Abstract
Biotreatment of hexavalent chromium has attracted widespread interest due to its cost effective and environmental friendliness. However, the difficult separation of biomass from aqueous solution and the slow hexavalent chromium bioreduction rate are bottlenecks for biotechnology application. In this approach, a core-shell structured functional polymer coated magnetic nanocomposite was prepared for enriching the hexavalent chromium. Then the nanocomposite was connected to the bacteria via amines on bacterial (Bacillus subtilis ATCC-6633) surface. Under optimal conditions, a series of experiments were launched to degrade hexavalent chromium from the aqueous solution using the as-prepared bionanocomposite. Results showed that B. subtilis@Fe3O4@mSiO2@MANHE (BFSM) can degrade hexavalent chromium from the water more effectively (a respectable degradation efficiency of about 94%) when compared with pristine B. subtilis and Fe3O4@mSiO2@MANHE (FSM). Moreover, the BFSM could be separated from the wastewater by magnetic separation technology conveniently due to the Fe3O4 core of FSM. These results indicate that the application of BFSM is a promising strategy for effective treating wastewater containing hexavalent chromium.
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Discover the latest articles, news and stories from top researchers in related subjects.Introduction
Under natural conditions, the atmosphere, soil and water contains trace amounts of chromium compound. Hexavalent chromium mainly exists in forms of Cr2O72− and CrO42− which is highly mobile1, water soluble and toxic to all living organisms2. Because of high solubility, hexavalent chromium goes into the living cells easily and produces reactive oxygen species (ROS), resulting in serious oxidative injuries to cell constituents3. The main effects of hexavalent chromium for humans are dermatitis and aggressive reaction in lungs and nasal septum4,5. The maximum total chromium concentration in water body is limited to 0.1 mg/L according to EPA drinking water standards6,7,8,9. However, the concentration of hexavalent chromium is over 1000 times in the ordinary wastewater9,10,11,12. Unfortunately, chromium is widely used in numerous industrial processes, including leather tanning, pigment production, electroplating and ore refining13,14,15,16. In this context, if the industrial containing chromium wastewater could not be effectively addressed, it may lead to the contamination of natural water sources and ultimately threatening human health17,18,19,20,21,22.
Because of this, far-ranging conventional methodologies have been used for water purification, including filtration and coagulation/sedimentation/flocculation, liquid extraction, chemical oxidation, membrane processes and so on23,24,25,26. However, these methodologies have been proved to be inefficient and uneconomic for the treatment of hexavalent chromium27,28. To overcome these disadvantages, a great deal of attentions have been concentrated on microbial remediation strategy for hexavalent chromium contamination through sorption, accumulation and reorganization1,29, which is considered as low-cost and eco-friendliness comparing with chemical methods30,31. Up to date, multifarious bacteria have the ability of reducing hexavalent chromium to less toxic Cr(III) under aerobic or anaerobic conditions32,33,34,35,36. For example, Bacillus sp., Ochrobactrum sp., Enterobacter sp., Pseudomonas sp., Pannonibacter sp., Arthrobacter sp., Acinetobacter sp. and Exiguobacterium sp. are such functional bacteria37,38. Nevertheless, biological treatment also has its own drawbacks, for example, it will take several days or even weeks to acheive the complete reduction of hexavalent chromium (especially at low concentration) under optimal conditions1,39. Moreover, the difficult separation of bacteria from the treated wastewater also limits its application39. Therefore, it is necessary to develop new biotechnologies that can effectively achieve hexavalent chromium reduction and rapidly separate bacteria from the wastewater after the treatment30. Recently, magnetic nanocomposite was exploited for heavy metal ions reduction due to its high magnetic separation efficiency39,40,41. Moreover, silica was reported as an ideal protection layer for Fe3O4 NPs not only due to its high chemical stability and great biocompatibility but also its highly reactive surface42,43,44. In addition to these, polymers including polyacrylamide, polyaniline (PANI) and polyethylenimine had been taken increasing notice for heavy metal removal because of their high removal efficiency45,46,47. For instance, PANI, as a kind of conductive polymer, had been paid intensive attention for potential environmental applications because of its large surface area and abundant active sites48. Nowadays, many kinds of polymers had been coated on the surface of the easily separated materials including fibers49,50, sawdust, Fe3O4 nanoparticles, which showed excellent pollutants removal performance.
