and various other ripening genes21,22,23. Studies on RIN and its regulon have elucidated the complicated regulatory network of fruit ripening. Identification of the tomato epiallele gene CNR (COLOURLESS NON-RIPENING) has provided strong evidence for the possible role of epigenetic processes in fruit ripening24. Recently, increasing evidence has indicated that epigenetic modulations are involved in the control of fruit ripening. Some PcG genes, such as SlEZ125 and SlVIN326, have been found to regulate flower/fruit development and floral organ differentiation in tomato, and SlEZ227 has been shown to function in fruit development and ripening.
In Arabidopsis, MSI1 is an indispensable component of the PRC2 complex. A mutation in MSI1 affects vegetative development, transition to flowering and seed formation6,28. In tomato, it has been shown that SlMSI1 binds to a 65 kD protein during fruit ripening29. However, its function in fleshy fruits is largely unknown. In this study, SlMSI1 was identified as having a crucial function in fruit ripening. It repressed the expression of RIN and other fruit ripening genes. Additionally, the utilization of SlMSI1-mediated biotechnology in the genetic manipulation of fruit shelf life was investigated and discussed.
Results
SlMSI1 transcript and protein levels decrease with fruit ripening in tomato
Based on the sequence in the tomato genome (https://solgenomics.net/organism/Solanum_lycopersicum/genome), full-length cDNA of SlMSI1 (Solyc01g104510.2.1) was cloned from Alisa Craig tomato. To explore its tissue-specific expression profile, quantitative real-time PCR (qRT-PCR) and western blot assays were performed to determine the temporal and spatial patterns of SlMSI1 transcripts/proteins in tomato roots, stems, leaves, flowers and fruits at different ripening stages. The results showed that SlMSI1 was constitutively accumulated in all tested organs, especially in the flower and fruit (Fig. 1A,B), thus suggesting a potential role in reproductive development.
For fruits, the entire ripening process was divided into eight stages, which were indicated as the day after anthesis (dpa). The SlMSI1 transcript and protein levels were found to vary with fruit development and ripening (Fig. 1C,D). Both levels were very low during the early stages of fruit development and then dramatically increased up to a maximum level at 45 dpa. Subsequently, SlMSI1 transcript and protein levels gradually decreased with fruit ripening, especially after the breaker stages (Fig. 1C,D), suggesting that SlMSI1 is involved in the regulation of fruit ripening in tomato.
SlMSI1 affects fruit ripening in tomato
To characterize the function of SlMSI1 in planta, transgenic tomatoes containing pBIN-SlMSI1-GFP and empty vector pBIN-GFP were obtained. Three SlMSI1-GFP overexpression lines L1, L2 and L29 were chosen for further investigation, and a pBIN-GFP line was used as the control. The overexpression lines generated more transcripts and proteins of SlMSI1 than the control (Fig. 2A). Compared with the control plants, SlMSI1 overexpression transgenic lines displayed abnormal flowers with larger sepals and transgenic line L29 showed the indeterminacy inflorescences (Fig. 2B). Additionally, floral dissection revealed smaller stamens and pistils in the overexpression transgenic plants (Fig. 2C). Interestingly, all transgenic tomato fruits produced non-ripening fruits, even at the mature stage, which were highly similar to those in rin mutant tomato30 (Fig. 2D). Additionally, the overexpression transgenic fruits displayed increased pericarp firmness during the mature green, breaker and mature stages (Supplementary Fig. 1). These results suggest that SlMSI1 might play a novel role in fleshy fruit development.
Furthermore, a specific antisense SlMSI1 cDNA fragment was used to construct a suppression vector, which was then genetically transformed into tomato. Finally, 3 suppression lines, SL1, SL2, and SL3, were selected from among the 17 transgenic suppression lines for further investigation. The three lines showed markedly decreased SlMSI1 transcript and protein levels (Fig. 2E). As a result, the 3 suppression lines generated fruits that ripened earlier than the control fruits (Fig. 2F). Additionally, no other obvious changes were found during fruit development and post-harvest. Taken together, these finding suggest that SlMSI1 inhibits fruit ripening in tomato.
