Abstract
Biological soil crusts can have strong effects on vascular plant communities which have been inferred from short-term germination and early establishment responses. However, biocrusts are often assumed to function as an “organizing principle” in communities because their effects can “cascade” to interactions among crust-associated plant species. We conducted surveys and experiments to explore these cascades and found that biocrusts were positively associated with large patches (> 10 m diameter) of a dominant shrub Artemisia tridentata. At the smaller scale of individual shrubs and the open matrices between shrubs, biocrusts were negatively associated with Artemisia. Juveniles of Artemisia were found only in biocrusts in intershrub spaces and never under shrubs or in soil without biocrusts. In two-year field experiments, biocrusts increased the growth of Festuca and the photosynthetic rates of Artemisia. Festuca planted under Artemisia were also at least twice as large as those planted in open sites without crusts or where Artemisia were removed. Thus, biocrusts can facilitate vascular plants over long time periods and can contribute to a “realized” cascade with nested negative and positive interactions for a range of species, but unusual among documented cascades in that it includes only autotrophs.
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Introduction
Biological soil crusts cover much of the soil surface in the arid and semi-arid regions of the world and have been described an “organizing principle”1,2,3. Soil crusts are highly variable and can be composed of nitrogen-fixing cyanobacteria and cyanolichens, lichens associated with green algae, free-living green algae and diatoms, fungi, mosses, and liverworts4. These diverse assemblages are of primary importance to vascular plant communities through their effects on soil water and fertility1,5,6. Biological soil crusts (biocrusts hereafter), can increase rainwater infiltration, improve soil moisture retention, increase soil organic matter, and prevent soil erosion1,3,5. Mechanistically, as biocrusts hydrate and desiccate, they can release large amounts of carbon and nitrogen into ecosystems7. Collectively, these effects can directly facilitate plants.
Biocrusts typically form a complex matrix with vascular plants at both small and large scales8,9. This soil/biocrust matrix forms a far more variable “playing field” for plant communities than soil without biocrusts because various plant species respond differently to one physical component or the other9,10. The effect of biocrusts corresponds with their composition, and they can have negative, neutral, or positive effects on vascular plants4,9,10,11,12,13,14; see meta-analysis and review by Havrilla et al.15. This variation in effects depends on the composition of biocrusts, vascular plant functional groups, and the provenance of the vascular plants. However, in field conditions, many studies have found that the germination and survival of native plant species is higher, or not changed, in patches with biocrusts than in area without crusts16. Their facilitative and ecosystem engineering roles have been of substantial interest because of the positive effects of biocrusts on soil water and nitrogen7,17,18,19.
Direct facilitation infers some degree of interdependence among species20,21, but simple pairwise direct facilitation can lead to facilitative “interaction chains” that can have complex, but not necessarily obligate, effects through communities. Interaction chains derived from linked direct pairwise interactions between species occur when “…one species directly alters the abundance of a second species, the change in the second species affects a third species, and so on”22. These chains can also be characterized as “facilitation cascades” when a series of successive facilitation interactions occur among nested groups of species23,24,25,26. To our knowledge, all but one of currently reported facilitation cascades have trophic dimensions and have focused on intertidal marine systems (but see Losapio et al.27). As an exception, but still trophic in nature, Angelini and Silliman28 found that Quercus virginiana facilitated epiphytes which in turn dramatically increased invertebrate feeding guilds. Facilitation cascades or positive interaction chains between plants, are likely to occur in semi-arid systems where biocrusts act as “a basal habitat former”25, analogous to Altieri et al.23 positive effects of cordgrass on mussels, for vascular plant species such as shrubs, which then may have positive effects on other species29. Biocrusts can certainly act as an ‘organizing principle’ at multiple scales1, and plant species in arid ecosystems can have strong facilitative effects on each other21. If these interactions are linked, they can likely also support facilitation cascades among autotrophs. Facilitation cascades, to date, have only focused on links that include consumers or the formation of habitats for animals, but not cascades formed only among autotrophs. Understanding the potential of biocrust-based autotrophic cascades is important30 because facilitative cascades can amplify biodiversity25,26, and because biocrusts have been extensively destroyed by livestock and recreation8,31. Further, the consequences of this damage can be exacerbated by warming temperatures32.