In this paper, a core-shell structured magnetic nanocomposite for efficient enrichment of chromium ions from water was first fabricated. Fe3O4 was selected as the core of nanomaterials due to its good magnetic separation efficiency. Then the Fe3O4 was coated with mesoporous silica (mSiO2), which have been used as promising adsorbents for the water remediation and provided advantages such as large surface area, high surface reactivity and regular mesoporous structures. In addition, the efficacy of mesoporous silica in the adsorption process could be highly improved via surface functionalization with particular groups for adsorption of specific substances. Based on this assumption, the surface of mesoporous silica was modified with amine groups and 4, 4-azo-bis(4-Cyanopentanoicchloride) (ABCPA, Fig. 1), an active radical initiator was grafted via these amine groups51. Functional polymer consisting 4-vinyl pyridine and N-(methacryloyloxy)-succinimide could encapsulate Fe3O4@mSiO2 via polymerization. Then, the nanocomposite was attached on the surface of bacteria through the coupling reaction between the N-(methacryloyloxy)-succinimide group of the nanocomposite and the amine group of the bacteria cells. Finally, magnetic bacteria systems were established to achieve rapid and effective degradation of hexavalent chromium and magnetic separation of bacteria.
Results and Discussion
Cr(VI) reduction by planktonic cells
In the present study, we investigated the hexavalent chromium reduction by planktonic cells of B. subtilis in a glucose solution. Figure 2a shows that 40 mg/L of hexavalent chromium was decreased to approximately zero by planktonic cells of B. subtilis within 96 h, while the OD600 of B. subtilis can be seen an obvious increasement which corresponding with the quantity of the bacteria cells. The result showed that bacteria could subsist very well in the presence of hexavalent chromium. The UV-vis spectrum of Cr-diphenylcarbazide complex was shown in Fig. 2b. It could be clearly seen that the adsorption value changed a lot and even decrease to zero after 96 h. This result strongly demonstrates the biodegradation of hexavalent chromium, which further confirm the biodegrade ability of B. subtilis.
To further investigate the degradation process of hexavalent chromium, the total chromium concentration of 10 mg/L, 20 mg/L, 30 mg/L and 40 mg/L after treated at different times were studied by atomic absorption spectrometry. As shown in Fig. 3, the total chromium concentration of 10 mg/L, 20 mg/L, 30 mg/L and 40 mg/L had no changes in the process of degradation even after 120 h. The above results indicated that the planktonic cells of B. subtilis only degrade hexavalent chromium without adsorption chromium ions.
In order to illustrate the morphologic changes and detail distribution of degraded hexavalent chromium on planktonic cells, SEM was performed and the results were shown in Fig. 4. As can be seen in Fig. 4a, the long–rod shaped B. subtilis have a smooth surface and the planktonic cells were plumb before the biodegradation of hexavalent chromium. After treated with 40 mg/L hexavalent chromium for 120 h, the surface of bacteria became rough and lean (Fig. 4b). EDS spectra demonstrated that a small amount of chromium was accumulated on the bacterial surfaces (Fig. 4b). This result further confirms that the planktonic cells of B. subtilis mainly degrade hexavalent chromium instead of adsorption.
Characterization of nanoparticles
FT-IR spectra of Fe3O4@mSiO2-NH2, Fe3O4@mSiO2–ABCPA (4,4-Azobis(4-cyanovaleric acid)), FSM nanocomposites are displayed in Fig. 5. As shown in the spectrum of Fe3O4@mSiO2-NH2, the peak observed at 580 cm−1 was characteristic of the Fe-O vibration. The peaks at 1076 and 3432 cm−1 were from the stretching vibration of Si-O and Si-OH bonds, separately. After grafting of free-radical initiator on the Fe3O4@mSiO2 magnetic nanocomposites, two new peaks at 1387 cm−1 and 1632 cm−1 corresponding to C-H symmetric and asymmetric bending vibrations of methyl groups in ABCPA were observed. The observation of the characteristic absorption peak at 2280 cm−1 representing the C≡N stretching vibrations of ABCPA also demonstrates successfully grafting of free-radical initiator to Fe3O4@mSiO2 nanocomposites. On the spectrum of FSM, the absorption peaks at 2918 cm−1 and 1596 cm−1 are identified as C-H asymmetric stretching vibration and C=O stretching vibration of the grafted MANHE chains.