During fruit ripening, the tomato releases a high quantity of ethylene gas that ripens its fruits. We hypothesized that the SlMSI1 overexpression transgenic lines, which displayed non-ripening fruits, failed to produce ethylene (Fig. 2D). To verify our assumption, we measured ethylene production in the control and transgenic fruits at the breaker stage. The control fruits exhibited a rapid and strong increase in ethylene production; the ethylene level increased to a maximum value at 5 days after the breaker stage and then decreased suddenly until fruit ripening (Fig. 2F). In contrast, there were no obvious changes in the ethylene level in SlMSI1 overexpression transgenic tomato fruits during ripening (Fig. 2G). Furthermore, the overexpression transgenic tomato fruits failed to ripen when they were treated with exogenous ethylene. These results indicate that the climacteric of ethylene was absent in SlMSI1 overexpression transgenic fruits during ripening.
SlMSI1 inhibits fruit ripening by repressing the expression of RIN and its regulon genes
SlMSI1 overexpression transgenic tomatoes displayed enlarged sepals and non-ripening fruits that were highly similar to, and even more serious than, those of the rin mutant (Fig. 2D). It was assumed that SlMSI1 might inhibit ripening via repressed RIN expression. To test our hypothesis, semi-quantitative PCR was first performed using control and transgenic tomato fruits. This expression analysis demonstrated that RIN transcripts were markedly decreased in the three overexpression lines compared with the control (Fig. 3A). Additionally, no changes in the SlMSI1 transcript and protein levels were found in the rin mutant (Fig. 3B), suggesting that SlMSI1 might act upstream of RIN in regulating fruit ripening.
In tomato, RIN appears to be a master regulator that is critical for the transcriptional regulation of ripening initiation and progression21,22,23. Correspondingly, the transcript levels of genes downstream of RIN, such as ripening-related, ethylene-related and cell-wall modification genes, were also examined. The results showed that the ripening-related genes CORLORLESS NON-RIPENING (CNR), TDR4, APETALA2 (AP2a), and NON-RIPENING (NOR) were decreased to different degrees (Fig. 3C). Furthermore, the expression levels of two ethylene biosynthetic genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase 2 (ACS2) and ACS4, were also reduced in overexpression transgenic tomatoes (Fig. 3C). Certain cell-wall modification genes showed a slight decrease, including Polygalacturonase (PG), Endo-(1,4)- β-mannanase 4 (MAN4), and β-Galactosidase 4 (TBG4), which are thought to be related to the softening and shelf life of fruit (Fig. 3C).
Finally, qRT-PCRs were performed to detect the above genes transcription levels in SlMSI1 suppression transgenic lines. In accordance with our hypothesis, the transcript levels of RIN and its regulons (ripening-related, ethylene-related and cell-wall modification genes) showed little change in the suppression lines (Fig. 3D). Taken together, these results suggest that SlMSI1 inhibits fruit ripening by repressing the expression of RIN and its downstream genes in tomato.
SlMSI1 suppresses a large set of fruit-ripening genes, including, but not limited to, RIN and its regulon
SlMSI1 overexpression transgenic fruits exhibited a more distinct non-ripening phenotype than the rin fruits, suggesting that SlMSI1 likely inhibits fruit ripening by regulating other genes in addition to RIN and its regulon. To test this hypothesis, RNA-seq assays were conducted using SlMSI1 overexpression transgenic line L29 and control fruits at the mature green stage. The results showed that 5269 genes were up-regulated and 1864 genes were down-regulated in overexpression line L29 by more than two-fold compared to the control (Fig. 4A). The down-regulated genes included RIN and most genes in its regulon (Fig. 4B, Supplementary Table S1). Additionally, the expression levels of other ripening genes, such as E4 and E8, were repressed by SlMSI1 (Fig. 4C), which explains why the non-ripening phenotype of SlMSI1 transgenic fruits is more pronounced than that of the rin mutant. Therefore, these results indicate that SlMSI1 controls fruit ripening genes that include, but are not limited to, RIN and its regulon during fruit ripening. In addition, SlMSI1 regulated the expression levels of homeotic genes, i.e., AP3 and PI, and its transgenic lines exhibited abnormalities in the floral and other reproductive phenotypes (Fig. 4C).