We explored possible facilitation cascades in semi-arid shrub steppe in western Montana dominated by the shrub Artemisia tridentata Nutt. ssp. wyomingensis Beetle & Young (Wyoming big sagebrush) and bunchgrasses, and where biocrusts commonly coexist. As noted, biocrusts can have positive and negative effects, and A. tridentata and relevant bunchgrasses have been shown to have facilitative effects on other species33,34,35,36,37,38. Intense competition is also common among these species35,36,39,40, and thus interaction cascades may not be entirely facilitative, but instead may be “realized”, which include nested negative and positive interactions46.
We integrated two-year field experiments designed to test interactions among biocrusts and two co-dominant vascular species with small-scale and large-scale measurements of spatial patterns of plants, soil water, and soil nitrogen to infer interactions among other species and at a larger spatial scale. We also conducted greenhouse experiments to measure the nitrogen-fixing capacity of the biocrusts. We organized these experiments around the following three predictions. (1) Biocrusts facilitate vascular plants. (2) Artemisia facilitates other plants and biocrusts growing under their canopies. (3) Interaction cascades initiated by biocrusts correspond with increased soil nitrogen and moisture. In total, this work identifies direct and indirect effects of biocrusts in the context of change and other key species within the region.
Methods
Site
We integrated field experiments and spatial patterns in semi-arid sagebrush steppe at Calf Creek Wildlife Management Area (CCWMA), in western Montana on the east side of the Bitterroot Valley, Montana (46.2805–114.0047; 1380 m elevation). Descriptions of the integrated experiments and spatial pattern measurements are listed in Supplementary Table 1. CCWMA is maintained as winter range for elk (Cervus elaphus), and mule deer (Odocoileus hemionus). White-tailed deer (Odocoileus virginianus) are also common at the site. Cattle are fenced out of CCWMA, but we have observed occasional bovine trespassers. Mean annual precipitation at the site is approximately 30 cm. Artemisia tridentata is dominant and occurs in a patchy pattern with areas composed of bunchgrasses and forbs. We focused on the spatial patterns of the native bunchgrasses which were the most common at the site: Festuca idahoensis Elmer (Idaho fescue), Koeleria macrantha (Ledeb.) Schult. (prairie junegrass), Pseudoroegneria spicata (Pursh) Á. Löve (bluebunch wheatgrass), Hesperostipa comata (Trin. & Rupr.) (needle-and-thread grass), Leymus cinereus (Scribn. & Merr.) (Great Basin wild rye), and Poa secunda (J. Pres) (Sandberg’s bluegrass). We then focused on F. idahoensis (hereafter Festuca) in experiments testing the effects of Artemisia and biocrusts. The large-scale patches of both Artemisia and herbaceous communities were typically 10 to > 100 m in cross section when visually surveyed during experimental setup. We also observed relatively low abundances of the exotic invaders Centaurea stoebe and Bromus tectorum.
Spatial patterns
Based on other studies finding that biocrust cover increased with shrub cover at large scales, but decreased with shrub cover at small scales43, we measured spatial patterns at the following two scales: at the large scale of patches containing many Artemisia shrubs vs. similar sized patches without any shrubs, and at the small scale of individual Artemisia understories vs. small open areas within Artemisia patches. Because our observations suggested that Artemisia might create interstitial spaces with low grass density, we sampled at the large scale to determine whether bunchgrass species or biocrusts were associated or disassociated with Artemisia patches. Because shrub canopies might shade biocrusts, we sampled at the small scale to determine whether bunchgrass species or biocrusts within patches were associated or disassociated with Artemisia individuals. At the large scale, 30 1 × 1 m quadrats were randomly located within haphazardly chosen Artemisia and grassland patches. In each quadrat, the number of each of the bunchgrass species listed above were counted and the percent cover of Artemisia and biocrust was estimated visually to the nearest 10%. We used the “basic distance” approach41 with 20 m transects run through Artemisia patches to randomly choose shrubs for spatial associations and sampled vegetation at random points chosen within each of the 5 m section blocks on each transect. We located a 30 × 30 cm quadrat under the north quadrant of each of 50 Artemisia shrubs, and a 30 × 30 quadrat in the nearest open space to the north of each of these sampled Artemisia individuals. In each quadrat, we visually estimated the percent cover of biocrust to the nearest 5% and counted all individuals of the grass species listed above. The species were separated into those with or without flower stalks. Finally, to estimate the effect of crust on juvenile Artemisia, we sampled five 20 × 100 m areas for juveniles (< 20 cm tall) and recorded whether they were in the open, under adult shrubs, or within a biocrust patch.