Fe3O4@SiO2, FSM nanocomposites and MANHE were also characterized by TGA. As shown in Fig. 6, Fe3O4@SiO2 have about 20 wt.% weight loss in the range between 100 and 700 °C, which was corresponding to the loss of the functional groups such as OH groups on the surface of Fe3O4@SiO2. MANHE polymer showed about 82.5% weight loss of functional groups and carbon chain skeleton in the range between 100 and 700 °C. However, FSM nanocomposites showed about 68 wt.% weight loss after polymerization of 4-vinyl pyridine and N-(methacryloyloxy)-succinimide. All these results confirmed the successful polymer modification which was in good agreement with the FT-IR (Fig. 5).
FSM, B. subtilis and BFSM were prepared under optimal conditions and then characterized by XRD. As can be seen in Fig. 7a, the characteristics of the crystal plane diffraction peaks (220, 311, 400) of Fe3O4 appeared at 35.2°, 41.7°, 50.6°, respectively. Meanwhile, the peak at 16 ∼ 36° indexed as (001) could be attribute to characteristic diffraction peaks of the amorphous SiO2. The results indicated that the crystal form of the nanoparticles did not change after the coating of MANHE. The XRD spectra of B. subtilis was shown in Fig. 7b, there are no obvious characteristic peaks. And the XRD spectra of BFSM were shown in Fig. 6c, an obvious peak at 35° was corresponding to the magnetic core. This result strongly indicated that the FSM have been successfully modified to the surface of bacteria. Moreover, the results of above XRD further support the results of FT-IR.
The structure and morphological features of magnetic nanoparticles were further examined by TEM (Fig. 8). The images of TEM obviously indicate that monodispersed microspheres with narrow size distribution were obtained for all the samples. As shown in Fig. 8a, the Fe3O4 synthesized by hydrothermal method had a uniform diameter at about 30 nm with excellent dispersion. In addition, from the images of Fe3O4@mSiO2 and FSM nanoparticles (Fig. 8b,c), the core-shell structure could be clearly distinguished owing to the different electron penetrability between Fe3O4, SiO2 and MANHE. As shown in Fig. 8b, Fe3O4@mSiO2 was spherical and the thickness of the mesoporous silica layer was about 20 nm. As shown in the TEM image of FSM (Fig. 8c), the magnetic nanocomposites modified by polymer with large amounts of nitrogen remained spherial. The thickness of MANHE shell showed gray color was about 30 nm (Fig. 8c). This result indicated that the functional polymer was well coated on the surface of Fe3O4@mSiO2.
As shown in Fig. 8d, the functional nanocomposites were modified on the surface of bacteria via amino groups, which were obviously indicated in TEM images (Fig. 8d), because the bacterial surface became rough and lean. As the inset of Fig. 8d shown, the magnetic separation of BFSM was examined to analyze the magnetism qualitatively. BFSM could be separated easily and completely about 2 minutes under the applied external magnetic field. In addition, BFSM could easily and steadily disperse in water by shaking. This result indicated that BFSM had superior strong magnetism and eminent dispersibility, which were helpful in practical applications.
The degradation of hexavalent chromium
To study the ability of bionanocomposite to degrade hexavalent chromium, B. subtilis, FSM and BFSM were prepared to degrade 40 mg/L solution of hexavalent chromium with the same time under the optimal conditions respectively. The results were shown in Fig. 9a, the planktonic cells of B. subtilis degrade hexavalent chromium with the fastest rate in solution within 0 h to 8 h. Then, the concentration of hexavalent chromium would achieve equilibrium after 70 h. The removal efficiency of hexavalent chromium would reach 83.4% after 120 h. On the other hand, the concentration of hexavalent chromium would reach equilibrium after 4 h when treated by FSM. Meanwhile, only 50% of hexavalent chromium would be degraded from the 40 mg/L hexavalent chromium solution after 120 h. When treated by BFSM, it only spend 2 h for 50% removal of hexavalent chromium and the equilibrium achieved after 96 h. it was noted that 94% of hexavalent chromium was removed from the 40 mg/L hexavaient chromium solution after 120 h. The UV-vis spectrum of 40 mg/L of hexavaient chromium treated by B. subtilis, FSM and BFSM were shown in Fig. 9 respectively (panels b, c and d). It is observed that the BFSM is more efficient in reduction of hexavaient chromium than the B. subtilis (Fig. 9b) and FSM (Fig. 9c). Because the UV-vis spectrum of hexavalent Cr-diphenylcarbazide complex was shown in Fig. 9d at 365 nm. It could be clearly seen that the adsorption value decrease more quickly than B. subtilis (Fig. 9b) and FSM (Fig. 9c). The Fig. 9b showed that there were no longer absorption band of hexavalent chromium centered at 365 nm for 120 h. The result was also in agreement with the Fig. 2b. So we can consider that the concentration of hexavalent chromium is close to zero according to results shown in Fig. 9b. As seen in Fig. 9c, the concentration of hexavalent chromium had no change after 24 h and achieved absorption equilibrium. However, no absorption band of hexavalent chromium centered at 365 nm was observed and the adsorption value even decrease to zero after 72 h. In this process of degradation, absorption and degradation of Cr(VI) would be combined into continuous process: (1) The BFSM surface of the absorptive polymer would efficiently bind Cr(VI) and then Cr(VI) absorbed on the surface of B. subtilis. (2) The degradation process would be promoted due to a higher local concentration of Cr(VI) on the surface of B. subtilis. Through the above comparison, the result strongly demonstrate the degradation of hexavalent chromium (Fig. 9), which further confirm the degrade ability of BFSM.