Genetic manipulation of SlMSI1 and RIN prolongs fruit shelf life
To examine the shelf life, breaker fruits of the control, rin mutant and 3 overexpression lines (L1, L2 and L29) were harvested and placed at room temperature. The control fruit turned completely red 40 days after harvest (DAH) and began to shrink, whereas the rin fruits exhibited a pale yellow color and started to shrink at 50 DAH. In contrast, the fruits of the 3 overexpression lines remained green at 50 DAH (Fig. 5A). In addition, dehydration assays were performed using the fruits at 30, 40 and 50 days after the breaker stage (B + 30 d, B + 40 d and B + 50 d, respectively). The results showed that there was less water loss in the 3 overexpression lines than in the control and even less water loss in the rin fruits at all 3 tested stages (Fig. 5B).
Furthermore, RIN (Solyc05g012020.2.1) was genetically transformed into SlMSI1 transgenic line L29 to produce three recovery lines, R1, R2 and R3, with a L29 background to investigate expression rescue (Fig. 5C). The three lines produced many more RIN transcripts than L29 but fewer than the control. Additionally, the expression levels of the genes in the RIN regulon, such as PG, ACS2, ACS4, MAN4 and TBG4, were partially recovered in the 3 recovery lines (Fig. 5D). However, the transcript levels of the other ripening genes, such as CNR, NOR, TDR4, and AP2a, did not increase in the three recovery lines compared with L29. As a result, the fruits of the R1, R2 and R3 lines ripened more quickly than did the L29 fruits. Compared with the control fruits, the fruits of the three recovery lines exhibited a noticeably prolonged shelf life, as indicated by their non-shrunken appearance and reduced dehydration (Fig. 5E,F).
Discussion
MSI1 protein contains WD-40 repeat domains and is a member of the evolutionarily conserved PcG complexes11. In Arabidopsis, MSI1 has been found to interact with other PcG proteins to form diverse PRC2 complexes, i.e., the VRN complex that accelerates flowering in response to prolonged exposure to cold12,13; the EMF complex that controls vegetative development and the transition to flowering9,14; and the FIS complex that specially functions in female gametophyte and endosperm development10,11. In addition to the PRC2 complex, MSI1 is an essential component of the Arabidopsis chromatin assembly factor (CAF-1) complex, as are the proteins FASCIATA 1 (FAS1) and FASCIATA 2 (FAS2)31. The CAF-1 complex is conserved in yeast, Drosophila and mammals and is essential for the deposition of the heterodimer H3-H4 at the replication fork32,33. In tomato, LeMSI1/SlMSI1 has been found to interact with distinct proteins during fruit development28, suggesting that SlMSI1 might be involved in regulating fruit development. Here, we found that SlMSI1 overexpressing lines displayed the abnormal flowers with larger sepals and indeterminacy inflorescences (Fig. 2B,C). MACROCALYX (MC) affects inflorescence determinacy and sepal development. qRT-PCR data showed that MC was decreased in all three ovexpression transgenic lines and recover lines, but changed a little in suppression lines (Supplementary Fig. 2). The results suggested that abnormal flower development might be induced by MC. Additionally, overexpressed SlMSI1 resulted in non-ripening fruits with a long shelf life. In Arabidopsis, MSI1 has been shown to function in flower and seed development34,35. We found that SlMSI1 overexpression and suppression transgenic tomatoes were affected with respect to both flower development and fruit ripening (Fig. 2E), which is indicative of a novel role for SlMSI1 in fleshy fruit species.
Fruit ripening is a complex process accompanied by numerous developmental and metabolic changes36,37. In this study, SlMSI1 inhibited fruit ripening by negatively regulating ripening-related genes (RIN, CNR and TDR4), ethylene synthesis genes (ACS2 and ACS4) and cell-wall modified genes (PG and MAN4), all of which have been reported to be regulated by RIN 19,22. Previous studies have noted the complicated regulatory network of RIN and its target genes during fruit ripening; however, the potential role and mechanism of how these regulators act upstream of RIN to control fruit ripening remain unknown. Here, it was found that RIN and its target genes were markedly down-regulated in SlMSI1 overexpression transgenic tomatoes. Furthermore SlMSI1 protein and transcript levels did not change in the rin mutant, indicating that SlMSI1 acts upstream of RIN and regulates fruit ripening as a negative modulator. Therefore, our findings provide a new component of the emerging knowledge of mechanisms regulating fleshy fruit ripening.