Field experiments
To experimentally test the effects of biocrusts on Artemisia, we established 20 30 × 30 cm plots in areas of undisturbed biocrust cover in June 1996. In half of these plots, the biocrusts were removed and in the other half biocrusts were left intact. A one-year-old Artemisia plant was transplanted into the center of each plot. These juvenile Artemisia had been grown from locally collected seed in a blend of silica sand and soils collected from the field study site in a greenhouse before transplanting. Each plot was caged with 12-gauge wire mesh to prevent trampling and herbivory by native ungulates. These transplants were left in place for two years, but after a year, our observations suggested that growth was very slow, perhaps too slow to provide detectable comparisons of biomass. Therefore, we measured photosynthetic rates of Artemisia towards the end of the growing season (mid-September) in 1996 and again in 1997 using a LI-COR 6200 portable infrared gas analysis system. We placed entire Artemisia seedling shoots into a custom-made 1.2 L cuvette and exposed them to full sunlight without cloud interference. After two years, the aboveground biomass of Artemisia transplants was harvested, dried at 60 °C, and weighed.
To experimentally test the effects of biocrusts on Festuca, we established 20 30 × 30 cm plots in areas of undisturbed biocrust cover in June 1996. In half of these plots, the biocrusts were removed and in the other half they were left intact. A one-year-old Festuca plant was transplanted into the center of each plot. These had been grown from locally collected seed in a greenhouse in a blend of silica sand and soils collected from the field study site before transplanting. Each plot was caged with a 12-gauge wire mesh to prevent trampling and herbivory by native ungulates. These transplants were in situ for two years.
To test the effect of Artemisia on Festuca, we randomly selected 60 mature Artemisia shrubs and 30 were removed and 30 left undisturbed. We also selected 60 open areas with biological soil crust, at least two meters away from the nearest Artemisia for shade treatments. Half of these 60 open areas were covered with a shade screen treatment using shade cloth that blocked approximately 30% of sunlight and half were left without shade screens. In each of these 120 plots, a single one-year old Festuca plant was transplanted. In mid-September 1997, we measured Festuca survivorship, the proportion of grazed plants, and harvested and weighed aboveground biomass. Any Festuca that showed clear signs of leaf herbivory was counted as grazed.
We measured soil moisture gravimetrically across all treatments in both the Artemisia-effect and soil crust-effect experiments. Soil samples were collected in all plots in mid-September 1997 when there had been no precipitation for over a week, from a depth of 5–10 cm. Each sample was weighed, dried at 100 °C for 2 days and weighed again for gravimetric soil moisture. In mid-October 2022, again roughly a week after light precipitation, we sampled soil from 5 to 10 cm depth beneath Artemisia, beneath biocrusts, and in the open. Samples were weighed, dried at 100 °C for 2 days and weighed again for gravimetric soil moisture.
Greenhouse experiments
We conducted a greenhouse experiment to test the effects of biocrusts on the germination and establishment of Festuca. The greenhouse was on the University of Montana campus (46.8609, − 113.98401). In June 1996, soil was collected from the field site and was added to 50 × 25 × 10 cm rectangular trays. Biocrusts removed for the field experiment were established on half of these trays, and the other half were left without biocrusts. Fifty Festuca seeds were sown in each tray, spray-misted twice per day until seeds germinated, and were subsequently watered two times per week for the duration of the experiment. Germination was monitored, and after 120 days, all surviving Festuca plants were counted again as “established”, then harvested, dried at 60 °C, and weighed.