Conclusions
In this study, an advanced bionanocomposites was prepared for removing the hexavalent chromium from wastewater. A core-shell structured, magnetic nanocomposite modified by functional polymer was prepared for local enriching the low concentrations hexavalent chromium. Then the core-shell structured, magnetic nanocomposite was connected on the surface of bacteria via amines on bacterial (Bacillus subtilis ATCC-6633) surface. After the immobilization, the adsorption-biodegradation process could be illustrated with two stages. In the first stage, the adsorption process played a main role and the hexavalent chromium concentration could increase relatively around the bacteria in a short time. And then, the biodegradation started in the second stage and completed the whole process. BSFM spent nearly 72 h achieving 94% the degradation efficiency of hexavalent chromium. In the meantime, the BFSM could be separated from the wastewater via its magnetism. Therefore, this composited technique can be potentially applied in the treatment of low-concentrated Cr(VI)-containing wastewater.
Methods
Materials
Ethylene glycol (EG), anhydrous sodium acetate (NaAc), ammonia solution (NH3·H2O, 28 wt.%), iron nitrate (Fe(NO3)3·9H2O), cetyltrimethylammonium bromide (CTAB), tetraethylorthosilicate (TEOS) were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai); Potassium dichromate (K2Cr2O7) and Dlphenylcarbazide were purchased from Aldrich; 4,4-Azobis(4-cyanovaleric acid) (ABCPA, 97%) and N-Acryloxysuccinimide was purchased from Sigma; Tryptone and yeast extract were supplied with Suzhou Biogene Biotechnology Co., Ltd. B. subtilis ATCC-6633 was obtained from Fujian Institute of Microbiology, China. All reagents were used as received without further purification.
Synthesis of spherical Fe3O4
In this experiment, all the chemical agents were of analytical grade and were used without further purification. The spherical magnetic particles were prepared according to the literature with some modification42. As usually, 2.02 g of Fe(NO3)3·9H2O and 4.1 g of sodium acetate were dissolved in 50 mL of ethylene glycol (EG) with stirring for 30 min. The obtained solution was transferred to a Teflon-lined stainless-steel autoclave and heated at 180 °C for 6 h. Then the autoclave was naturally cooled to room temperature. The gained black magnetite particles were washed with ethanol for several times and dried in vacuum at 60 °C for 5 h.
Synthesis of Fe3O4@mSiO2
The core–shell structured Fe3O4@mSiO2 microspheres were prepared through a modified Stöber method. In a typical process, 0.10 g of obtained Fe3O4 particles were treated using 0.1 M HCl solution by ultrasonication for 20 min. Whereafter, the treated Fe3O4 particles were separated via centrifugation, washed with deionized water. At the same time, The Fe3O4 was dispersed in the mixture solution of 80 mL of ethanol, 20 mL of deionized water and 1.0 mL of concentrated ammonia aqueous solution (28 wt.%). Afterward, 0.3 g of cetyltrimethylammonium bromide (CTAB) was added dropwise to the solution. After this, 0.25 mL TEOS was added dropwise into the solution under vigorous stirring for 6 h. After reaction for 6 h, the product was collected by magnetic separation and tautologically washed with ethanol and deionized water. The above coating process was redone twice. The structure-directing agent (CTAB) was removed with ethanol and deionized water for three times. The obtained precipitate was separated and washed with deionized water. Subsequently, the product was dried in vacuum at 60 °C for 24 h. The manufactured microspheres what was called Fe3O4@mSiO2.