Recently, it has been reported that DNA methylation in promoters of typical ripening genes are gradually decreased during ripening16,17, providing insight into the role of epigenetic DNA modifications on fruit ripening and their potential utilization in breeding programs for improving fruit shelf life. Furthermore, another PcG protein, SlEZ2, regulates vegetative and reproductive development of tomato fruits and has a strong influence on the global level of H3K27me327. Notably, SlMSI1 belongs to a subfamily of WD-40 repeat proteins, which are evolutionarily conserved developmental suppressors that act via DNA methylation and histone methylation1,3. In this study, it was found that the protein and transcript levels of SlMSI1 were clearly decreased at the onset of ripening during fruit development, whereas the expression levels of RIN and its target genes changed in an inverse manner. It appears that the SlMSI1-associated PRC2 complex and the related DNA methylation modification likely contributed to the inhibition of the RIN regulon and fruit ripening. Recently, numerous transcription factors associated with H3K27me3 markers have been identified, such as certain MADS-box factors38.
One major goal of fruit improvement is to reduce post-harvest wastage. A long shelf life is a desired trait for both breeders and purchasers. With an increasing understanding of the molecular mechanisms by which plants regulate fruit ripening and the advent of genetic engineering technologies, researchers have focused on finding new strategies to address problems related to fruit shelf life. Commercial breeding is currently focused on genetic polymorphisms. In this study, it was found that the PcG protein SlMSI1 negatively regulates RIN and other ripening genes, and genetic manipulation of SlMSI1 and RIN successfully prolonged the fruit shelf life, demonstrating that SlMSI1 is a novel regulator suitable for use in genetic engineering modification of fruit shelf life.
Materials and Methods
Plant materials and growth conditions
Tomato cultivars (Solanum lycopersicum cv. Ailsa Craig) and rin mutant were grown in greenhouses under natural conditions (16 h supplemental lighting at 25 °C and 8 h at 16 °C). Fruit ripening stages of tomato were divided according to days after anthesis (dpa) and color changes39. Flowers were tagged at anthesis. In wild type fruits, mature green (MG) were defined as 45 dpa and were characterized as fully expanded unripe fruit with mature seeds. Breaker (B) fruits were defined as 55 dpa and the color change from green to yellow. Fruits at 65 DAF were fully ripe and can vary substantially among cultivars.
RNA extract, qRT-PCR and RNA-seq analysis
Harvested tomato tissues were immediately frozen in liquid nitrogen and stored at −80 °C. Total RNAs were isolated using Trizol (Invitrogen) according to the manufacturer’s instructions and used for PCR and RNA-seq analysis. For qRT-PCR analysis, the first-strand cDNA was synthesized using an M-MLV system. The qRT-PCR was performed using specific primers (Supplementary Table S2) as described by Liu et al.40. Three technical replicates were performed for each sample every time and three biological repeats were performed. The relative quantitative values were calculated using the 2−△△Ct method41. The specificity of the amplification was determined by performing a dissociation curve analysis.
For RNA-seq, total RNA samples were prepared from the fruits at the breaker stage (55 dpa) using Trizol (Invitrogen) and purified with the RNeasy Plus Kit (Qiagen). Then, the RNA was analyzed using a high-throughput parallel sequencing using an Illumina genome analyzer II according to the manufacturer’s instructions. The false discovery rate (FDR) was set at 1% to determine the threshold of the P-value in multiple tests and analyses by manipulating the FDR value. P < 0.001 and the absolute value of log2 ratio >1 were used as the threshold to judge the significance of the gene expression difference according to Audic and Claverie42.
Protein extraction and Western blot analysis
SlMSI1 protein level was detected using tomato leaves and breaker stage fruits, respectively. All proteins were extracted as described by Li et al.43. The protein concentration was determined using the Bradford reagent (Sigma-Aldrich) with bovinese rumalbum as a standard.
Anti-SlMSI1 polyclonal antibody was commissioned from GenScript Company. Protein extracts (30 μL) were lysed in gel-loading buffer containing 50 mM Tris-HCl (pH 6.8), 100 mM dithiothreitol (DTT), 2% sodium dodecyl sulfate (SDS), 0.1% bromophenol blue, and 10% glycerol. Fifty micrograms of protein was resolved using SDS-polyacrylamide gel electrophoresis (PAGE) and electrically blotted onto a nylon filter (Roche). The filters were blocked with phosphate-buffered saline (PBS) containing 15% nonfat milks and incubated with specific antibody of anti-SlMSI1 (1:5000), polyclonal antibody ACTIN (1:5000, Abcam) was used as control, respectively. After washing with PBS containing 0.5% Tween 20, the bound primary antibody was detected using anti-mouse IgG or anti-rabbit IgG (1:10000, Abcam). After washing, the anti-specific antibodies or anti-control antibodies bound proteins were visualized using Immobilon Western chemiluminescent HRP substrate kit (Millipore).