Laboratory experiments
We conducted laboratory experiments to determine the cyanobacterial composition of biocrusts and to estimate the nitrogen-fixing capacity of biocrusts over a range of temperatures and water saturation. Biocrusts were collected from the field site. Belnap8 stated that “cyanobacteria form the matrix of biological soil crusts”, thus we cultured and isolated cyanobacterial species on 1% agar plus medium D and the dominant genera were identified using phase contrast microscopy.
For nitrogen-fixation, biocrusts were maintained in enclosed growth chambers under controlled temperature and light similar to those typical in the field. The source of photosynthetically active radiation (PAR) in the 400–700 nm range was from very high output cool-white florescent lamps. Sylvania black light fluorescent lamps (peak output 365 nm, range 280–360 nm) provided a source of UVA radiation, and Westinghouse FS—40 fluorescent lamps (peak output 310 nm, range 280–360 nm) were the source of UVB radiation. Biocrust samples used for the acetylene reduction assays were 2 × 2 cm. The cuvettes used for crusts were 12 ml, injected with 1 ml acetylene (C2H2), and incubated for 1 h. Following incubation, gas samples were withdrawn and C2H was quantified using a Gow-Mac 69–750 gas chromatograph. Biocrust nitrogenase activity was measured over a range of temperatures from 0 to 45 °C, and a range of biocrust water saturation from about 10% to full saturation.
Statistical analyses
All statistics were performed in SigmaPlot 14.5 (Systat Software, Inc. 2020) and cross-validated in R 4.2.142. Linear regressions were used to test the relationships between vascular plant cover in large-scale (> 10 m diameter) patches for both herbaceous plants and Artemisia. For the relationship between Artemisia juveniles and biocrust, we used a Chi-square test, and for comparisons of herbivory on Festuca under Artemisia and in the open we used a Fischer’s exact test. For all other pairwise comparisons, we used t-tests when data were normally distributed and Mann–Whitney Rank Sum tests when assumptions of normality were not met—these are noted in the results. For the four concomitant treatments in the field experiment testing the effects of Artemisia on Festuca, the data failed the Shapiro–Wilk normality test, so we used a Kruskal–Wallis one way ANOVA on ranks followed by Dunn’s Method pairwise multiple comparisons. Our goal for N-fixation was simply to determine the capacity of our biocrusts to provide nitrogen to the system, therefore we grouped rates by two ranges of temperatures and two ranges of water saturation and presented these rates in box plots. For comparisons of soil water among Artemisia, biocrusts and soil without biocrusts in 2022, data were not normally distributed so we used a Kruskal–Wallis one way ANOVA on ranks followed by Tukey tests for pairwise comparisons. For comparisons of ammonium and nitrate in soils we used one way ANOVA followed by Holm-Sidak pairwise multiple comparisons.
Results
Spatial patterns
Biocrust cover was 54 ± 4% in large patches of Artemisia vs. 34 ± 4% in large open patches without Artemisia (Mann–Whitney U = 68.5; P > 0.001; Fig. 1). In each patch type, increasing vascular plant cover corresponded with decreasing biocrust cover (Fig. 1). At this large scale,the six selected bunchgrass species were twice as common in open patches without Artemisia as in Artemisia patches, 19.1 ± 1.4 vs. 10.4 ± 1.9 individuals per m2 (t = − 5.199, P < 0.001). Festuca, the target experimental species, occurred at similar densities in both patch types; 3.0 ± 0.9 in herbaceous patches vs. 1.6 ± 0.4 in Artemisia patches (Mann–Whitney U = 192.0; P = 0.836).