Synthesis of Fe3O4@mSiO2-NH2
200 mg (Fe3O4@mSiO2) of the nanoparticles obtained for 250 ml flask, the flask to add 150 ml acetonitrile, ultrasound 30 min, then add 3 ml KH550, mechanical agitation for the night.
Synthesis of Fe3O4@mSiO2@MANHE
The acid chloride derivative of ABCPA (Cl-ABCPA) was prepared by a reaction of ABCPA and PCl5. ABCPA (3.0 g) was dissolved in dichloromethane (25 mL) and cooled to 0 °C. PCl5 (24 g) in 25 mL of CH2Cl2 was added into the above solution and stirred overnight. After the reaction, the excess PCl5 was removed by filtration. The clear solution was added into 5-fold of hexane at 0 °C and 4,4-azo-bis(4-cyanopentanoicchloride) was obtained after filtration. Fe3O4mSiO2-NH2 nanoparticles (0.600 g) were added to 80 mL of dry dimethylformamide. After 0.5 h of ultrasonication, Fe3O4@mSiO2-NH2 (0.60 g) was dispersed in a mixture of 80 mL of CH2Cl2 and 2 mL of triethylamine and Cl-ABCPA (2.5 g) in 25 mL of dry CH2Cl2 was added to the dispersion. After stirring at 0 °C for 2 h, the dispersion was stirred at room temperature overnight. Fe3O4@mSiO2-ABCPA was obtained after filtration and washing with methanol and dichloromethane. Polymer on Fe3O4@mSiO2-ABCPA sheets were prepared by free-radical polymerization. In a Schlenk flask, Fe3O4@mSiO2-ABCPA (0.05 g), 4-vinylpyridine (4 mL) and N-Acryloxysuccinimide (0.7 g) monomer were dissolved in 9 mL of Cyclohexanone. After 0.5 h min sonication, the dispersion was stirred at 75 °C for 5 h. The resulting product was dissolved in acetone and centrifuged to remove the free polymer chains which were not anchored to the nanoparticles. The final product (FSM) was dried in vacuum at 50 °C.
Bacteria cultivation
B. subtilis ATCC-6633 was obtained from Fujian Institute of Microbiology, China. Previous study suggested that the maximum hexavalent chromium resistance of B. subtilis ATCC-6633 is 40 mg/L. Planktonic cells were grown at 30 °C with shaking (120 rpm) for 48 h in modified LuriaeBertani (LB) liquid medium (pH=7) supplemented with 5 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract and 5 g/L glucose.
Synthesis of BFSM
After strain B. subtilis ATCC-6633 was cultured for 48 h in 100 mL LB medium with shaking (120 rpm), the B. subtilis ATCC-6633 were harvested by centrifugation (5 min, 5500 g) and washed twice with PBS (sterile phosphate buffer solution). Then, the cell pellets were resuspended in PBS. Subsequently, 30 mg FSM was added into above system. Planktonic cells were cultivated at 30 °C with shaking (120 rpm) for 5 h. After that, BFSM were obtained by magnetic separation.
Chromium reduction and immobilization experiments
In order to prepare the chromium stock solution, K2Cr2O7 (AR) was dissolved in deionized-distilled water. In the experiments, the initial hexavalent chromium concentration was 40 mg/L. Cr-bacteria interaction experiments were conducted in two steps as follows: 1) planktonic cells were cultured in LB medium for 48 h and then harvested by centrifugation at 5500 g for 5 min; 2) bacteria were transferred into 100 mL glucose solution (5 g/L, pH = 7) with a final concentration of 0.01 g/mL and bacteria-Cr interaction experiments were conducted by adding chromium stock solution to the designed initial concentration at pH 7.0. At the same time, BFSM- Cr interaction experiments were conducted by adding chromium stock solution to the designed initial concentration under optimal conditions. At last, samples were periodically taken with a sterile needle and a syringe for the analysis of the Cr species.