Generation of transgenic tomato plants
Overexpression constructs were made by cloning the SlMSI1 (Solyc01g104510.2.1) cDNA using the BamHI restriction enzyme into the pBIN-GFP vector. The SlMSI1 suppression construct was generated using antisense sequence of its 3′-UTR. All constructs were sequence confirmed and transformed into Agrobacterium tumefaciens (strain LBA4404) via electroporation. Tomato transformation was performed as described by Ellul et al.44. The transgenic plants were verified with PCR using two sets of primers. 35S and gene-specific reverse primers were used to amplify the region encompassing the end of the 35S promoter and transgene, while NPT2F and NPT2R were used to amplify the kanamycin resistance gene.
RIN was genetically transformed into SlMSI1 transgenic line L29. The recovery constructs were made by inserting the RIN cDNA (Solyc05g012020.2.1) using Xcm1 restriction enzyme into the pCXSN vector45. The resultant constructs were genetically transformed into SlMSI1 overexpression transgenic line L29 with an Agrobacterium method. The transgenic plants were chosen by both kanamycin- and hygromycin- resistance. Gene-specific primers were used to amplify the RIN gene. The specific primers are listed in Supplementary Table S2. Homozygous transgenic lines were used for investigation.
Dehydration assay
Dehydration analysis was performed using 10 fruits that were harvested at the breaker stage. The fruits were kept at room temperature for 30 days, 40 days and 50 days. The fresh weights were recorded every 10 days. The fresh weight of the fruit at the breaker stage was recorded as the starting point. The water loss was calculated as the percentage difference in weight between the starting weight and each subsequent measurement.
Ethylene measurement
Fruits of breaker, 1 day after breaker (B + 1), B + 3, 5, 7, 9, 11, 13, 15, and 17 were harvested and placed in open 500 ml jars for 2 h to minimize the effect of wound ethylene caused by picking. Jars were then sealed and incubated at room temperate for 24 h, 1 ml of headspace gas was injected into a Hewlett-Packard 5890 series gas chromatograph equipped with a flame ionization detector (FID). Samples were compared with reagent grade ethylene standards of known concentration and normalized for fruit weight46.
Firmness measurement
Firmness measurements were made by fruit firmness tester (GY-2, China). The maximum force recorded at 10 mm of compression was used as an estimation of the fruit firmness from the averaged value of at least three tested fruits with a minimum of three compressions per fruit.
Statistical analysis
The mean values of qRT-PCR and dehydration measurement were taken from the measurements of three biological replicates, and SE was calculated. Statistical analysis of the data was tested with ANOVA and significant difference at the 5% level using the SPSS 16.0 software package.
Additional Information
How to cite this article: Liu, D.-D. et al. Polycomb-group protein SlMSI1 represses the expression of fruit-ripening genes to prolong shelf life in tomato. Sci. Rep. 6, 31806; doi: 10.1038/srep31806 (2016).
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Acknowledgements
We thank BGI technology (China) for technical assistance in RNA-seq, mass spectrometry and data analysis. This work was supported by grants from the Ministry of Science and Technology of China (2013CB945103), NSFC (31325024, 31272142) and the Ministry of Education of China (IRT15R42).
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Y.-J.H. and D.-D.L. designed research; D.-D.L., M.-J.F. and L.-J.Z.performed research; Q.-L.D., X.-H.A. and C.-X.Y. contributed new eagents tools; D.-D.L., L.-J.Z. and Q.-L.D. analyzed data; Y.-J.H. and D.-D.L wrote the paper.
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Liu, DD., Zhou, LJ., Fang, MJ. et al. Polycomb-group protein SlMSI1 represses the expression of fruit-ripening genes to prolong shelf life in tomato. Sci Rep 6, 31806 (2016). https://doi.org/10.1038/srep31806
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DOI: https://doi.org/10.1038/srep31806
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