In contrast to the large-scale patterns, at the scale of individual Artemisia shrubs, biocrusts were less abundant under Artemisia than in the open matrix surrounding Artemisia. Within Artemisia patches, and under individual Artemisia shrubs, biocrust cover was 39 ± 4% vs. 84 ± 3% in the inter-shrub open matrix (Mann–Whitney U = 204.0, P < 0.001). The richness of the six selected bunchgrass species under Artemisia was 0.9 ± 0.2 vs. 1.9 ± 0.1 per 0.25 m2 in the open matrix (Mann–Whitney U = 602.50, P < 0.001). The total density of these species was 1.9 ± 0.3 per m2 under Artemisia vs. 4.7 ± 0.4 in the open matrix (Mann–Whitney U = 531.0, P < 0.001). There were more reproductive Festuca in the open matrix than under Artemisia (0.5 ± 0.1 vs. 0.3 ± 0.1 per m2; Mann–Whitney U = 1019.000; P = 0.050), but non-reproductive Festuca were four times more abundant under Artemisia (0.8 ± 0.2 vs. 0.2 ± 0.1 per m2; Mann–Whitney U = 947.0; P = 0.004).
We recorded 23 juvenile Artemisia in Artemisia patches, none of which were under mature Artemisia or in open soils without biocrust, and all of which were in biocrusts. Based on this observed number and the expected numbers derived from the 84% biocrust cover in the interstitial spaces among Artemisia shrubs, biocrusts correlated positively with the recruitment of Artemisia (Chi-square test, χ2 = 4.41, P = 0.036).
Field experiments
The aboveground biomass of juvenile Artemisia planted in biocrusts did not differ from that of Artemisia planted where biocrusts had been removed (Fig. 2a; t = 0.386; df = 2,14; P = 0.706). However, these same Artemisia planted in biocrusts photosynthesized at higher rates in 1997 than those planted where biocrusts had been removed (Fig. 2c; t = 6.301; df = 2,11; P < 0.001), but this was not the case in 1996 (Fig. 2b).
Festuca planted in biological soil crust was almost twice as large after two growing seasons as those growing in no biological soil crust treatments (Mann–Whitney U = 55.0; df = 2, 39; P < 0.001; Fig. 3).
Artemisia facilitated Festuca after two growing seasons, as Festuca biomass under Artemisia canopies was roughly twice as much as those in the open (Kruskal–Wallis ANOVA on Ranks, Dunn’s pairwise, Q = 2.815, P = 0.029; Fig. 4). Festuca biomass under Artemisia canopies was also different than that of Festuca planted under shade placed in the open (Q = 3.494, P = 0.003). However, the biomass of Festuca planted under Artemisia canopies was not different than those planted where Artemisia had been removed (Q = 1.960, P = 0.300).
Greenhouse experiment
Biocrusts strongly facilitated Festuca in the greenhouse experiment. When grown in crusts, Festuca germination was roughly five times higher than without crusts (Fig. 5; Mann–Whitney U = 55.000, P < 0.001), and establishment was almost four times greater in the biocrust treatment (t = 4.538; df = 2,38; P < 0.001).
Potential mechanisms
Nitrogen
Laboratory cultures of biological soil crust cyanobacterial species found four predominant genera of cyanobacteria in the biocrusts. Two of these genera, Scytonema and Nostoc, are strong nitrogen fixers, and Microcoleus and Phormidium are weak nitrogen fixers. Laboratory assays of biocrust nitrogen fixation suggest that under a broad range of natural field temperature and moisture conditions, sagebrush steppe biocrusts fix a potentially significant amount of nitrogen (Fig. S1). In 2022, concentrations of ammonium did not differ among soils under biocrusts, under Artemisia or in the open (Fig. 6; one way ANOVA, F = 1.365; df = 1,29; P = 0.271). Nitrate concentrations were higher under biocrusts (t = 2.917; P = 0.020) and Artemisia (t = 2.376; P = 0.048) than in the open (F = 4.754; df = 1,29; P = 0.016). There was no difference in nitrate in soils beneath biocrusts and Artemisia (t = 0.435; P = 0.667).