Analytical methods
The UV-vis absorbance spectrum of hexavalent chromium and the concentrations of hexavalent chromium were obtained using a Shimadzu UV 3600 spectrophotometer equipped with an equipped with a MPC-3100 integrating sphere attachment. The total concentrations of Cr were obtained using atomic absorption spectrophotometer (PinAAcle 900T). The size of the bacteria and surface of bacteria were examined by scanning electron microscope (SEM; Hitachi S-4800). Transmission electron microscopy (TEM; Hitachi H600) was used to observe the transformation of nanoparticles in the synthesis process; Fourier transform infrared spectroscopy (FT-IR; Nicolet 4700) was employed to represent results after wrapping SiO2. The thermal properties of the composites were measured by thermogravimetric analysis (TGA). The samples were heated to 800 °C at a heating rate of 10 K/min under nitrogen atmosphere on a Netzsch TG209 instrument. X-ray diffraction (XRD) (X’Pert-Pro MPD) was taken to analyze the crystal phase.
Additional Information
How to cite this article: Zhong, Y. et al. Fabrication of Unique Magnetic Bionanocomposite for Highly Efficient Removal of Hexavalent Chromium from Water. Sci. Rep. 6, 31090; doi: 10.1038/srep31090 (2016).
References
Pan, X. H. et al. Investigation of Cr(VI) reduction and Cr(III) immobilization mechanism by planktonic cells and biofilms of Bacillus subtilis ATCC-6633. Water. Res. 55, 21–29 (2014).
Zhang, H. K., Lu, H., Wang, J., Zhou, J. T. & Sui, M. Cr(VI) Reduction and Cr(III) Immobilization by Acinetobacter sp. HK 1 with the Assistance of a Novel Quinone/Graphene Oxide Composite. Environ. Sci. Technol. 48, 12876–12885 (2014).
Guria, M. K., Guha, A. K. & Bhattacharyya, M. A green chemical approach for biotransformation of Cr(VI) to Cr(III), utilizing Fusarium sp. MMT1 and consequent structural alteration of cell morphology. J. Environ Chem Eng. 2, 424–433 (2014).
Mangaiyarkarasi, M., Vincent, S., Janarthanan, S., Rao, T. S. & Tata, B. Bioreduction of Cr(VI) by alkaliphilic Bacillus subtilis and interaction of the membrane groups. Saudi J. Biol. Sci. 18, 157–167 (2011).
Nancharaiah, Y. V., Dodge, C., Venugopalan, V. P., Narasimhan, S. V. & Francis, A. J. Immobilization of Cr(VI) and its reduction to Cr(III) phosphate by granular biofilms comprising a mixture of microbes. Appl. Environ. Microbiol. 76, 2433–2438 (2010).
Richard, F. C. & Bourg, A. Aqueous geochemistry of chromium: a review. Water Res. 25, 807–816 (1991).
Gupta, A. & Balomajumder, C. Simultaneous removal of Cr(VI) and phenol from binary solution using Bacillus sp. immobilized onto tea waste biomass. J. Water Process Eng. 6, 1–10 (2015).
Vlyssides, A. G. & Israilides, C. J. Detoxification of tannery waste liquors with anelectrolysis system. J. Environ. Pollut. 97, 147–152 (1997).
Banerjee, A. & Ghoshal, A. K. Phenol degradation performance by isolated Bacilluscereus immobilized in alginate. Int. J. Biodeter. Biodegr. 65, 1052–1060 (2011).
Wang, P. & Lo, I. M. C. Synthesis of mesoporous magnetic g-Fe2O3 and its application to Cr(VI) removal from contaminated water. Water Res. 43, 3727–3734 (2009).
Hu, J., Lo, L. M. C. & Chen, G. Comparative study of various magnetic nanoparticles for Cr(VI) removal. Sep. Purif. Technol. 56, 249–256 (2007).
Environmental Protection Agency Environmental Pollution Control Alter- natives, EPA/625/5-90/025, EPA/625/4-89/023, Cincinnati (1990).
Zhao, Y. G., Shen, H. Y., Pan, S. D., Hu, M. Q. & Xia, Q. H. Preparation and characterization of amino-functionalized nano-Fe3O4 magnetic polymer adsorbents for removal of chromium (VI) ions. J. Mater. Sci. 45, 5291–5301 (2010).
Boening, D. W. Ecological effects, transport and fate of mercury: A general review. Chemosphere. 40, 1335–1351 (2000).
Bose-O’Reilly, S., McCarty, K. M., Steckling, N. & Lettmeier, B. Mercury exposure and children’s health. Curr. Probl. Pediatr. Adolesc. Health Care. 40, 186–215 (2010).
World Health Organization Guidelines for Drinking-water Quality, 3rd ed., Incorporating The First and Second Addenda, Vol 1, WHO Press: Geneva, 1–668 (2008).