Water
In 1997, when soils were much wetter overall, biocrusts had no effect on soil moisture content in mid-September 1997, with the mean percent water content of soil under biocrusts at 11.8 ± 1.1% and soil without biocrusts at 11.7 ± 1.5% (t = 0.0408; df = 26.1, P = 0.968). In 2022, when soils were much drier overall, soil moisture was 4.0 ± 0.7% under biocrusts vs. 2.3 ± 1.3% in the open without biocrusts (Kruskal–Wallis one way ANOVA on Ranks, H = 11.821, df = 2, P = 0.003; Tukey test = 0.003). In 1997, soil moisture under Artemisia did not differ from that in the open (11.5 ± 0.5 vs. 11.0 ± 0.4; t = 0.713, df = 3,61, P = 0.479). In 2022, soil moisture was 2.4 ± 0.2% under Artemisia, which did not differ from that in the open without biocrusts (Tukey test, P = 0.712), but was lower than that under biocrusts (Tukey test, P = 0.033).
Herbivory
Of the surviving Festuca planted under Artemisia, 32% (9 of 28) were damaged by herbivores, vs. 83% (19 of 23) in the open matrix (Fischer’s exact test = 0.002; P < 0.001).
Discussion
The most salient aspect of our results is that biocrusts facilitated a key vascular plant in this system, the shrub Artemisia tridentata. Through this facilitation, biocrusts appeared to amplify a cascade of extended effects ranging from facilitative effects on Festuca idahoensis to apparent competitive effects on other bunchgrasses, juvenile Artemisia, and biocrusts themselves; the latter three all based on spatial patterns (Fig. 7). Importantly, the effect of Artemisia on biocrusts may depend on the scale at which patterns are measured. At larger scales, the negative relationship between Artemisia shrubs and grasses in general appeared to create interstitial spaces around shrubs that actually increased the abundance of biocrusts in large shrubby patches relative to large herbaceous patches, despite the negative effects of individual shrubs within large patches. This may be because bunchgrass density was roughly four times less in these interstitial spaces than in the large open patches without Artemisia. Soliveres and Eldridge43 described similar scale-based differences in positive and negative spatial associations among shrubs and biocrusts in Australia, with biocrust cover and diversity increasing with shrub cover at large scales, and negative effects at small scales.
In addition to indirect facilitation through Artemisia, biocrusts also strongly directly facilitated Festuca, evidenced by higher germination and establishment in the greenhouse, and higher growth in the field experiment. Secondary components of cascades included facilitative effects of Artemisia on Festuca—increased growth of Festuca under Artemisia in field experiments and spatial associations between non-flowering Festuca and Artemisia shrubs in the field. Other cascading outcomes, based on spatial associations, included the apparent suppression of other bunchgrasses and juvenile Artemisia by adult Artemisia, with all suggesting that biocrusts may drive important cascades in intermountain shrub steppe.
Components of these chains of interactions are consistent with facilitation cascades described in shallow intertidal zones and sea beds23,44. The latter described a scenario in which cordgrass establishes and then facilitates a number of other species including mussels, snails, and seaweeds. Much like Artemisia in our case, facilitated mussels increase the densities of other species such as amphipods and barnacles (also see Altieri et al.24). They suggested that cordgrass acted as a “primary facilitator”, perhaps like biocrusts, and mussels acted as a “secondary facilitator”, perhaps like Artemisia. An important difference in our system, however, was that biocrust-facilitated Artemisia appeared to have many negative, or competitive, effects on most bunchgrass species. Thus, our results might be more like results for “multiple, independent cascades” in mangrove-dominated systems that appeared to be initiated by “a single basal facilitator”45. The spatial patterns of the large suite of species we measured also suggest a cascade more in line with that of Gribben et al.46. They presented evidence for “a realized facilitation cascade” as a “function of nested negative and positive interactions” that vary as the density of key species varies, rather than a “collection of hierarchical positive interactions”. Although we do not explore this, these complex negative and positive interactions have the potential to maintain community diversity through intransitive interactions47.
Other facilitation cascades in the literature generally have more obligate relationships than the biocrust → Artemisia → Festuca cascade explored here. In part, this may be because none of our cascade components are consumers that can obviously have strong, obligate relationships with prey abundances. Each of our autotrophic components can certainly exist without the others, thus, the cascades suggested by our results are relatively more facultative and contextual than others reported in the literature.
The variable negative and positive interactions in a realized facilitation cascade46 might be related to facilitation and competition being highly context dependent, and such context dependence appears to be very much the case for the effects of biocrusts in general15. In a large-scale multi-factorial field experiment in intermountain prairie, vegetation very similar to ours, biocrusts inhibited the overall emergence of four different exotic seedlings in a wet year, but not a dry year, and had no effect on the emergence of four different native species9. The effects of biocrusts in rosemary scrub in Florida vary among species, distance from shrubs, and time since fire48. Experimental removal of biocrusts from around established Bouteloua gracilis bunchgrasses reduced their performance49. These kinds of variable effects are highlighted in a meta-analytical review which found that biocrusts dominated by lichens, such as ours, inhibited overall plant performance—on average15. In this review, biocrusts had more positive effects on plant species without mutualistic nitrogen-fixing bacteria, such as our Festuca and Artemisia than species with these symbionts. More importantly, they found that lichen-dominated biocrusts across studies suppressed germination, the opposite of what we found in the greenhouse experiment for germination and establishment, but improved growth, as we found in the field for Festuca, and corresponded with the higher photosynthetic rates of Artemisia in biocrusts in the field.
The effects of Artemisia on native bunchgrasses are less studied than those of biocrusts, but they are also variable. Early studies indicated that Artemisia effects are generally, but conditionally, competitive39,50,51, and in a detailed experimental study the roots of Artemisia outcompeted those of P. spicata for soil nitrogen40. However, others have found that Poa secunda, a species apparently excluded from Artemisia understories in our study, was more common under Artemisia than in the open, but only at the driest sites52. In this study, Elymus elymoides abundance was higher under Artemisia canopies than in open interspaces at both drier and wetter sites. In experimental manipulations, Poulos et al.53 found that Artemisia facilitated the native forb, Penstemon palmeri, but this generated complex interactions with other herbaceous species in the understory, another aspect of interaction cascades that we did not pursue. In eastern Oregon, a wide range of different functional groups of vascular plants are positively associated with Artemisia37 much as found for many other shrub species54,55. Furthermore, there is evidence that Artemisia can have positive allelopathic (i.e., biochemically mediated) effects on conspecifics56 and negative allelopathic effects on understory grasses57. As a group, our six selected native bunchgrass species were negatively associated with Artemisia. All of this variability in shrub effects considered the facilitative effect of biocrusts on Artemisia clearly has the potential to affect a diverse variety of interaction cascades in addition to ours. In fact, although we focused solely upon autotrophs in our study, because less is known about cascades among autotrophs, Artemisia is a keystone species for consumers. Artemisia provides habitat for obligate consumers including the greater sage-grouse (Centrocerus urophasianus), sagebrush sparrow (Artemisiospiza nevadensis), sage thrasher (Oreoscoptes montanus), and pronghorn antelope (Antilocapra americana)58. As such, the maintenance of larger scale community structure and diversity in sagebrush steppe may be affected by even more extended and trophic biocrust-Artemisia facilitation cascades.
Our results indicating a positive effect of biocrusts on Artemisia are, to our knowledge, somewhat atypical in the literature; however, a broad survey across the Great Basin found that biological crusts correlated positively with greater growth of sagebrush canopies59. Zhang and Belnap60 found that biocrusts improved the concentration of various nutrients in the leaf tissue of two Asian shrub species. And in a meta-analysis, averaged across all biocrust types, biocrusts facilitated the growth of non-nitrogen-fixing shrubs, such as Artemisia15.
Artemisia tridentata with biocrusts cover vast parts of western North America, and yet we know surprisingly little about the effects of this shrub species on biocrusts. Condon et al.61 found that Artemisia-dominated plant communities were associated with “rapidly reproducing biocrusts”. Navas Romeroa et al.62 found positive spatial associations among different shrub species and biocrusts at some sites, but neutral associations at other sites. Others have reported higher biocrust development under Artemisia ordosica canopies in China63,64,65. In New South Wales, Australia increasing shrub cover, at the large scale of “sites”, corresponded with increasing biocrust richness and cover, much like the large-scale patterns we report here43. However, much as we found at small scales, biocrusts responded negatively to shrubs as compared to open patches43.