Lu, X., Huangfu, X. L. & Ma, J. Removal of trace mercury(II) from aqueous solution by in situ formed Mn–Fe (hydr)oxides. J. Hazard. Mater. 280, 71–78 (2014).
Lv, X. S., Xu, J., Jiang, G. & Xu, X. H. Removal of chromium (VI) from wastewater by nanoscale zero-valent iron particles supported on multiwalled carbon nanotubes. Chemosphere. 85, 1204–1209 (2011).
Wu, L. M. et al. Micro-electrolysis of Cr (VI) in the nanoscale zero-valent iron loaded activated carbon. J. Hazard Mater. 254, 277–283 (2013).
Quievryn, G., Messer, J. & Zhitkovich, A. Carcinogenic chromium (VI) induces cross-linking of vitamin C to DNA in vitro and in human lung A549 cells. Biochemistry. 41, 3156–3167 (2002).
Thomas, D. H., Rohrer, J. S., Jackson, P. E., Pak, T. & Scott, J. N. Determination of hexavalent chromium at the level of the California Public Health Goal by ion chromatography. J. Chromatogr. A. 956, 255–259 (2002).
Pouran, H. M., Fotovat, A., Haghnia, G., Halajnia, A. & Chamsaz, M. A case study: chromium concentration and its species in a calcareous soil affected by leather industries effluents. World Appl. Sci. J. 5, 484–489 (2008).
Pazos, M., Branco, M., Neves, I. C., Sanroman, M. A. & Tavares, T. Removal of Cr(VI) from aqueous solutions by a bacterial biofilm supported on zeolite: optimisation of the operational conditions and scale-up of the bioreactor. Chem. Eng. Technol. 33, 2008–2014 (2010).
Davis, T. A., Volesky, B. & Mucci, A. A review of the biochemistry of heavy metal biosorption by brown algae. Water. Res. 37, 4311–4330 (2003).
Rawlings, D. E., Dew, D. & Du Plessis, C. Biomineralization of metal-containing ores and concentrates. Trends Biotechnol. 21, 38–44 (2003).
Wang, J. L. & Chen, C. Biosorbents for heavy metals removal and their future. Biotechnol. Adv. 27, 195–226 (2009).
Shams Khorramabadi, G., Darvishi Cheshmeh Soltani, R., Rezaee, A., Khataee, A. R. & Jonidi Jafari, A. Utilisation of immobilized activated sludge for the biosorption of chromium (VI). Can. J. Chem. Eng. 90, 1539 (2012).
Shirzad Siboni, M., Samadi, M. T., Yang, J. K. & Lee, S. M. Photocatalytic reduction of Cr(VI) and Ni(II) in aqueous solution by synthesized nanoparticle ZnO under ultraviolet light irradiation: A kinetic study. Environ. Technol. 32, 1573 (2011).
Barrera-Díaz, C. E., Lugo-Lugo, V. & Bilyeu, B. A review of chemical, electrochemical and biological methods for aqueous Cr(VI) reduction. J. Hazard. Mater. 223, 1–12 (2012).
Alvarez, L. H., Perez-Cruz, M. A., Rangel-Mendez, J. R. & Cervantes, F. J. Immobilized redox mediator on metal-oxides nanoparticles and its catalytic effect in a reductive decolorization process. J. Hazard. Mater. 184, 268–272 (2010).
Cervantes, F. J., Garcia-Espinosa, A., Moreno-Reynosa, M. A. & Rangel-Mendez, J. R. Immobilized redox mediators on anion exchange resins and their role on the reductive decolorization of azo dyes. Environ. Sci. Technol. 44, 1747–1753 (2010).
Bhattacharya, A. & Gupta, A. Evaluation of Acinetobacter sp. B9 for Cr (VI) resistance and detoxification with potential application in bioremediation of heavy-metals-rich industrial waste water. Environ. Sci. Poll. Res. 20, 6628–6637 (2013).
Cheng, G. J. & Li, X. H. Bioreduction of chromium(VI) by Bacillus sp. isolated from soils of iron mineral area. Eur. J. Soil Biol. 45, 483–487 (2009).
Das, S. et al. Investigation on mechanism of Cr (VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil. Chemosphere. 96, 112–121 (2014).
Xu, W. Y. et al. Characterization of Cr(VI) resistance and reduction by Pseudomonas aeruginosa. Trans. Nonferrous Met. Soc. China. 19, 1336–1341 (2014).