In our study, biocrust cover decreased as vascular plant cover increased in both large-scale Artemisia and large-scale herbaceous patches, consistent with the small-scale negative relationship between Artemisia individuals and biocrust cover. She et al.66 found that biocrust cover rapidly increased with the cover of shrubs, dominated by A. ordosica when shrub productivity was low. As shrub productivity increased, the cover of biocrust did not decrease, but stabilized. In contrast, and like our results, biocrusts were abundant at low herbaceous productivity but declined rapidly with increasing herbaceous productivity. This corresponded with a strong decline in light associated with increasing herbaceous productivity, but not with increasing shrub productivity. One overlooked potential component of our cascade is that Artemisia may have substantially altered the composition of biocrusts, which might have had consequences for vascular plants, but which we did not measure. In the field, we observed that biocrusts under mature Artemisia canopies appeared to contain much more moss, Tortula roralis, than biocrusts in the open. Moss dominated crusts might have weaker positive or stronger competitive effects on vascular plants than cyanobacteria and lichen-dominated biocrusts, which were more common in the open, and with which we conducted laboratory measurements.
Biocrusts can improve the retention of soil water and increase concentrations of soil nitrogen1,6,19. Our measurements were only taken as snapshots in time but suggest that both of these processes may have been important. When soils were wet, in 1997, there were no differences in percent moisture among Artemisia, biocrusts, and open soil with no biocrusts. But, in 2022, when soils were much drier, soil under biocrusts was wetter than either in the open or under Artemisia, suggesting that biocrusts may help retain water. Also in 2022, soil nitrate concentrations were almost three times higher under biocrust as in the open, but nitrate under Artemisia was as high as under biocrusts. Interestingly, the mechanism by which Artemisia facilitated Festuca also appeared to be substrate related, perhaps via nitrogen as suggested by our results; i.e., in treatments where Artemisia was removed the biomass of Festuca was not significantly different than where it was not removed, perhaps due to its legacy effects on soils. Furthermore, when shade was provided to open sites where shrubs had not affected soils, Festuca biomass did not increase. However, shrubs can affect other species via many other effects on substrate that we did not measure67. Furthermore, because the species involved have different lifespans and recruitment requirements, these effects must have temporal components which are best considered in the context of priority effects68 but exploring priority effects are beyond the scope of this study.
For simplicity, we treated biocrusts as a single entity, but biocrusts are exceedingly complex communities themselves, being dynamic combinations of nitrogen-fixing cyanobacteria and cyanolichens, other bacteria, green algae, fungi, mosses, and liverworts5. Pietrasiak et al.5 compared functions among 10 different categories of biocrusts, each which certainly can vary in composition, and found that cyanolichen-dominated crusts fixed nitrogen at higher rates than all other crust types, photosynthesized at higher rates than most other crust types, and generated soil aggregate stability as high as any other crust type. The biocrusts in the open at our sites were dominated by lichens and combined with our identification of cyanobacteria indicate that we predominantly measured cyanolichen crusts. Importantly, different biocrusts, or different compositions of cyanolichen crusts, could potentially generate different and unique interaction chains and cascades, and this is an opportunity for deeper context specific research in arid and semi-arid systems that support biocrusts.
Data availability
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Acknowledgements
We thank Morgan McLeod at MPG Ranch for nitrogen analyses. CJL was funded by an NSERC DG. RMC was supported by National Science Foundation EPSCoR Cooperative Agreement OIA-1757351.
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W.M.R., R.M.C. and C.J.L. developed the conceptual framework and organization of the paper. R.M.C. and W.M.R. collected field spatial data and WMR designed and conducted all experiments. R.M.C. did the statistics with supervision and crosschecking by C.J.L. All authors wrote the paper.
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Ridenour, W.M., Lortie, C.J. & Callaway, R.M. A realized facilitation cascade mediated by biological soil crusts in a sagebrush steppe community. Sci Rep 13, 4803 (2023). https://doi.org/10.1038/s41598-023-31967-0
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DOI: https://doi.org/10.1038/s41598-023-31967-0
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