Zhu, W. J. et al. Anaerobic reduction of hexavalent chromium by bacterial cells of Achromobacter sp. strain Ch1. Microbiol. Res. 163, 616–623 (2008).
Kathiravan, M. N., Karthick, R. & Muthukumar, K. Ex situ bioremediation of Cr(VI) contaminated soil by Bacillus sp: batch and continuous studies. Chem. Eng. J. 169, 107–115 (2011).
Desai, C., Jain, K. & Madamwar, D. Evaluation of in vitro Cr(VI) reduction potential in cytosolic extracts of three indigenous Bacillus sp. isolated from Cr(VI) polluted industrial landfill. Bioresour. Technol. 99, 6059–6069 (2008).
Shao, M. et al. Preparation of double hydroxide core-shell microspheres for magnetic separation of proteins. J. Am. Chem. Soc. 134, 1071–1077 (2012).
Mu, Y., Ai, Z. H., Zhang, L. Z. & Song, F. H. Insight into Core–Shell Dependent Anoxic Cr(VI) Removal with Fe@Fe2O3 Nanowires: Indispensable Role of Surface Bound Fe(II). ACS Appl. Mater. Interfaces. 7, 1997–2005 (2015).
Zhu, S. B., An, Z. W., Chen, X. B., Chen, P. & Liu, Q. F. Approach to tune short-circuit current and open-circuit voltage of dye-sensitized solar cells: p-linker modification and photoanode selection. RSC Adv. 4, 38192–38198 (2014).
Yang, P. et al. A magnetic, luminescent and mesoporous core–shell structured composite material as drug carrier. Biomaterials. 30, 4786–4795 (2009).
Ying, Y. L. et al. Two-Dimensional Titanium Carbide for Efficiently Reductive Removal of Highly Toxic Chromium(VI) from Water. ACS Appl. Mater. Interfaces. 7, 1795–1803 (2015).
Chen, L. L., Zhao, D. L., Chen, S. H., Wang, X. B. & Chen, C. L. One-step fabrication of amino functionalized magnetic graphene oxidecomposite for uranium(VI) removal. J. Colloid. Interface Sci. 472, 99–107 (2016).
Lin, Y. C. & Wang, S. L. Chromium(VI) Reactions of Polysaccharide Biopolymers. Chem. Eng. J. 181–182, 479–485 (2012).
Wang, S. L. & Lee, J. F. Reaction Mechanism of Hexavalent Chromium with Cellulose. Chem. Eng. J. 174, 289–295 (2011).
Qiu, B. et al. Polyethylenimine Facilitated Ethyl Cellulose for Hexavalent Chromium Removal with a Wide pH Range. ACS Appl. Mater. Interfaces. 6, 19816–19824 (2014).
Olad, A. & Nabavi, R. Application of polyaniline for the reduction of toxic Cr(VI) in water. J. Hazard. Mater, 147, 845–851 (2007).
Qiu, B. et al. Polyaniline coating on carbon fiber fabrics for improved hexavalent chromium removal. RSC Adv, 4, 29855–29865 (2014).
Zhao, Y., Zhao, D. L., Chen, C. L. & Wang, X. K. Enhanced photo-reduction and removal of Cr(VI) on reduced grapheme oxide decorated with TiO2 nanoparticles. J. Colloid. Interface Sci. 405, 211–217 (2013).
Yang, Y. F. et al. Preparation of Reduced Graphene Oxide/Poly(acrylamide) Nanocomposite and Its Adsorption of Pb(II) and Methylene Blue. Langmuir. 29, 10727–10736 (2013).
Acknowledgements
We gratefully acknowledge the financial support provided by National Natural Science Foundation of China (21336005, 21301125 and 51573122), Natural Science Foundation of the Education Committee of Jiangsu Province (15KJB150026), Environmental Protection Research Foundation of Suzhou and Suzhou Nano-project (ZXG2013001, ZXG201420).
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J.-m.L. and D.C. conceived the project; Y.Z. designed and performed the experiments; X.Q., N.L., Q.X., H.L. and J.H. contributed to data analysis; Y.Z. prepared the manuscript. All the authors reviewed the manuscript.
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Zhong, Y., Qiu, X., Chen, D. et al. Fabrication of Unique Magnetic Bionanocomposite for Highly Efficient Removal of Hexavalent Chromium from Water. Sci Rep 6, 31090 (2016). https://doi.org/10.1038/srep31090
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DOI: https://doi.org/10.1038/srep31090
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